In-silico analysis and homology modelling of antioxidant proteins present in Pisum sativum

Abstract

Garden Peas are edible seeds that have an essential nutrient required for human diet. Peas contain p hytochemicals t hat s hows a ntioxidant activities. A computational approach was adopted to analyse the characteristic properties and structure of the Peas antioxidant proteins. Superoxidase dismutase (SOD), catalase, phospholipid hydroperoxide g lutathione p eroxidase-like p rotein (PHGPX) and ascorbate peroxidase (APX) are the antioxidant proteins which have been used in this study. Physiochemical properties such as isoelectric point, molecular weight, aliphatic index etc. were analysed by using the Expasy's Prot Param server. Secondary structure of these proteins was analysed to study the functional characteristic of the protein. The crystal structure of the ascorbate peroxidase was available, but the crystal structures of other proteins were not available in any of the protein structure database. Hence the threedimensional structures of these proteins were generated using Swiss Modeller and Geno3D followed by validation through SAVES server. This analysis will provide additional help for the analysis of crystal structure for further experimentation.