In silico cloning and B/T cell epitope prediction of triosephosphate isomerase from Echinococcus granulosus

Wang, Fen; Yue, Bin

doi:10.1007/s00436-016-5166-3

Cited by 8 publications

(4 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…There is no information regarding TPI as a vaccine target in toxoplasmosis. However, the present data regarding the activation of the immune system and immunomodulatory properties by this stage-specific protein and the recent suggestions for TPI as a vaccine target in helminth and Leishmania parasites might further reinforce the selection of this protein as a promising vaccine candidate against toxoplasmosis [96][97][98][99].…”

Section: Triose-phosphate Isomerase (Tpi)mentioning

confidence: 62%

Mining the Proteome of Toxoplasma Parasites Seeking Vaccine and Diagnostic Candidates

Rashidi

Sánchez-Montejo

Mansouri

et al. 2022

Animals

View full text Add to dashboard Cite

Toxoplasma gondii is a pathogenic protozoan parasite that infects the nucleated cells of warm-blooded hosts leading to an infectious zoonotic disease known as toxoplasmosis. The infection outcomes might be severe and fatal in patients with immunodeficiency, diabetes, and pregnant women and infants. The One Health approach to toxoplasmosis highlights that the health of humans is closely related to the health of animals and our common environment. The presence of drug resistance and side effects, the further improvement of sensitivity and specificity of serodiagnostic tools and the potentiality of vaccine candidates to induce the host immune response are considered as justifiable reasons for the identification of novel targets for the better management of toxoplasmosis. Thus, the identification of new critical proteins in the proteome of Toxoplasma parasites can also be helpful in designing and test more effective drugs, vaccines, and diagnostic tools. Accordingly, in this study we present important proteins found in the proteome of the life cycle-specific stages of Toxoplasma parasites that are potential diagnostic or vaccine candidates. The current study might help to understand the complexity of these parasites and provide a possible source of strategies and biomolecules that can be further evaluated in the pathobiology of Toxoplasma parasites and for diagnostics and vaccine trials against this disease.

show abstract

Section: Triose-phosphate Isomerase (Tpi)mentioning

confidence: 62%

Mining the Proteome of Toxoplasma Parasites Seeking Vaccine and Diagnostic Candidates

Rashidi

Sánchez-Montejo

Mansouri

et al. 2022

Animals

View full text Add to dashboard Cite

show abstract

“…Specific epitopes determine the specificity of antigens ( 33 ). Therefore, epitope prediction is particularly important in the study of protein structure and function, and for the diagnosis of diseases and the design of vaccines ( 34 ). The present study found that there were seven potential B-cell epitopes in Eg-TSP11, among which the random coil regions were 227–240 (amino acid sequence: WQYGPPRFTNGAHN) and 210–219 (amino acid sequence: LTGWPTDQYQ).…”

Section: Discussionmentioning

confidence: 99%

Molecular Characterization of a Tetraspanin TSP11 Gene in Echinococcus granulosus and Evaluation Its Immunoprotection in Model Dogs

et al. 2021

View full text Add to dashboard Cite

Cystic echinococcosis (CE) is a cosmopolitan zoonosis caused by the larval stage of Echinococcus granulosus, which affects humans and a wide range of mammalian intermediate hosts. Parasite tetraspanin proteins are crucial for host-parasite interactions, and therefore they may be useful for vaccine development or disease diagnosis. In the present study, the major antigen coding sequence of tetraspanin 11 (Eg-TSP11) from E. granulosus was determined. The results of immunolocalization showed that Eg-TSP11 was mainly located in the tegument of adult worms and protoscoleces. Western blotting analysis showed that the serum from dogs injected with recombinant Eg-TSP11 (rEg-TSP11) could recognize Eg-TSP11 among natural protoscolex proteins. Moreover, the serum from dogs with E. granulosus infection also recognized rEg-TSP11. Serum indirect enzyme-linked immunosorbent assays demonstrated that IgG levels gradually increased after the first immunization with rEg-TSP11 compared with those in the control group. Furthermore, the serum levels of interleukin 4, interleukin 5, and interferon gamma were significantly altered in the rEg-TSP11 group. Importantly, we found that vaccination with rEg-TSP11 significantly decreased worm burden and inhibited segment development in a dog model of E. granulosus infection. Based on these findings, we speculated that rEg-TSP11 might be a potential candidate vaccine antigen against E. granulosus infection in dogs.

