In silico Analyses of Transcriptomes of the Marine Green Microalga Dunaliella tertiolecta: Identification of Sequences Encoding P-type ATPases

Попова, Л. Г.; Belyaev, D. V.; Shuvalov, Alexey; Yurchenko, Andrey A.; Matalin, D. A.; Khramov, Dmitrii E.; Orlova, Yu. V.; Balnokin, Yu. V.

doi:10.1134/s0026893318040167

Cited by 8 publications

(8 citation statements)

References 45 publications

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“…While Popova et al . (2018) could not find a P-type Na + -ATPase in the genome of Dunaliella tertiolecta , Popova et al . (2005; see also Shumkova et al ., 2000) functionally identified an electrogenic Na + -translocating ATPase in Dunaliella maritima using inside-out plasmalemma vesicles (see also Popova & Balnokin, 2013).…”

Section: Organisms With Chlorophyllsmentioning

confidence: 99%

“…The Chlorophyta: Chlorophyceae are mainly freshwater but with some marine representatives (Tragin & Vaulot, 2018; Del Cortona et al ., 2020), the P-type ATPases of the genus Dunaliella has been the subject of considerable investigation (Wolf et al ., 1995; Weiss & Pick, 1996; Popova et al ., 2018). Sequences encoding P-type H + -ATPases have been found in the genomes of the halophilic Dunaliella bioculata (Smahel et al ., 1990; Wolf et al ., 1995), Dunaliella salina (Weiss & Pick, 1996; Katz et al ., 2007), Dunaliella bioculata (Bertucci et al ., 2010) and Dunaliella tertiolecta (Popova et al ., 2018), as well as the acidophilic Dunaliella acidophila (Weiss & Pick, 1996; Bertucci et al ., 2010). While Popova et al .…”

Section: Organisms With Chlorophyllsmentioning

confidence: 99%

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Energizing the plasmalemma of marine photosynthetic organisms: the role of primary active transport

Raven

Beardall

2020

J. Mar. Biol. Ass.

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Generation of ion electrochemical potential differences by primary active transport can involve energy inputs from light, from exergonic redox reactions and from exergonic ATP hydrolysis. These electrochemical potential differences are important for homoeostasis, for signalling, and for energizing nutrient influx. The three main ions involved are H+, Na+ (efflux) and Cl− (influx). In prokaryotes, fluxes of all three of these ions are energized by ion-pumping rhodopsins, with one archaeal rhodopsin pumping H+into the cells; among eukaryotes there is also an H+ influx rhodopsin in Acetabularia and (probably) H+ efflux in diatoms. Bacteriochlorophyll-based photoreactions export H+ from the cytosol in some anoxygenic photosynthetic bacteria, but chlorophyll-based photoreactions in marine cyanobacteria do not lead to export of H+. Exergonic redox reactions export H+ and Na+ in photosynthetic bacteria, and possibly H+ in eukaryotic algae. P-type H+- and/or Na+-ATPases occur in almost all of the photosynthetic marine organisms examined. P-type H+-efflux ATPases occur in charophycean marine algae and flowering plants whereas P-type Na+-ATPases predominate in other marine green algae and non-green algae, possibly with H+-ATPases in some cases. An F-type Cl−-ATPase is known to occur in Acetabularia. Some assignments, on the basis of genomic evidence, of P-type ATPases to H+ or Na+ as the pumped ion are inconclusive.

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Section: Organisms With Chlorophyllsmentioning

confidence: 99%

Section: Organisms With Chlorophyllsmentioning

confidence: 99%

Energizing the plasmalemma of marine photosynthetic organisms: the role of primary active transport

Raven

Beardall

2020

J. Mar. Biol. Ass.

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“…In order to identify the gene of Na + -ATPase for algae belonging to the genus Dunaliella we assembled de novo several transcriptomes of the microalga D. tertiolecta based on individual libraries of short RNA reads from D. tertiolecta available in free access in the Sequence Read Archive (SRA, NCBI) database. The assembled transcriptomes were examined in silico for possible signatures for P-type ATPases [56]. Coding sequences (CDS) for various P-type ATPases were found, but contrary to expectations, none of the assembled D. tertiolecta transcriptomes demonstrated a nucleotide sequence encoding a protein that could be unambiguously assigned to the Na + -ATPase group.…”

Section: Introductionmentioning

confidence: 75%

“…The other putative D. tertiolecta ATPase, DtHA2, was similar (99% identical amino acid residues) to P-type ATPase of the halotolerant microalga D. salina (ABB88698.1, GenBank database). The phylogenetic analysis demonstrated that ATPase DtHA1 is homologous to the proton pumps of higher plants, while ATPase DtHA2 is homologous to H + -ATPases of microalgae and parasitic protists [56].…”

