In silico analysis of phag-like protein in Ralstonia Euthropa H16, potentially involved in polyhydroxyalkanoates synthesis

Abstract

Polyhydroxyalkanoates (PHA) are synthesised by bacteria as carbon storage material. The protein PhaG directs carbon from non-related carbon sources such as glycerol, metabolised through fatty acid de novo synthesis (FAS) pathway, with PHA synthesis. The gene that codifies for this protein has not yet been found in the genome of Ralstonia eutropha H16, a model organism. By bioinformatic comparison to already known PhaG proteins, a PhaG-like protein was found codified by gene H16_A0147 and presence of the gene was preliminary confirmed by PCR. This is the first study that shows the presence and characteristics of a PhaG-like protein in R. eutropha H16 and represents the first step for the identification of a connection between FAS and PHA pathways in this model bacterium. Further gene deletion and enzymatic activity studies are necessary to confirm this potential relationship, which could improve industrial PHA production and utilisation of agro-industrial residues such as glycerol.

Demystifying polyphosphate-accumulating organisms relevant to wastewater treatment: A review of their phylogeny, metabolism, and detection

“…The PHA gene cluster typically includes phaC , phaA , phaB , phaG , and phaJ [ 61 ]. The genes phaA , phaB , phaG , and phaJ encode for a group of enzymes that are essential for producing PHA monomers from different carbon sources, including acetyl-CoA (genes phaA and phaB ), fatty acids (gene phaG ), and lipids (gene phaJ) [ 62 ] . Then, these monomers are polymerized into PHA by the PHA synthase (gene phaC ).…”