In silico analysis of essential and non-homologous proteins in Salmonella typhimurium biofilm

Abstract

Salmonella typhimurium is a Gram negative pathogen that commonly causes severe gastroenteritis. It is resistant to a wide range of antibiotics and is able to form biofilm on both biotic and abiotic surfaces. To date, essential and non-homologous proteins in S. typhimurium biofilm remain not well investigated. Therefore, the present work was performed to analyze essential and non-homologous proteins in S. typhimurium biofilm using a combination of one-dimensional SDS-PAGE, HPLC - ESI - QTOF and bioinformatics. Results demonstrated that seven major protein bands (78.1 kDa, 51.2 kDa, 41.5 kDa, 37.3 kDa, 35.1 kDa, 27.6 kDa, and 25.4 kDa) were present in whole-cell protein extract of S. typhimurium biofilm. A total of 75 proteins were successfully identified from both 25.4 kDa and 51.2 kDa protein bands. Approximately 54.67% of QTOF-identified whole-cell proteins were found to be essential to the survival of S. typhimurium biofilm and were non-homologous to human proteome. Majority of essential and non-homologous S. typhimurium biofilm proteins were associated with transport and protein synthesis. The findings from the present work may be useful for development of novel antibiofilm agent.

Methanolic Extract Of Swietenia macrophylla Exhibits Antibacterial And Antibiofilm Efficacy Against Gram-Positive Pathogens

“…Biofilm refers to a sessile, densely packed, and chemically heterogeneous microbial community (Yaacob et al, 2021a). It is known to express multiple essential proteins underlying complex protein interaction networks (Othman & Yahya 2019;Yaacob et al, 2021b). Further investigations of existing natural products, antifungals, and antibiotics are needed to better fight against a wide spectrum of biofilm infections (Johari et al, 2020;Isa et al, 2022;Rashid et al, 2022).…”

Biofilm-associated proteins as potential drug targets

“…Understanding the role of BAPs is crucial in developing effective strategies to target biofilms as potential drug targets. These proteins possess various characteristics, including essential metabolic functions within biofilms and the distinction of being non-host homologous (Othman and Yahya, 2019). Although many antibiotics, disinfectants, antifungals and natural products have been demonstrated to possess antibiofilm potential, their effects on expression of the BAPs remain not well investigated (Isa et al, 2022;Kamaruzzaman et al, 2022;Amran et al, 2023;Johari et al, 2023).…”

FTIR Spectroscopic Study Of Inhibition of Chloroxylenol-Based Disinfectant Against Salmonella enterica serovar Thyphimurium Biofilm

“…Most bacteria can form biofilms including Salmonella. Salmonella biofilms can adhere to surfaces such as stainless steel, polyester, plastic, and aluminum (Alves et al, 2015;Merino et al, 2019;Othman & Yahya 2019;Tassinari et al, 2019). Biofilms are a group of microbial cells that are adhered to a living or inert surface and encased themselves in a self-produced extracellular polymeric matrix (Mahat et al, 2012;Yaacob et al, 2021).…”