show abstract

“…Furthermore, several of the hereidentified proteins have been proposed as vaccine candidates against E. granulosus. 4,45,46 Among them, Eg.P29-also known as endophilin B1 (#17 in our set) -revealed significant protective activity against secondary infection in mice, 47 as well as against primary infection in sheep, 48 inducing in both cases roughly 95% protection levels. According to our classification, this protein is "unique" (US: 0.24) and highly antigenic (ANTIGENpro score: 0.94), showing an AAR value (42.2) similar to the one obtained for the whole PSEx proteome.…”

Section: Discussionmentioning

confidence: 99%

“…Among the 58 proteins we reported for PSEx, 36 of them were previously identified in at least one of the abovementioned studies, while 22 proteins were described for the first time in the present work (Table ). Furthermore, several of the here‐identified proteins have been proposed as vaccine candidates against E. granulosus . Among them, Eg.P29—also known as endophilin B1 (#17 in our set)—revealed significant protective activity against secondary infection in mice, as well as against primary infection in sheep, inducing in both cases roughly 95% protection levels.…”

Section: Discussionmentioning

confidence: 99%

Combining proteomics and bioinformatics to explore novel tegumental antigens as vaccine candidates against Echinococcus granulosus infection

Miles

Portela

Cyrklaff

et al. 2019

J of Cellular Biochemistry

View full text Add to dashboard Cite

Echinococcus granulosus is the parasite responsible for cystic echinococcosis (CE), an important worldwide‐distributed zoonosis. New effective vaccines against CE could potentially have great economic and health benefits. Here, we describe an innovative vaccine design scheme starting from an antigenic fraction enriched in tegumental antigens from the protoscolex stage (termed PSEx) already known to induce protection against CE. We first used mass spectrometry to characterize the protein composition of PSEx followed by Gene Ontology analysis to study the potential Biological Processes, Molecular Functions, and Cellular Localizations of the identified proteins. Following, antigenicity predictions and determination of conservancy degree against other organisms were determined. Thus, nine novel proteins were identified as potential vaccine candidates. Furthermore, linear B cell epitopes free of posttranslational modifications were predicted in the whole PSEx proteome through colocalization of in silico predicted epitopes within peptide fragments identified by matrix‐assisted laser desorption/ionization‐TOF/TOF. Resulting peptides were termed “clean linear B cell epitopes,” and through BLASTp scanning against all nonhelminth proteins, those with 100% identity against any other protein were discarded. Then, the secondary structure was predicted for peptides and their corresponding proteins. Peptides with highly similar secondary structure respect to their parental protein were selected, and those potentially toxic and/or allergenic were discarded. Finally, the selected clean linear B cell epitopes were mapped within their corresponding 3D‐modeled protein to analyze their possible antibody accessibilities, resulting in 14 putative peptide vaccine candidates. We propose nine novel proteins and 14 peptides to be further tested as vaccine candidates against CE.

show abstract

In silico cloning and B/T cell epitope prediction of triosephosphate isomerase from Echinococcus granulosus

Cited by 8 publications

References 17 publications

Mining the Proteome of Toxoplasma Parasites Seeking Vaccine and Diagnostic Candidates

Mining the Proteome of Toxoplasma Parasites Seeking Vaccine and Diagnostic Candidates

Molecular Characterization of a Tetraspanin TSP11 Gene in Echinococcus granulosus and Evaluation Its Immunoprotection in Model Dogs

Combining proteomics and bioinformatics to explore novel tegumental antigens as vaccine candidates against Echinococcus granulosus infection

Contact Info

Product

Resources

About