In Ovo Injection of CHIR-99021 Promotes Feather Follicle Development via Modulating the Wnt Signaling Pathway and Transcriptome in Goose Embryos (Anser cygnoides)

Feng, Ziqiang; Gong, He; Fu, Jinhong; Xu, Xiaohui; Song, Yupu; Yan, Xiaomin; Mabrouk, Ichraf; Zhou, Yifang; Wang, Yudong; Fu, Xianou; Sui, Yuanyuan; Liu, Tuoya; Li, Chuanghang; Liu, Zebei; Xu, Tian; Sun, Li‐Zhong; Guo, Keying; Sun, Yanbo; Hu, Jingtao

doi:10.3389/fphys.2022.858274

Cited by 7 publications

(2 citation statements)

References 105 publications

(122 reference statements)

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“…The in ovo injection procedure was carried out as described previously [ 15 ]. Before injection, the border of the air sac or the yolk sac was identified under candlelight.…”

Section: Methodsmentioning

confidence: 99%

Notch mediates the glycolytic switch via PI3K/Akt signaling to support embryonic development

et al. 2023

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Background Energy metabolism disorder or insufficient energy supply during incubation will affect the development and survival of avian embryos. Especially, β-oxidation could not provide the continuous necessary energy for avian embryonic development due to the increasing energy demand under hypoxic conditions during the mid–late embryonic stages. The role and mechanism of hypoxic glycolysis replacing β-oxidation as the main source of energy supply for avian embryonic development in the mid–late stages is unclear. Results Here, we found that in ovo injection with glycolysis inhibitor or γ-secretase inhibitor both decreased the hepatic glycolysis level and impaired goose embryonic development. Intriguingly, the blockade of Notch signaling is also accompanied by the inhibition of PI3K/Akt signaling in the embryonic primary hepatocytes and embryonic liver. Notably, the decreased glycolysis and impaired embryonic growth induced by the blockade of Notch signaling were restored by activation of PI3K/Akt signaling. Conclusions Notch signaling regulates a key glycolytic switch in a PI3K/Akt-dependent manner to supply energy for avian embryonic growth. Our study is the first to demonstrate the role of Notch signaling-induced glycolytic switching in embryonic development, and presents new insight into the energy supply patterns in embryogenesis under hypoxic conditions. In addition, it may also provide a natural hypoxia model for developmental biology studies such as immunology, genetics, virology, cancer, etc. Graphical Abstract

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“…The in ovo injection procedure was carried out as described previously [ 15 ]. Before injection, the border of the air sac or the yolk sac was identified under candlelight.…”

Section: Methodsmentioning

confidence: 99%

Notch mediates the glycolytic switch via PI3K/Akt signaling to support embryonic development

et al. 2023

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“…The overexpression of Ctnnb1 can induce feather bud formation from the foot scale skin region of chicken embryos [ 15 ]. The in ovo injection of a Wnt activator significantly increases Ctnnb1/Lef1 gene expression levels and feather follicle development in ducks and geese [ 16 , 17 ]. Unlike SFRP1, which regulates Wnt/β-catenin activity during hair follicle development [ 18 , 19 ], DKK1 as a Wnt inhibitor exhibited strong signals during the development of feather primordia and buds in geese embryos [ 20 ].…”

Section: Feather Follicle Development In Chickensmentioning

confidence: 99%

Molecular Regulatory Mechanisms in Chicken Feather Follicle Morphogenesis

Ji,

Zhang,

et al. 2023

Genes

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In China, the sale of freshly slaughtered chickens is becoming increasingly popular in comparison with that of live chickens, and due to this emerging trend, the skin and feather follicle traits of yellow-feathered broilers have attracted a great deal of research attention. The feather follicle originates from the interaction between the epidermis and dermis in the early embryonic stage. Feather follicle morphogenesis is regulated by the Wnt, ectodysplasin (Eda), epidermal growth factor (EGF), fibroblast growth factor (FGF), bone morphogenetic protein (BMP), sonic hedgehog (Shh), Notch, and other signaling pathways that exist in epithelial and mesenchymal cells. The Wnt pathway is essential for feather follicle and feather morphogenesis. Eda interacts with Wnt to induce FGF expression, which attracts mesenchymal cell movement and aggregates to form feather follicle primordia. BMP acts as an inhibitor of the above signaling pathways to limit the size of the feather tract and distance between neighboring feather primordia in a dose-dependent manner. The Notch/Delta pathway can interact with the FGF pathway to promote feather bud formation. While not a part of the early morphogenesis of feather follicles, Shh and BMP signaling are involved in late feather branching. This review summarizes the roles of miRNAs/lncRNA in the regulation of feather follicle and feather growth and development and suggests topics that need to be solved in a future study. This review focuses on the regulatory mechanisms involved in feather follicle morphogenesis and analyzes the impact of SNP sites on feather follicle traits in poultry. This work may help us to understand the molecular regulatory networks influencing feather follicle growth and provide basic data for poultry carcass quality.

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In Ovo Injection of CHIR-99021 Promotes Feather Follicle Development via Modulating the Wnt Signaling Pathway and Transcriptome in Goose Embryos (Anser cygnoides)

Cited by 7 publications

References 105 publications

Notch mediates the glycolytic switch via PI3K/Akt signaling to support embryonic development

Notch mediates the glycolytic switch via PI3K/Akt signaling to support embryonic development

Molecular Regulatory Mechanisms in Chicken Feather Follicle Morphogenesis

Dermal FOXO3 activity in response to Wnt/β-catenin signaling is required for feather follicle development of goose embryos (Anser cygnoides)

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