In ovo protective effects of chicoric and rosmarinic acids against Thiacloprid-induced cytotoxicity, oxidative stress, and growth retardation on newly hatched chicks

Farag, Mayada R.; Zizzadoro, Claudia; Alagawany, Mahmoud; Abou‐Zeid, Shimaa M.; Mawed, Suzan Attia; El-Kholy, Mohamed S.; Cerbo, Alessandro Di; Azzam, Mahmoud M.; Mahdy, E. A.; Khedr, Mariam H.E.; Elhady, Walaa M.

doi:10.1016/j.psj.2023.102487

Cited by 7 publications

(3 citation statements)

References 108 publications

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“…To detect the underlying mechanisms of ZnO-NPs cytotoxicity effect on the zebrafish olfactory organs, the mRNA expression of antioxidant, DNA damage, and apoptosis-related genes was evaluated by qRT-PCR [ 44 ] to support our TEM observations between the control and ZnO-NPs treated groups. It has been observed that ZnO-NPs alter mitochondrial structure and function, resulting in the generation of excessive reactive oxygen species (ROS) that weaken the antioxidant enzyme activity, altogether placing the olfactory cells at oxidative stress risk [ 46 , 47 , 48 , 49 ]. Accordingly, the mRNA expression of antioxidant-related genes, including sod1 , sod2 , gpx1a , gstp1.2 , and cat , was down-regulated in the treated olfactory rosette compared with the control one ( Figure 6 A).…”

Section: Resultsmentioning

confidence: 99%

Zinc Oxide Nanoparticles (ZnO-NPs) Induce Cytotoxicity in the Zebrafish Olfactory Organs via Activating Oxidative Stress and Apoptosis at the Ultrastructure and Genetic Levels

Al-Zahaby,

Farag,

Alagawany

et al. 2023

Animals

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Nanotechnology has gained tremendous attention because of its crucial characteristics and wide biomedical applications. Although zinc oxide nanoparticles (ZnO-NPs) are involved in many industrial applications, researchers pay more attention to their toxic effects on living organisms. Since the olfactory epithelium is exposed to the external environment, it is considered the first organ affected by ZnO-NPs. Herein, we demonstrated the cytotoxic effect of ZnO-NPs on the olfactory organ of adult zebrafish after 60 days post-treatment. We opted for this period when fishes stop eating their diet from the aquarium, appear feeble, and cannot swim freely. Our study demonstrated that ZnO-NPs induced significant malformations of the olfactory rosettes at histological, ultrastructural, and genetic levels. At the ultrastructure level, the olfactory lamellae appeared collapsed, malformed, and twisted with signs of degeneration and loss of intercellular connections. In addition, ZnO-NPs harmed sensory receptor and ciliated cells, microvilli, rodlet, crypt, and Kappe cells, with hyper-activity of mucous secretion from goblet cells. At the genetic level, ZnO-NPs could activate the reactive oxygen species (ROS) synthesis expected by the down-regulation of mRNA expression for the antioxidant-related genes and up-regulation of DNA damage, cell growth arrest, and apoptosis. Interestingly, ZnO-NPs affected the odor sensation at 60 days post-treatment (60-dpt) more than at 30-dpt, severely damaging the olfactory epithelium and irreparably affecting the cellular repairing mechanisms. This induced a dramatically adverse effect on the cellular endoplasmic reticulum (ER), revealed by higher CHOP protein expression, that suppresses the antioxidant effect of Nrf2 and is followed by the induction of apoptosis via the up-regulation of Bax expression and down-regulation of Bcl-2 protein.

