In primary airway epithelial cells, the unjamming transition is distinct from the epithelial-to-mesenchymal transition

Mitchel, Joseph F.; Das, Amit; O’Sullivan, Michael J.; Stancil, I.T.; DeCamp, Stephen J.; Koehler, Stephan A.; Ocaña, Oscar H.; Butler, James P.; Fredberg, Jeffrey J.; Nieto, M. Ángela; Bi, Dapeng; Park, Jin‐Ah

doi:10.1038/s41467-020-18841-7

Cited by 143 publications

(208 citation statements)

References 155 publications

(306 reference statements)

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“…This suggests that cell jamming 18,29,61,64 cannot be merely characterized by a dynamical slow down, but rather requires at least one additional dynamical parameter to characterize the degree of cooperativity. These results are in agreement with recent work showing that human airway epithelial cells can also undergo solid-to-fluid transitions via different dynamical and morphological paths 65 .…”

Section: Discussionsupporting

confidence: 93%

Energetics of mesoscale cell turbulence in two-dimensional monolayers

Lin

Zhang

et al. 2021

Commun Phys

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Investigation of energy mechanisms at the collective cell scale is a challenge for understanding various biological processes, such as embryonic development and tumor metastasis. Here we investigate the energetics of self-sustained mesoscale turbulence in confluent two-dimensional (2D) cell monolayers. We find that the kinetic energy and enstrophy of collective cell flows in both epithelial and non-epithelial cell monolayers collapse to a family of probability density functions, which follow the q-Gaussian distribution rather than the Maxwell–Boltzmann distribution. The enstrophy scales linearly with the kinetic energy as the monolayer matures. The energy spectra exhibit a power-decaying law at large wavenumbers, with a scaling exponent markedly different from that in the classical 2D Kolmogorov–Kraichnan turbulence. These energetic features are demonstrated to be common for all cell types on various substrates with a wide range of stiffness. This study provides unique clues to understand active natures of cell population and tissues.

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Section: Discussionsupporting

confidence: 93%

Energetics of mesoscale cell turbulence in two-dimensional monolayers

Lin

Zhang

et al. 2021

Commun Phys

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“…To support this idea, here we discussed recent evidence indicating that bronchoconstriction facilitates the pathologic process through the activation of the airway epithelial cells. With the evidence from multiple approaches, including transcriptional and translational regulation, signalling pathways, RNA-sequencing analysis and collective cellular migration, it is evident that mechanically compressed airway epithelial cells play a causal role in the pathophysiology of asthma [13][14][15][16][17]35,38,39,61]. Competing interests.…”

Section: Resultsmentioning

confidence: 99%

“…10: 200254 the epithelial-to-mesenchymal transition (EMT) through the activation of the TGF-β receptor. However, signs of EMT were not observed during the UJT in HBE cells [59,61].…”

Section: Airway Epithelial Unjamming By Mechanical Compressionmentioning

confidence: 91%

“…Furthermore, the biochemical and biophysical characteristics of the cellular migration between the EMT and UJT are distinct [61]. royalsocietypublishing.org/journal/rsob Open Biol.…”

Section: Airway Epithelial Unjamming By Mechanical Compressionmentioning

confidence: 99%

“…One could hypothesize that the increased cellular motility during the UJT is due to the epithelial-to-mesenchymal transition (EMT) through the activation of the TGF-β receptor. However, signs of EMT were not observed during the UJT in HBE cells [ 59 , 61 ]. Furthermore, the biochemical and biophysical characteristics of the cellular migration between the EMT and UJT are distinct [ 61 ].…”

Section: Airway Epithelial Cells During Bronchoconstrictionmentioning

confidence: 99%

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Bronchoconstriction: a potential missing link in airway remodelling

2020

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In asthma, progressive structural changes of the airway wall are collectively termed airway remodelling. Despite its deleterious effect on lung function, airway remodelling is incompletely understood. As one of the important causes leading to airway remodelling, here we discuss the significance of mechanical forces that are produced in the narrowed airway during asthma exacerbation, as a driving force of airway remodelling. We cover in vitro , ex vivo and in vivo work in this field, and discuss up-to-date literature supporting the idea that bronchoconstriction may be the missing link in a comprehensive understanding of airway remodelling in asthma.

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Tissue Engineering with Mechanically Induced Solid‐Fluid Transitions

et al. 2021

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Epithelia are contiguous sheets of cells that stabilize the shape of internal organs and support their structure by covering their surfaces. They acquire diverse morphological forms appropriate for their specific functions during embryonic development, such as the kidney tubules and the complex branching structures found in the lung. The maintenance of epithelial morphogenesis and homeostasis is controlled by their remarkable mechanics—epithelia can become elastic, plastic, and viscous by actively remodeling cell–cell junctions and modulating the distribution of local stresses. Microfabrication, finite element modelling, light‐sheet microscopy, and robotic micromanipulation are used to show that collagen gels covered with an epithelial skin serve as shape‐programmable soft matter. The process involves solid to fluid transitions induced by mechanical perturbations, generates spatially distributed surface stresses at tissue interfaces, and is amenable to both additive and subtractive manufacturing techniques. The robustness and versatility of this strategy for engineering designer tissues is demonstrated by directing the morphogenesis of a variety of molded, carved, and assembled forms from the base material. The results provide insight into the active mechanical properties of the epithelia and establish methods for engineering tissues with sustainable architectures.

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In primary airway epithelial cells, the unjamming transition is distinct from the epithelial-to-mesenchymal transition

Cited by 143 publications

References 155 publications

Energetics of mesoscale cell turbulence in two-dimensional monolayers

Energetics of mesoscale cell turbulence in two-dimensional monolayers

Bronchoconstriction: a potential missing link in airway remodelling

Tissue Engineering with Mechanically Induced Solid‐Fluid Transitions

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