Section: Introductionmentioning

confidence: 99%

Cloning and Characterization of Two Putative P-Type ATPases from the Marine Microalga Dunaliella maritima Similar to Plant H+-ATPases and Their Gene Expression Analysis under Conditions of Hyperosmotic Salt Shock

Matalin

Khramov

Shuvalov

et al. 2021

Plants

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The green microalga genus Dunaliella is mostly comprised of species that exhibit a wide range of salinity tolerance, including inhabitants of hyperhaline reservoirs. Na+ content in Dunaliella cells inhabiting saline environments is maintained at a fairly low level, comparable to that in the cells of freshwater organisms. However, despite a long history of studying the physiological and molecular mechanisms that ensure the ability of halotolerant Dunaliella species to survive at high concentrations of NaCl, the question of how Dunaliella cells remove excess Na+ ions entering from the environment is still debatable. For thermodynamic reasons it should be a primary active mechanism; for example, via a Na+-transporting ATPase, but the molecular identification of Na+-transporting mechanism in Dunaliella has not yet been carried out. Formerly, in the euryhaline alga D. maritima, we functionally identified Na+-transporting P-type ATPase in experiments with plasma membrane (PM) vesicles which were isolated from this alga. Here we describe the cloning of two putative P-type ATPases from D. maritima, DmHA1 and DmHA2. Phylogenetic analysis showed that both ATPases belong to the clade of proton P-type ATPases, but the similarity between DmHA1 and DmHA2 is not high. The expression of DmHA1 and DmHA2 in D. maritima cells under hyperosmotic salt shock was studied by qRT-PCR. Expression of DmHA1 gene decreases and remains at a relatively low level during the response of D. maritima cells to hyperosmotic salt shock. In contrast, expression of DmHA2 increases under hyperosmotic salt shock. This indicates that DmHA2 is important for overcoming hyperosmotic salt stress by the algal cells and as an ATPase it is likely directly involved in transport of Na+ ions. We assume that it is the DmHA2 ATPase that represents the Na+-transporting ATPase.

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“…The activities of Na + -P-ATPase have been identified for various algal species, including Tetraselmis viridis (Prasinophyceae) [ 38 , 39 ] and Dunaliella parva [ 40 ]. There was an early identification of the Na + -ATPase gene from Heterosigma akashiwo [ 41 , 42 ] and the more recent discovery of genes encoding Na + -ATPases in red and green algae Chondrus crispus , Chlamydomonas reinhardtii , Ostreococcus tauri , and others are very similar to animal Na + /K + -P-ATPases [ 31 , 43 , 44 , 45 , 46 ]. The EST (Expressed Sequence Tags) analysis of Porphyra yezoensis gametophytes and sporophytes revealed the presence of Na + /K + -P-ATPase (PyKPA1) similar to that in animals [ 47 ].…”

Section: Introductionmentioning

confidence: 99%

Evolution of Plant Na+-P-Type ATPases: From Saline Environments to Land Colonization

Dabravolski

Isayenkov

2021

Plants

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Soil salinity is one of the major factors obstructing the growth and development of agricultural crops. Eukaryotes have two main transport systems involved in active Na+ removal: cation/H+ antiporters and Na+-P-type ATPases. Key transport proteins, Na+/K+-P-ATPases, are widely distributed among the different taxa families of pumps which are responsible for keeping cytosolic Na+ concentrations below toxic levels. Na+/K+-P-ATPases are considered to be absent in flowering plants. The data presented here are a complete inventory of P-type Na+/K+-P-ATPases in the major branches of the plant kingdom. We also attempt to elucidate the evolution of these important membrane pumps in plants in comparison with other organisms. We were able to observe the gradual replacement of the Na+-binding site to the Ca2+-binding site, starting with cyanobacteria and moving to modern land plants. Our results show that the α-subunit likely evolved from one common ancestor to bacteria, fungi, plants, and mammals, whereas the β-subunit did not evolve in green algae. In conclusion, our results strongly suggest the significant differences in the domain architecture and subunit composition of plant Na+/K+-P-ATPases depending on plant taxa and the salinity of the environment. The obtained data clarified and broadened the current views on the evolution of Na+/K+-P-ATPases. The results of this work would be helpful for further research on P-type ATPase functionality and physiological roles.

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In silico Analyses of Transcriptomes of the Marine Green Microalga Dunaliella tertiolecta: Identification of Sequences Encoding P-type ATPases

Cited by 8 publications

References 45 publications

Energizing the plasmalemma of marine photosynthetic organisms: the role of primary active transport

Energizing the plasmalemma of marine photosynthetic organisms: the role of primary active transport

Cloning and Characterization of Two Putative P-Type ATPases from the Marine Microalga Dunaliella maritima Similar to Plant H+-ATPases and Their Gene Expression Analysis under Conditions of Hyperosmotic Salt Shock

Evolution of Plant Na+-P-Type ATPases: From Saline Environments to Land Colonization

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