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Section: Resultsmentioning

confidence: 99%

Zinc Oxide Nanoparticles (ZnO-NPs) Induce Cytotoxicity in the Zebrafish Olfactory Organs via Activating Oxidative Stress and Apoptosis at the Ultrastructure and Genetic Levels

Al-Zahaby,

Farag,

Alagawany

et al. 2023

Animals

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“…Similar to this, the study of Akosile et al (2023a) showed that the antioxidant status of broiler chicks was enhanced at hatch using 2 mg clove. In addition, Farag et al (2023) demonstrated that the in ovo feeding of polyphenolic compounds of chicoric and rosmarinic improved oxidative stress in chicks, while Abdelghani et al (2023) also demonstrated that 3 mg/egg of soy isoflavone had a positive effect on the antioxidant status of newly hatched chicks.…”

Section: Effect Of In Ovo Administration Of Phytog...mentioning

confidence: 99%

Potential Implication of in ovo Feeding of Phytogenics in Poultry Production

Akosile,

Kehinde,

Oni

et al. 2023

Translational Animal Science

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In industrialized nations, egg and poultry meat products are regarded as primary human protein requirements, and meeting this need necessitates a large number of day-old chicks. Hatchery's goals include maximizing revenue by achieving high hatchability with day-old birds of excellent quality. The advancement of technology has benefited the poultry sector since breeding and genetics technology have increased the rates of meat maturation in developing birds in a short period of time. Excessive use of in-feed antibiotics has been shown in studies to increase the chance of resistance to human infections. Bacterial resistance and antibiotic residues in animal products raised concerns about using antibiotics as growth promoters, eventually leading to a prohibition on using in-feed antibiotics in most industrialized nations. In ovo technology is a novel method for delivering bioactive chemicals to developing avian embryos. In ovo feeding technologies may provide additional nutrients to the embryos before hatching. The introduction of bioactive compounds has the potential to assist in decreasing and eventually eliminating the problems associated with traditional antibiotic delivery in chicken production. Phytobiotics were advocated as an alternative by researchers and dietitians. So far, several studies have been conducted on the use of phytogenic feed additives in poultry and swine feeding. They have primarily demonstrated that phytobiotics possess antibacterial, antioxidant, anti-inflammatory, and growth-stimulating properties. The antioxidant effect of phytobiotics can improve the stability of animal feed and increase the quality and storage duration of animal products. In general, the existing documentation indicates that phytobiotics improve poultry performance. In order to effectively and efficiently use the in ovo technique in poultry production and advance research in this area, it is important to have a thorough understanding of its potential as a means of nutrient delivery during the critical stage of incubation, its effects on hatching events and post-hatch performance, and the challenges associated with its use. Overall, this review suggests that in ovo feeding of phytobiotics have the potential to improve the antioxidant status and performance of chickens.

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“…Melissa officinalis (lemon balm) has a long history of being used in traditional medicine for the treatment of gastrointestinal ( GI ) tract disorders in human thanks to its spasmolytic ( Aubert et al, 2019 ; Sharifi-Rad et al, 2021 ) and stress reducing effects ( Nasri and Rafieian-Kopaei, 2013 ; Pineau et al, 2016 ; Frag et al, 2023 ). Additionally, in the combination with valerian, lemon balm is effective in the treatment of restlessness and dyssomnia in children ( Müller and Klement, 2006 ).…”

Section: Introductionmentioning

confidence: 99%

Modulation of chicken gut contractility by Melissa officinalis—ex vivo study

Posłuszny,

Chłopecka,

Suor-Cherer

et al. 2023

Poultry Science

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In ovo protective effects of chicoric and rosmarinic acids against Thiacloprid-induced cytotoxicity, oxidative stress, and growth retardation on newly hatched chicks

Cited by 7 publications

References 108 publications

Zinc Oxide Nanoparticles (ZnO-NPs) Induce Cytotoxicity in the Zebrafish Olfactory Organs via Activating Oxidative Stress and Apoptosis at the Ultrastructure and Genetic Levels

Zinc Oxide Nanoparticles (ZnO-NPs) Induce Cytotoxicity in the Zebrafish Olfactory Organs via Activating Oxidative Stress and Apoptosis at the Ultrastructure and Genetic Levels

Potential Implication of in ovo Feeding of Phytogenics in Poultry Production

Modulation of chicken gut contractility by Melissa officinalis—ex vivo study

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