In silico analysis of two Haemonchus spp. serine protease peptides (S28) and their immunomodulatory activity in vitro

Camas-Pereyra, René; Bautista-García, Génesis A.; Ávila, Guillermina; Alcalá-Canto, Yazmín; Maza-Lopez, Jocelyn; Reyes-Guerrero, David Emanuel; Higuera-Piedrahita, Rosa Isabel; López-Arellano, María Eugenia

doi:10.1016/j.molbiopara.2023.111545

Cited by 4 publications

(2 citation statements)

References 29 publications

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“…A total of 3 µg of total protein were used for SDS-PAGE, and 1 µg was added to the zymogram gel for each treatment. The treatments evaluated in both gels were established as follows: PDB with fungus, PDB without fungus, SPDB with fungus, SPDB without fungus, Cz-DoxB with fungus, and Cz-DoxB without fungus [30].…”

Section: Protein Profile and Protease Activity Analysis Using Liquid ...mentioning

confidence: 99%

First Record of Flavocillium subprimulinum (Cordycipitaceae, Hypocreales) in Mexico: Morphological and Molecular Characterisation, Nematocidal Activity of Its Liquid Culture Filtrates against Haemonchus contortus and Protease Activity

Pérez-Anzúrez,

Mendoza-de Gives,

Olmedo-Juárez

et al. 2024

JoF

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This is the first record of the fungus Flavocillium subprimulinum in Mexico. The isolate was taxonomically characterised and cultured in potato dextrose broth (PDB), Czapek–Dox broth (CzDoxB), and sweet potato dextrose broth (SPDB) to obtain its filtrates (FLCF). The nematocidal activity (NA) of three FLCF concentrations was assessed against Haemonchus contortus L3. Protease activity (PA) was assessed with SDS-PAGE, followed by a zymogram. The NA of the FLCF reached 94.43% in PDB and 95.82% in CzDoxB, respectively, at 100 mg/mL. Lower mortality (64%) was found in SPDB at 100 mg/mL. SDS-PAGE showed bands (in PBS) of ~25, ~40, and ~55 kDa. The zymogram showed protein bands (PBs) with PA in the media, including PBs of ~14, ~40, and ~55 kDa. This study establishes the basis for exploring the potential use of this fungus against H. contortus, which is considered the most pathogenic parasite affecting lambs.

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Section: Protein Profile and Protease Activity Analysis Using Liquid ...mentioning

confidence: 99%

First Record of Flavocillium subprimulinum (Cordycipitaceae, Hypocreales) in Mexico: Morphological and Molecular Characterisation, Nematocidal Activity of Its Liquid Culture Filtrates against Haemonchus contortus and Protease Activity

Pérez-Anzúrez,

Mendoza-de Gives,

Olmedo-Juárez

et al. 2024

JoF

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“…[18,115]). The disruption or interruption of the functions of such proteases markedly affects a haematophagous parasite's ability to acquire critical nutrients from blood, which is why previous efforts have targeted selected proteases as vaccine molecules or candidates (e.g., [118][119][120][121][122][123][124]). For example, the metallo-protease (family M01) represented by HCON_00156280 [125] is a membrane-bound glycoprotein H11 and a known vaccine molecule.…”

Section: Key Protease Gene Families Associate With Key Biological Fun...mentioning

confidence: 99%

Genome-Wide Analysis of Haemonchus contortus Proteases and Protease Inhibitors Using Advanced Informatics Provides Insights into Parasite Biology and Host–Parasite Interactions

Zheng,

Young,

Song

et al. 2023

IJMS

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Biodiversity within the animal kingdom is associated with extensive molecular diversity. The expansion of genomic, transcriptomic and proteomic data sets for invertebrate groups and species with unique biological traits necessitates reliable in silico tools for the accurate identification and annotation of molecules and molecular groups. However, conventional tools are inadequate for lesser-known organismal groups, such as eukaryotic pathogens (parasites), so that improved approaches are urgently needed. Here, we established a combined sequence- and structure-based workflow system to harness well-curated publicly available data sets and resources to identify, classify and annotate proteases and protease inhibitors of a highly pathogenic parasitic roundworm (nematode) of global relevance, called Haemonchus contortus (barber’s pole worm). This workflow performed markedly better than conventional, sequence-based classification and annotation alone and allowed the first genome-wide characterisation of protease and protease inhibitor genes and gene products in this worm. In total, we identified 790 genes encoding 860 proteases and protease inhibitors representing 83 gene families. The proteins inferred included 280 metallo-, 145 cysteine, 142 serine, 121 aspartic and 81 “mixed” proteases as well as 91 protease inhibitors, all of which had marked physicochemical diversity and inferred involvements in >400 biological processes or pathways. A detailed investigation revealed a remarkable expansion of some protease or inhibitor gene families, which are likely linked to parasitism (e.g., host–parasite interactions, immunomodulation and blood-feeding) and exhibit stage- or sex-specific transcription profiles. This investigation provides a solid foundation for detailed explorations of the structures and functions of proteases and protease inhibitors of H. contortus and related nematodes, and it could assist in the discovery of new drug or vaccine targets against infections or diseases.

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Molecular signatures of Haemonchus contortus infection in sheep: A comparative serum proteomic study on susceptible and resistant sheep breeds

Chagas,

Ribeiro,

Osório

et al. 2024

Veterinary Parasitology

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In silico analysis of two Haemonchus spp. serine protease peptides (S28) and their immunomodulatory activity in vitro

Cited by 4 publications

References 29 publications

First Record of Flavocillium subprimulinum (Cordycipitaceae, Hypocreales) in Mexico: Morphological and Molecular Characterisation, Nematocidal Activity of Its Liquid Culture Filtrates against Haemonchus contortus and Protease Activity

First Record of Flavocillium subprimulinum (Cordycipitaceae, Hypocreales) in Mexico: Morphological and Molecular Characterisation, Nematocidal Activity of Its Liquid Culture Filtrates against Haemonchus contortus and Protease Activity

Genome-Wide Analysis of Haemonchus contortus Proteases and Protease Inhibitors Using Advanced Informatics Provides Insights into Parasite Biology and Host–Parasite Interactions

Molecular signatures of Haemonchus contortus infection in sheep: A comparative serum proteomic study on susceptible and resistant sheep breeds

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