In‐ovo imaging using ostrich eggs—Evaluation of physiological embryonal development on computed tomography

Winkens, Thomas; Christl, Anna; Kühnel, Christian; Ndum, Ferdinand; Seifert, Philipp; Greiser, Julia; Freesmeyer, Martin

doi:10.1111/azo.12400

Cited by 7 publications

(12 citation statements)

References 28 publications

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“…This study aims at (1) exploring the feasibility of biomagnetism to detect embryonal motion and cardiac signals and using these findings (2) to investigate the effect of isoflurane anesthesia on ostrich embryos. The conclusions drawn from this study are needed to plan and execute further experiments regarding in-ovo imaging using ostrich eggs, that is, in terms of establishing a protocol for successful immobilization.…”

Section: Experimental Biology Andmentioning

confidence: 99%

“…1 Preliminary studies report on the feasibility and technical success of positron emission tomography/ computed tomography (PET/CT) and computed tomography (CT) examinations; however, embryonal motion was identified as a major limitation regarding imaging. 1,2 The advantages of using ostrich eggs for preclinical imaging instead of common animal testing consist of the following: (1) Regular scanners commonly used in clinical routine in humans are sufficient to provide good image quality. Due to the large size of approximately 20 × 15 cm, there is no need for dedicated small animal imaging devices, thus bypassing costly investments.…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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In-ovo imaging using ostrich eggs: Biomagnetism for detection of cardiac signals and embryonal motion

Freesmeyer

Hermeyer

Kühnel

et al. 2022

Exp Biol Med (Maywood)

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In-ovo imaging using ostrich eggs has been described as a potential alternative to common animal testing. The main advantage is its independence from small animal imaging devices as ostrich eggs provide good image quality on regular CT, MRI, or PET used in examinations of humans. However, embryonal motion during dynamic imaging studies produce artifacts. The aims of this study were (1) to explore the feasibility of biomagnetism to detect cardiac signals and embryonal motion and to use these findings (2) to investigate the effect of isoflurane anesthesia on ostrich embryos. A standard magnetoencephalography developed for brain studies was used to detect embryonal signals of ostrich eggs on developmental day 34. Signals were instantly shown on a screen and data were also postprocessed. For assessing the effects of anesthesia, nine ostrich eggs were investigated using isoflurane 6% for 90 min. Biomagnetic signals were recorded simultaneously. A control group consisting of eight different ostrich eggs was also investigated. Cardiac signals similar to electrocardiography were observed in all eggs. Postprocessing revealed frequent motion of embryos without anesthesia. The exposure to isoflurane led to a significant decrease in motion signals in 9/9 ostrich embryos after 8 min. Motion was significantly reduced in the isoflurane group versus control group. There were no isoflurane-related deaths. This study shows that biomagnetism is feasible to detect cardiac signals and motion of ostrich embryos in-ovo. Application of isoflurane is safe and leads to a rapid decrease in embryonal motion, which is an important prerequisite for the implementation of in-ovo imaging using ostrich eggs.

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Section: Experimental Biology Andmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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In-ovo imaging using ostrich eggs: Biomagnetism for detection of cardiac signals and embryonal motion

Freesmeyer

Hermeyer

Kühnel

et al. 2022

Exp Biol Med (Maywood)

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“…A similar approach was employed to estimate ages in a rare series of kiwi embryos (Prier et al, 2013). Future studies may need to rely on such creative approaches, as well as emerging technologies that may permit the efficient and noninvasive ( in ovo ) characterization of embryonic features in natural and laboratory settings (Freesmeyer et al, 2018; Henning et al, 2011; Winkens et al, 2021). We recommend the employment of modern visualization and quantitative tools coupled with the targeted sampling of late developmental stages that display lineage‐specific morphological traits.…”

Section: Domestic Species and The Future Of Comparative Avian Embryologymentioning

confidence: 99%

Domestication and the comparative embryology of birds

Cordero

Werneburg

2022

J Exp Zool Pt B

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Studies of domesticated animals have greatly contributed to our understanding of avian embryology. Foundational questions in developmental biology were motivated by Aristotle's observations of chicken embryos. By the 19th century, the chicken embryo was at the center stage of developmental biology, but how closely does this model species mirror the ample taxonomic diversity that characterizes the avian tree of life? Here, we provide a brief overview of the taxonomic breadth of comparative embryological studies in birds. We particularly focused on staging tables and papers that attempted to document the timing of developmental transformations. We show that most of the current knowledge of avian embryology is based on Galliformes (chicken and quail) and Anseriformes (duck and goose). Nonetheless, data are available for some ecologically diverse avian subclades, including Struthioniformes (e.g., ostrich, emu) and Sphenisciformes (penguins). Thus far, there has only been a handful of descriptive embryological studies in the most speciose subclade of Aves, that is, the songbirds (Passeriniformes). Furthermore, we found that temporal variances for developmental events are generally uniform across a consensus chronological sequence for birds. Based on the available data, developmental trajectories for chicken and other model species appear to be highly similar. We discuss future avenues of research in comparative avian embryology in light of the currently available wealth of data on domesticated species and beyond.

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“…In ovo imaging using ostrich eggs has been already described as a good alternative concept not only to common animal testing using rats or mice but also to chicken eggs. Due to their large size of approximately 20 × 15 cm, there is no need for dedicated small animal imaging devices, thus bypassing costly investments [ 23 , 24 , 25 ].…”

Section: Introductionmentioning

confidence: 99%

A Novel Breast Cancer Xenograft Model Using the Ostrich Chorioallantoic Membrane—A Proof of Concept

Pomraenke,

Bolney,

Winkens

et al. 2023

Veterinary Sciences

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The avian chorioallantoic membrane (CAM) assay has attracted scientific attention in cancer research as an alternative or complementary method for in vivo animal models. Here, we present a xenograft model based on the ostrich (struthio camelus) CAM assay for the first time. The engraftment of 2 × 106 breast cancer carcinoma MDA-MB-231 cells successfully lead to tumor formation. Tumor growth monitoring was evaluated in eight fertilized eggs after xenotransplantation. Cancer cells were injected directly onto the CAM surface, close to a well-vascularized area. Histological analysis confirmed the epithelial origin of tumors. The CAM of ostrich embryos provides a large experimental surface for the xenograft, while the comparably long developmental period allows for a long experimental window for tumor growth and treatment. These advantages could make the ostrich CAM assay an attractive alternative to the well-established chick embryo model. Additionally, the large size of ostrich embryos compared to mice and rats could help overcome the limitations of small animal models. The suggested ostrich model is promising for future applications, for example, in radiopharmaceutical research, the size of the embryonal organs may compensate for the loss in image resolution caused by physical limitations in small animal positron emission tomography (PET) imaging.

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In‐ovo imaging using ostrich eggs—Evaluation of physiological embryonal development on computed tomography

Abstract: This is an open access article under the terms of the Creat ive Commo ns Attri bution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

Cited by 7 publications

References 28 publications

In-ovo imaging using ostrich eggs: Biomagnetism for detection of cardiac signals and embryonal motion

In-ovo imaging using ostrich eggs: Biomagnetism for detection of cardiac signals and embryonal motion

Domestication and the comparative embryology of birds

A Novel Breast Cancer Xenograft Model Using the Ostrich Chorioallantoic Membrane—A Proof of Concept

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