In planta induced changes in the native plasmid profile of Pseudomonas syringae pathover phaseolicola strain 1302A

Neale, Helen C.; Slater, Ross T.; Mayne, Lisa-Marie; Manoharan, Bharani; Arnold, Dawn L.

doi:10.1016/j.plasmid.2013.07.002

Cited by 9 publications

(9 citation statements)

References 15 publications

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“…maculicola via recombination between alleles of the T3SS effector avrpPhE [31], and the PFP pAV505 of P. syringae pv. phaseolicola 1302A and two other native plasmids were presumably integrated into the chromosome of the host strain after passage through a resistant bean cultivar [59]. In many cases, these intragenome plasmid recombination or excision events appear to occur as a consequence of host recognition/avoidance scenarios [60].…”

Section: Discussionmentioning

confidence: 99%

Complete sequence and comparative genomic analysis of eight native Pseudomonas syringae plasmids belonging to the pPT23A family

Gutiérrez‐Barranquero

Cazorla

Vicente

et al. 2017

BMC Genomics

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BackgroundThe pPT23A family of plasmids appears to be indigenous to the plant pathogen Pseudomonas syringae and these plasmids are widely distributed and widely transferred among pathovars of P. syringae and related species. pPT23A-family plasmids (PFPs) are sources of accessory genes for their hosts that can include genes important for virulence and epiphytic colonization of plant leaf surfaces. The occurrence of repeated sequences including duplicated insertion sequences on PFPs has made obtaining closed plasmid genome sequences difficult. Therefore, our objective was to obtain complete genome sequences from PFPs from divergent P. syringae pathovars and also from strains of P. syringae pv. syringae isolated from different hosts.ResultsThe eight plasmids sequenced ranged in length from 61.6 to 73.8 kb and encoded from 65 to 83 annotated orfs. Virulence genes including type III secretion system effectors were encoded on two plasmids, and one of these, pPt0893-29 from P. syringae pv. tabaci, encoded a wide variety of putative virulence determinants. The PFPs from P. syringae pv. syringae mostly encoded genes of importance to ecological fitness including the rulAB determinant conferring tolerance to ultraviolet radiation. Heavy metal resistance genes encoding resistance to copper and arsenic were also present in a few plasmids. The discovery of part of the chromosomal genomic island GI6 from P. syringae pv. syringae B728a in two PFPs from two P. syringae pv. syringae hosts is further evidence of past intergenetic transfers between plasmid and chromosomal DNA. Phylogenetic analyses also revealed new subgroups of the pPT23A plasmid family and confirmed that plasmid phylogeny is incongruent with P. syringae pathovar or host of isolation. In addition, conserved genes among seven sequenced plasmids within the same phylogenetic group were limited to plasmid-specific functions including maintenance and transfer functions.ConclusionsOur sequence analysis further revealed that PFPs from P. syringae encode suites of accessory genes that are selected at species (universal distribution), pathovar (interpathovar distribution), and population levels (intrapathovar distribution). The conservation of type IV secretion systems encoding conjugation functions also presumably contributes to the distribution of these plasmids within P. syringae populations.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-017-3763-x) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Complete sequence and comparative genomic analysis of eight native Pseudomonas syringae plasmids belonging to the pPT23A family

Gutiérrez‐Barranquero

Cazorla

Vicente

et al. 2017

BMC Genomics

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“…Indeed, the loss and gain of effector-containing plasmids and islands can be observed after a few in planta passages [17]. This rampant exchange of genetic material can result in chimeric genomes, as exemplified by Xanthomonas species, which contain genes from groups as disparate as the Archaea and Eukarya [18].…”

Section: Multiple Processes Maintain Genomic Variationmentioning

confidence: 99%

Genomic variability as a driver of plant–pathogen coevolution?

Karasov

Horton

Bergelson

2014

Current Opinion in Plant Biology

126

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Pathogens apply one of the strongest selective pressures in plant populations. Understanding plant–pathogen coevolution has therefore been a major research focus for at least sixty years [1]. Recent comparative genomic studies have revealed that the genes involved in plant defense and pathogen virulence are among the most polymorphic in the respective genomes. Which fraction of this diversity influences the host–pathogen interaction? Do coevolutionary dynamics maintain variation? Here we review recent literature on the evolutionary and molecular processes that shape this variation, focusing primarily on gene-for-gene interactions. In summarizing theoretical and empirical studies of the processes that shape this variation in natural plant and pathogen populations, we find a disconnect between the complexity of ecological interactions involving hosts and their myriad microbes, and the models that describe them.

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“…Genotypic diversity has been shown to arise during plant colonization by Pseudomonas syringae. During infection by an initially homogeneous population, genetic changes generated through mutation or chromosomal re-organization within bacteria under stress can give rise to bacterial variants with phenotypic differences that impact on their interactions with the host (Lovell et al, 2009Neale et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Confocal microscopy reveals in planta dynamic interactions between pathogenic, avirulent and non‐pathogenic Pseudomonas syringae strains

Rufián

Macho

Corry

et al. 2017

Molecular Plant Pathology

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Recent advances in genomics and single-cell analysis have demonstrated the extraordinary complexity reached by microbial populations within their hosts. Communities range from complex multispecies groups to homogeneous populations differentiating into lineages through genetic or non-genetic mechanisms. Diversity within bacterial populations is recognized as a key driver of the evolution of animal pathogens. In plants, however, little is known about how interactions between different pathogenic and non-pathogenic variants within the host impact on defence responses, or how the presence within a mixture may affect the development or the fate of each variant. Using confocal fluorescence microscopy, we analysed the colonization of the plant apoplast by individual virulence variants of Pseudomonas syringae within mixed populations. We found that non-pathogenic variants can proliferate and even spread beyond the inoculated area to neighbouring tissues when in close proximity to pathogenic bacteria. The high bacterial concentrations reached at natural entry points promote such interactions during the infection process. We also found that a diversity of interactions take place at a cellular level between virulent and avirulent variants, ranging from dominant negative effects on proliferation of virulent bacteria to in trans suppression of defences triggered by avirulent bacteria. Our results illustrate the spatial dynamics and complexity of the interactions found within mixed infections, and their potential impact on pathogen evolution.

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In planta induced changes in the native plasmid profile of Pseudomonas syringae pathover phaseolicola strain 1302A

Cited by 9 publications

References 15 publications

Complete sequence and comparative genomic analysis of eight native Pseudomonas syringae plasmids belonging to the pPT23A family

Complete sequence and comparative genomic analysis of eight native Pseudomonas syringae plasmids belonging to the pPT23A family

Genomic variability as a driver of plant–pathogen coevolution?

Confocal microscopy reveals in planta dynamic interactions between pathogenic, avirulent and non‐pathogenic Pseudomonas syringae strains

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