In silico and in vivo assessment of developmental toxicity, oxidative stress response &amp; Na+/K+-ATPase activity in zebrafish embryos exposed to cypermethrin

Gupta, Priya; Mahapatra, Archisman; Suman, Anjali; Singh, Rahul Kumar

doi:10.1016/j.ecoenv.2023.114547

Cited by 12 publications

(4 citation statements)

References 56 publications

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“…In addition, changes in acetylcholine esterase, nitric oxide content, and Na + /K + -ATPase activity were observed. These changes disrupted cardiac development and ion regulation ( Gupta et al, 2023 ). Acute deltamethrin exposure elevated malondialdehyde levels and lowered catalase enzyme.…”

Section: Pesticides Induced Oxidative Stress In Fishesmentioning

confidence: 99%

Effects of microplastics, pesticides and nano-materials on fish health, oxidative stress and antioxidant defense mechanism

Subaramaniyam,

Allimuthu,

Vappu

et al. 2023

Front. Physiol.

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Microplastics and pesticides are emerging contaminants in the marine biota, which cause many harmful effects on aquatic organisms, especially on fish. Fish is a staple and affordable food source, rich in animal protein, along with various vitamins, essential amino acids, and minerals. Exposure of fish to microplastics, pesticides, and various nanoparticles generates ROS and induces oxidative stress, inflammation, immunotoxicity, genotoxicity, and DNA damage and alters gut microbiota, thus reducing the growth and quality of fish. Changes in fish behavioral patterns, swimming, and feeding habits were also observed under exposures to the above contaminants. These contaminants also affect the Nrf-2, JNK, ERK, NF-κB, and MAPK signaling pathways. And Nrf2-KEAP1 signalling modulates redox status marinating enzymes in fish. Effects of pesticides, microplastics, and nanoparticles found to modulate many antioxidant enzymes, including superoxide dismutase, catalase, and glutathione system. So, to protect fish health from stress, the contribution of nano-technology or nano-formulations was researched. A decrease in fish nutritional quality and population significantly impacts on the human diet, influencing traditions and economics worldwide. On the other hand, traces of microplastics and pesticides in the habitat water can enter humans by consuming contaminated fish which may result in serious health hazards. This review summarizes the oxidative stress caused due to microplastics, pesticides and nano-particle contamination or exposure in fish habitat water and their impact on human health. As a rescue mechanism, the use of nano-technology in the management of fish health and disease was discussed.

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Section: Pesticides Induced Oxidative Stress In Fishesmentioning

confidence: 99%

Effects of microplastics, pesticides and nano-materials on fish health, oxidative stress and antioxidant defense mechanism

Subaramaniyam,

Allimuthu,

Vappu

et al. 2023

Front. Physiol.

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“…The health of fish can be seriously affected after exposure to cypermethrin (CM), a well-known type II pyrethroid, since it drastically disrupts several physiological and biochemical processes by modulating different signalling cascades in fish. [3][4][5] Therefore, it is imperative to take prompt action to counter or avoid the harmful effects of these pesticides related to trophic level-oriented bioaccumulation and biomagnification resulting in serious health complications.…”

Section: Introductionmentioning

confidence: 99%

Nano-pelargonidin modulates p53/PARP/HSP 90/XRCC1 signaling axis to combat cypermethrin-induced genotoxicity and metabolic dysfunction in fish: a molecular docking predicted in vivo approach

Sow,

Dey,

Dey

et al. 2024

Environ. Sci.: Nano

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“…47,48 Recent findings shows that zebrafish, Danio reiro, embryos exposed to cypermethrin display reduced NKA enzyme activity. 92 Similarly, NKA activity decrease in Indian stinging catfish, Heteropneustes fossilis, exposed to elevated lead (Pb) levels suggests that NKA enzyme activity or NKA subunit expression may be sensitive biomarkers for a wide range of environmental toxins. 93 The response in NKA activity and subunit expression may either be a direct effect of changes in concentrations of water constituents themselves, or an indirect and/or additive effect of elevated levels of circulating stress hormones, particularly cortisol.…”

