In silico Analysis of Pasteurella multocida PlpE Protein Epitopes As Novel Subunit Vaccine Candidates

Mostaan, Saied; Ghasemzadeh, Abbas; Ehsani, Parastoo; Sardari, Soroush; Shokrgozar, Mohammad Ali; Abolhassani, Mohsen; Brujeni, Gholamreza Nikbakht

doi:10.29252/ibj.25.1.41

Cited by 20 publications

(27 citation statements)

References 16 publications

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“…P. multocida lipoprotein E (PlpE), with a molecular weight of about 38 kDa, is an important immunogenic outer membrane protein in P. multocida (30); According to the found of Mostaan etal. (31), PlpE had immunogenicity, antigenicity, different serotypes coverage, and antibody accessibility, it was a lipid-modified surface-exposed outer membrane protein with an important role in complementmediated killing (32), and previous studies demonstrated that recombinant PlpE is protective and safety in mice, rabbits, chickens and calves (30). The PlpE gene is widely present and has a high homology among serotypes of P. multocida (32).…”

Section: Introductionmentioning

confidence: 99%

Immunogenicity and protective efficacy of the recombinant Pasteurella multocida lipoproteins VacJ and PlpE, and outer membrane protein H from P. multocida A:1 in ducks

Xiao²,

Chang³

et al. 2022

Front. Immunol.

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Duck cholera (duck hemorrhagic septicemia) is a highly contagious disease caused by Pasteurella multocida, and is one of the major bacterial diseases currently affecting the duck industry. Type A is the predominant pathogenic serotype. In this study, the genes encoding the lipoproteins VacJ, PlpE, and the outer membrane protein OmpH of P. multocida strain PMWSG-4 were cloned and expressed as proteins in E. coli. The recombinant VacJ (84.4 kDa), PlpE (94.8 kDa), and OmpH (96.7 kDa) proteins were purified, and subunit vaccines were formulated with a single water-in-oil adjuvant, while killed vaccines were prepared using a single oil-coated adjuvant. Antibody responses in ducks vaccinated with recombinant VacJ, PlpE, and OmpH proteins formulated with adjuvants were significantly antigenic (p<0.005). Protectivity of the vaccines was evaluated via the intraperitoneal challenge of ducks with 20 LD50 doses of P. multocida A: 1. The vaccine formulation consisting of rVacJ, rPlpE, rOmpH, and adjuvant provided 33.3%, 83.33%, and 83.33% protection, respectively, the vaccine formulation consisting of three recombinant proteins, rVacJ, rPlpE, rOmpH and adjuvant, was 100% protective, and the killed vaccine was 50% protective. In addition, it was shown through histopathological examination and tissue bacterial load detection that all vaccines could reduce tissue damage and bacterial colonization to varying (p<0.001). These findings indicated that recombinant PlpE or OmpH fusion proteins formulated with oil adjuvants have the potential to be used as vaccine candidates against duck cholera subunits.

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Section: Introductionmentioning

confidence: 99%

Immunogenicity and protective efficacy of the recombinant Pasteurella multocida lipoproteins VacJ and PlpE, and outer membrane protein H from P. multocida A:1 in ducks

Xiao²,

Chang³

et al. 2022

Front. Immunol.

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“…The isolated bacteria were inoculated into nutrient broth containing 5% FBS at 37 °C for 24 h and subsequently colony-forming units (CFU) were counted. An animal experiment was referenced [ 29 ], but with modifications. Six 4-week-old SPF Kunming mice were each injected intraperitoneally with 2 × 10 8 CFU of the bacteria.…”

Section: Methodsmentioning

confidence: 99%

Detection of Porcine Circovirus Type 2a and Pasteurella multocida Capsular Serotype D in Growing Pigs Suffering from Respiratory Disease

et al. 2022

Veterinary Sciences

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In order to diagnose a respiratory disease in a pig farm, the lungs, spleen, and lymph nodes of three dead pigs were collected for pathogen detection by PCR and isolation on the basis of preliminary clinical diagnosis. The virus isolate was identified by gene sequence analysis and Immunoperoxidase monolayer assay (IPMA). The bacterial isolate was identified by biochemical tests, 16S rDNA sequence analysis, and species- and serotype-specific PCR, and the pathogenicity was analyzed. Porcine circovirus type 2a (PCV2a) genotype from the lungs, spleen, and lymph nodes and Pasteurella (P.) multocida capsular serotypes D from the lungs were found. The PCV2a isolates could specifically bound the anti-PCV2-Cap polyclonal antibody. The 16S rDNA sequence of P. multocida isolates had 99.9% identity with that of the strain from cattle, and the isolate was highly pathogenic to mice. The results showed that the co-infection of PCV2a and P. Multocida capsular serotypes D should be responsible for the disease. The uncommon PCV2a is still prevalent in some pig farms besides the dominant PCV2d genotype. This study could provide important etiological information for effective control and treatment of the disease in pig farms.

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“…Although in vitro and in vivo vaccine discoveries are time-consuming and costly, besides possessing animal ethical issues, a recent in silico study has shed light on the search for serotype-independent vaccine candidates [49]. The reverse vaccinology strategy that was performed successfully elicited the most promising order of P. multocida PlpE epitopes in a multi-epitope model that can be used as a novel subunit vaccine candidate [49]. Therefore, it is important to explore continuously improvized vaccine design strategies that later require improved biomass and cell viability with effective control methods.…”

Section: Pasteurella Multocida and Mannheimia Haemolyticamentioning

confidence: 99%

Pasteurellosis Vaccine Commercialization: Physiochemical Factors for Optimum Production

et al. 2022

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Pasteurella spp. are Gram-negative facultative bacteria that cause severe economic and animal losses. Pasteurella-based vaccines are the most promising solution for controlling Pasteurella spp. outbreaks. Remarkably, insufficient biomass cultivation (low cell viability and productivity) and lack of knowledge about the cultivation process have impacted the bulk production of animal vaccines. Bioprocess optimization in the shake flask and bioreactor is required to improve process efficiency while lowering production costs. However, its state of the art is limited in providing insights on its biomass upscaling, preventing a cost-effective vaccine with mass-produced bacteria from being developed. In general, in the optimum cultivation of Pasteurella spp., production factors such as pH (6.0–8.2), agitation speed (90–500 rpm), and temperature (35–40 °C) are used to improve production yield. Hence, this review discusses the production strategy of Pasteurella and Mannheimia species that can potentially be used in the vaccines for controlling pasteurellosis. The physicochemical factors related to operational parameter process conditions from a bioprocess engineering perspective that maximize yields with minimized production cost are also covered, with the expectation of facilitating the commercialization process.

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In silico Analysis of Pasteurella multocida PlpE Protein Epitopes As Novel Subunit Vaccine Candidates

Cited by 20 publications

References 16 publications

Immunogenicity and protective efficacy of the recombinant Pasteurella multocida lipoproteins VacJ and PlpE, and outer membrane protein H from P. multocida A:1 in ducks

Immunogenicity and protective efficacy of the recombinant Pasteurella multocida lipoproteins VacJ and PlpE, and outer membrane protein H from P. multocida A:1 in ducks

Detection of Porcine Circovirus Type 2a and Pasteurella multocida Capsular Serotype D in Growing Pigs Suffering from Respiratory Disease

Pasteurellosis Vaccine Commercialization: Physiochemical Factors for Optimum Production

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