In planta biocontrol of soilborne Fusarium wilt of banana through a plant endophytic bacterium, Burkholderia cenocepacia 869T2

Ho, Ying-Ning; Chiang, Hsing-Mei; Chao, Chih-Ping; Su, Ching-Chung; Hsu, Hui-Fang; Guo, Chen-tong; Hsieh, Ju-Liang; Huang, Chieh-Chen

doi:10.1007/s11104-014-2297-0

Cited by 68 publications

(48 citation statements)

References 39 publications

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“…Moreover, we found that consecutive monoculture of P. heterophylla led to a significant increase in the relative abundance of F. oxysporum but a decrease in the potential beneficial bacteria including Burkholderia and Clostridium (Mowlick et al, 2012; Ho et al, 2015; Wen et al, 2016). Our previous studies by culture-dependent and culture-independent analysis demonstrated that F. oxysporum and other Fusarium spp.…”

Section: Discussionmentioning

confidence: 86%

“…A growing number of Burkholderia species have been reported to be beneficial for plants, including biological control of fungal diseases, promotion of plant growth and induction of systemic resistance (Compant et al, 2008; Ho et al, 2015). The Burkholderia strains used for solid fermentation in this study proved to have a strong antagonistic activity against F. oxysporum (Supplementary Figure S1), the main agent causing replant disease of P. heterophylla (Wu L. et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

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Insights into the Regulation of Rhizosphere Bacterial Communities by Application of Bio-organic Fertilizer in Pseudostellaria heterophylla Monoculture Regime

Chen

et al. 2016

Front. Microbiol.

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The biomass and quality of Pseudostellariae heterophylla suffers a significant decline under monoculture. Since rhizosphere miobiome plays crucial roles in soil health, deep pyrosequencing combined with qPCR was applied to characterize the composition and structure of soil bacterial community under monoculture and different amendments. The results showed compared with the 1st-year planted (FP), 2nd-year monoculture of P. heterophylla (SP) led to a significant decline in yield and resulted in a significant increase in Fusarium oxysporum but a decline in Burkholderia spp. Bio-organic fertilizer (MT) formulated by combining antagonistic bacteria with organic matter could significantly promote the yield by regulating rhizosphere bacterial community. However, organic fertilizer (MO) without antagonistic bacteria could not suppress Fusarium wilt. Multivariate statistics analysis showed a distinct separation between the healthy samples (FP and MT) and the unhealthy samples (SP and MO), suggesting a strong relationship between soil microbial community and plant performance. Furthermore, we found the application of bio-organic fertilizer MT could significantly increase the bacterial community diversity and restructure microbial community with relatively fewer pathogenic F. oxysporum and more beneficial Burkholderia spp. In conclusion, the application of novel bio-organic fertilizer could effectively suppress Fusarium wilt by enriching the antagonistic bacteria and enhancing the bacterial diversity.

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Section: Discussionmentioning

confidence: 86%

Section: Discussionmentioning

confidence: 99%

Insights into the Regulation of Rhizosphere Bacterial Communities by Application of Bio-organic Fertilizer in Pseudostellaria heterophylla Monoculture Regime

Chen

et al. 2016

Front. Microbiol.

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“…; Ho et al . ). Previous research on ginseng endophytes mostly focused on the biological diversity and the major bioactive secondary metabolites.…”

Section: Introductionmentioning

confidence: 97%

Biotransformation of ginsenoside Rb1 to ginsenoside Rg3 by endophytic bacterium Burkholderia sp. GE 17-7 isolated from Panax ginseng

Yin

2017

J Appl Microbiol

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“…However, owing to the long cultivation cycle of banana and the rapid evolution of Foc , few resistant cultivars have been used in the field to date (Ploetz, 2015). Considerable interest is therefore focused on the exploitation of naturally occurring organisms, such as nonpathogenic F. oxysporum, Burkholderia cenocepacia, Bacillus amyloliquefaciens , and Streptomyces albospinus for the control of crop diseases including FWB (Postma and Rattink, 1992; Raguchander et al, 1997; Butt et al, 2001; Fravel et al, 2003; Cao et al, 2005; Asha et al, 2011a,b; Wang et al, 2013; Ho et al, 2015). Furthermore, many antifungal secondary metabolites have been identified from plants and microorganism (Paiva et al, 2010; Coleman et al, 2011), and the identification of novel antifungal targets for use as control agents is currently becoming an important strategy (De Backer and Van Dijck, 2003; Walsh et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

RNA Sequencing Reveals that Endoplasmic Reticulum Stress and Disruption of Membrane Integrity Underlie Dimethyl Trisulfide Toxicity against Fusarium oxysporum f. sp. cubense Tropical Race 4

et al. 2017

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Fusarium wilt of banana, a destructive disease that affects banana production, is caused by Fusarium oxysporum f. sp. cubense tropical race 4 (Foc TR4). In a previous study, we confirmed the strong inhibitory effects of Chinese leek (Allium tuberosum) on the incidence of this disease. Sulfur compounds are the primary antifungal constituents of Chinese leek. Among these, dimethyl trisulfide (DT) was the most abundant and exhibited the strongest inhibition of Foc TR4 growth and development. In the present study, the global gene expression profiles of Foc TR4 isolates treated with DT at 4,000-folds dilution (concentration of 1/4,000, v/v) for 1.5, 6, and 12 h were investigated by using RNA sequencing. The expression patterns of 15 DEGs were validated based on quantitative real-time PCR (qRT-PCR) assay. Untreated sample presented 2,556, 1,691, and 1,150 differentially expressed genes (DEGs) at 1.5, 6, and 12 h after the onset of the experiment, respectively, whereas DT-treated isolates presented 2,823, 3,546, and 6,197 DEGs. Based on Gene Ontology (GO) annotation and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis, DEGs involved in endoplasmic reticulum (ER), glycosylation, and steroid biosynthesis were significantly inhibited by DT exposure. The similar expressional patterns of 15 DEGs between RNA-seq and qRT-PCR assays indicated the reliability of the RNA-seq data. In conclusion, ER stress related to glycosylation inhibition and damage to cell membrane integrity might contribute to the toxicity of DT against Foc TR4. As the results presented here evidenced changes in gene expression associated with DT exposure, which might be used to develop new approaches for controlling FWB.

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In planta biocontrol of soilborne Fusarium wilt of banana through a plant endophytic bacterium, Burkholderia cenocepacia 869T2

Cited by 68 publications

References 39 publications

Insights into the Regulation of Rhizosphere Bacterial Communities by Application of Bio-organic Fertilizer in Pseudostellaria heterophylla Monoculture Regime

Insights into the Regulation of Rhizosphere Bacterial Communities by Application of Bio-organic Fertilizer in Pseudostellaria heterophylla Monoculture Regime

Biotransformation of ginsenoside Rb1 to ginsenoside Rg3 by endophytic bacterium Burkholderia sp. GE 17-7 isolated from Panax ginseng

RNA Sequencing Reveals that Endoplasmic Reticulum Stress and Disruption of Membrane Integrity Underlie Dimethyl Trisulfide Toxicity against Fusarium oxysporum f. sp. cubense Tropical Race 4

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