Section: Variations In the Rearing Conditions Impact Nka Enzyme Activitymentioning

confidence: 99%

“…Interestingly, the decrease in gill NKA α1b‐subunit expression in smolts experiencing suboptimal water qualities are replaced by a compensatory increase of α1c isoform expression, suggesting a certain plasticity in recovering from suboptimal water quality exposure 47,48 . Recent findings shows that zebrafish, Danio reiro , embryos exposed to cypermethrin display reduced NKA enzyme activity 92 . Similarly, NKA activity decrease in Indian stinging catfish, Heteropneustes fossilis , exposed to elevated lead (Pb) levels suggests that NKA enzyme activity or NKA subunit expression may be sensitive biomarkers for a wide range of environmental toxins 93 .…”

Section: Evaluation Of Sw Tolerance and Smolt Quality In Modern Aquac...mentioning

confidence: 99%

New wine in old bottles: Modification of the Na⁺/K⁺‐ATPase enzyme activity assay and its application in salmonid aquaculture

Takvam,

Sundell,

Sundh

et al. 2023

Reviews in Aquaculture

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The Na+, K+ ATPase (NKA) enzyme is important to generate the transmembrane ion gradient in the gills, intestine, and kidneys, hence, is vital for secondary transport of fluids and different solutes in teleosts. Gill NKA enzyme activity is often used as a proxy for parr–smolt transformation (PST) during which anadromous salmonids prepare for seawater (SW). Increased intensification and production of larger smolts in modern salmonid aquaculture has resulted in reports of gill NKA activity being less reliable as a proxy for smolt quality. Consequently, changes in mRNA nka‐α1b/α1a ratios in gills are increasingly used as indicators of PST. However, nka isoform mRNA abundance may not reflect translation into the functional protein, nor the activity of the mature enzyme. This may limit the predictive power of molecular markers under certain environmental conditions, rearing regimes and biological scenarios. During PST, the osmoregulatory transformations necessary for SW tolerance and survival does not only occur in the gills. Equally important are the changes in ion transporting activities, including NKA activity, in the intestine and kidneys. However, to our knowledge, there are no previous studies addressing the timing and concurrent changes in NKA activity in the three osmoregulatory tissue during PST. Here we present modifications and optimization of the NKA enzyme activity protocols for gill, intestinal and kidney tissue and outline how to best utilize NKA activity measurements as part of a more holistic approach to evaluate overall smolt quality in modern aquaculture.

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In silico and in vivo assessment of developmental toxicity, oxidative stress response & Na+/K+-ATPase activity in zebrafish embryos exposed to cypermethrin

Cited by 12 publications

References 56 publications

Effects of microplastics, pesticides and nano-materials on fish health, oxidative stress and antioxidant defense mechanism

Effects of microplastics, pesticides and nano-materials on fish health, oxidative stress and antioxidant defense mechanism

Nano-pelargonidin modulates p53/PARP/HSP 90/XRCC1 signaling axis to combat cypermethrin-induced genotoxicity and metabolic dysfunction in fish: a molecular docking predicted in vivo approach

New wine in old bottles: Modification of the Na⁺/K⁺‐ATPase enzyme activity assay and its application in salmonid aquaculture

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In silico and in vivo assessment of developmental toxicity, oxidative stress response & Na+/K+-ATPase activity in zebrafish embryos exposed to cypermethrin

Cited by 12 publications

References 56 publications

Effects of microplastics, pesticides and nano-materials on fish health, oxidative stress and antioxidant defense mechanism

Effects of microplastics, pesticides and nano-materials on fish health, oxidative stress and antioxidant defense mechanism

Nano-pelargonidin modulates p53/PARP/HSP 90/XRCC1 signaling axis to combat cypermethrin-induced genotoxicity and metabolic dysfunction in fish: a molecular docking predicted in vivo approach

New wine in old bottles: Modification of the Na+/K+‐ATPase enzyme activity assay and its application in salmonid aquaculture

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Product

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New wine in old bottles: Modification of the Na⁺/K⁺‐ATPase enzyme activity assay and its application in salmonid aquaculture