In sacco rumen disappearance of condensed tannins, fiber, and nitrogen from herbaceous native Texas legumes in goats

Pawelek, D.L.; Muir, James P.; Lambert, B.D.; Wittie, Roger

doi:10.1016/j.anifeedsci.2007.02.014

Cited by 14 publications

(16 citation statements)

References 23 publications

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“…However, the obtained results for insoluble fraction in consistent with Coblentz [6] (45.9) and pawelek [29] (43%). Andrighetto et al [2] showed the values of soluble and insoluble fraction for DM of alfalfa about 17.9 and 45.1%, respectively that is consistent with the obtained data in this experiment.…”

Section: Resultssupporting

confidence: 80%

Determining Nutritive Values of Alfalfa Cuts Using in situ and Gas Production Techniques

Taghizadeh¹

2008

American Journal of Animal and Veterinary Sciences

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Abstract:In order to determine of nutritive value of alfalfa in different cuts using in situ and gas production technique, this study was carried out. Three wethers (49±2.6 kg) were used in in situ method. The gas production was measured at 2, 4,6,8,12,16,24,36,48,72 and 96 h and ruminal dry matter and crude protein disappearance were measured at 0,4,8,12,16,24,36,48,72 and 96 h. Dry matter degradability's in first, second and third cuts of alfalfa at 96 h were 60.47, 64.71 and 64.36%, respectively. Crude protein degradability's of mentioned cuts were 60.47, 63.08 and 58.07%, respectively. The gas productions of them at 96 h were 322.54, 295.21 and 300.32 mL g −1 DM respectively. The relationship between dry matter and gas production values for alfalfa cuts obtained about 0.89, 0.85 and 0.84 and for crude protein and gas production data achieved 0.87, 0.88 and 0.84, respectively. High correlation between in situ and gas production techniques indicated that in situ degradability's values can be predicted from gas production data.

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Section: Resultssupporting

confidence: 80%

Determining Nutritive Values of Alfalfa Cuts Using in situ and Gas Production Techniques

Taghizadeh¹

2008

American Journal of Animal and Veterinary Sciences

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“…Protein-binding affinity affects ruminant nutrition since it prevents microbial degradation in the rumen (Silanikove et al 2001;Min and Hart 2003;Pawelek et al 2008). PTC PB declined following 100% defoliation in this study as well as in that reported by Cooper et al (2014) corresponding to the physiologically costly requirements of making protein-binding compounds (Coley et al 1985;Keinänen et al 1999), since polyphenolic production is reliant on photosynthesis to fix carbon (Bryant et al 1983;McDonald et al 1999).…”

Section: Discussionmentioning

confidence: 56%

Effects of simulated and insect herbivory on nitrogen and protein precipitable phenolic concentrations of two legumes

Blackmon

2016

Journal of Plant Interactions

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Protein-precipitating polyphenolics (PPPs) serve as a plant defense against herbivory, increasing with stress. We studied how varying intensities of simulated and Melanoplus differentialis herbivory affected (1) PPP concentration; (2) protein bound by PPP (PB); and (3) N concentration of panicled tick-clover (Desmodium paniculatum; PTC) and sericea lespedeza (Lespedeza cuneata; SL) leaf regrowth. Leaves of PTC that were submitted to simulated herbivory had lesser (p ≤ .05 for all significant differences) PPP concentration than the control for most treatments. For PTC, PPP concentration decreased with increasing herbivory intensity for both herbivory types. For SL, PPP was similar between herbivory types for Harvest 1 but not for 2, decreasing as herbivory intensity increased for both herbivory types. Simulated herbivory resulted in lower PB concentrations for PTC and SL compared to the grasshopper herbivory. Nitrogen concentration was similar for PTC and SL between herbivory types but variable among degree of herbivory. Herbivory type affects PPP. ARTICLE HISTORY

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“…Based on the methods described by rskov and McDonald (1979) and Pawelek et al (2008), protein breakdown was measured in situ in the same substrates. Dacron bags 5x12.5 cm and 50 ±15m pore size (AnKOM Technology, USA; bL101805)) were fitted in two rumen fistulated cows containing samples of 500 mg of CP from each feed to be tested.…”

Section: Test 3 Protein Breakdown Through the In Situ Methodsmentioning

confidence: 99%

In vitro protein breakdown by enzyme extracts of rumen origin: comparison with methods in situ and proteases of Streptomyces griseus

Velásquez

Pichard

2010

Cienc. Inv. Agr.

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A. Velásquez, and G. Pichard. 2010. In vitro protein breakdown by enzyme extracts of rumen origin: comparison with methods in situ and proteases of Streptomyces griseus. Cien. Inv. Agr. 37(3): 57-70. Proteolytic activity of enzymatic extracts generated from rumen microorganisms cultivated in vitro was evaluated. The incubation of rumen fluid used different substrates to generate a higher enzyme concentration and promote a broad spectrum of hydrolytic activity. The composition of the substrates used in the cultivation of the fluid was enriched in protein, starch or cell wall. Enzyme preparations were evaluated by incubating in 30 mL of buffer 50 mM Tris-HCl (pH 6.5) at 39 ºC during 48 hours, 100 mg of crude protein from feeds soybean meal, canola meal, sunflower meal, gluten feed, dehydrated alfalfa meal, berseem clover, oat forage and perennial ryegrass. Enzyme extracts from cultivated rumen fluid showed an average protein breakdown of 75.5%, in eight feed samples tested. This value was very close to that measured with the technique of proteases from Streptomyces griseus (74.6% CP), but significantly lower (P≤0.05) than the one obtained by the in situ methodology (84.8% CP). The technique with extracted rumen enzymes showed higher level of proteolysis in the early hours of incubation (6 H) compared to the other techniques. These results suggest that the enzyme preparations of ruminal origin have the ability to predict degradability of feed proteins in the rumen, particularly in the first phase when most of proteins are hydrolyzed and become available for microbial utilization.

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In sacco rumen disappearance of condensed tannins, fiber, and nitrogen from herbaceous native Texas legumes in goats

Cited by 14 publications

References 23 publications

Determining Nutritive Values of Alfalfa Cuts Using in situ and Gas Production Techniques

Determining Nutritive Values of Alfalfa Cuts Using in situ and Gas Production Techniques

Effects of simulated and insect herbivory on nitrogen and protein precipitable phenolic concentrations of two legumes

In vitro protein breakdown by enzyme extracts of rumen origin: comparison with methods in situ and proteases of Streptomyces griseus

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