In ovo administration of a phage cocktail partially prevents colibacillosis in chicks

Nicolas, Marianne; Faurie, Arnaud; Girault, Mylène; Lavillatte, Sébastien; Menanteau, Pierrette; Chaumeil, Thierry; Riou, Mickaël; Velge, Philippe; Schouler, Catherine

doi:10.1016/j.psj.2023.102967

Cited by 5 publications

(5 citation statements)

References 41 publications

(49 reference statements)

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“…Two of the four phages were found in the caecum of the chicks on day 7 post-hatch, and the chicks treated with the bacteriophages did not develop colibacillosis lesions. The pathogenic bacteria retrieved from the intestines were significantly lower than those from the controls [104]. These investigations proved that the in ovo inoculation of phages (better in amniotic fluid) can cause protection against colibacillosis despite not achieving clearance of the target bacteria.…”

Section: Poultrymentioning

confidence: 69%

Bacteriophage Therapy in Companion and Farm Animals

Bianchessi,

De Bernardi,

Vigorelli

et al. 2024

Antibiotics

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Bacteriophages, which are viruses with restricted tropism for bacteria, have been employed for over a century as antimicrobial agents; they have been largely abandoned in Western countries but are constantly used in Eastern European countries with the advent of antibiotics. In recent decades, the growing spread of multidrug-resistant bacteria, which pose a serious threat to worldwide public health, imposed an urgent demand for alternative therapeutic approaches to antibiotics in animal and human fields. Based on this requirement, numerous studies have been published on developing and testing bacteriophage-based therapy. Overall, the literature largely supports the potential of this perspective but also highlights the need for additional research as the current standards are inadequate to receive approval from regulatory authorities. This review aims to update and critically revise the current knowledge on the application of bacteriophages to treat bacterial-derived infectious diseases in animals in order to provide topical perspectives and innovative advances.

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Section: Poultrymentioning

confidence: 69%

Bacteriophage Therapy in Companion and Farm Animals

Bianchessi,

De Bernardi,

Vigorelli

et al. 2024

Antibiotics

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“…Today, its commercial application around the world continues to prioritize vaccination as its main objective. Based on research already carried out [ 46 , 47 , 48 , 49 , 50 , 51 ], in ovo injections offer a range of advantages in poultry farming, such as: The in ovo injection technique does not require very complex professional training to be administered. It can be considered the best option for early and systemic immunization of poultry, with the absence of pain and stress.…”

Section: What Is the In Ovo Injection Technique?mentioning

confidence: 99%

“…Today, its commercial application around the world continues to prioritize vaccination as its main objective. Based on research already carried out [46][47][48][49][50][51], in ovo injections offer a range of advantages in poultry farming, such as:…”

Section: What Is the In Ovo Injection Technique?mentioning

confidence: 99%

Control of Escherichia coli in Poultry Using the In Ovo Injection Technique

Oliveira,

McManus,

dos Santos

2024

Antibiotics

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Pathogens, such as Escherichia coli (E. coli), have been identified as significant causes of poultry mortality. Poultry can serve as potential sources of E. coli transmission, even when asymptomatic, posing a substantial threat to food safety and human health. The in ovo administration of antimicrobials is crucial for preventing and/or effectively combating acute and chronic infections caused by poultry pathogens. To achieve this goal, it is critical that antimicrobials are properly injected into embryonic fluids, such as the amnion, to reach target tissues and trigger robust antimicrobial responses. Several protocols based on antimicrobials were evaluated to meet these requirements. This review analyzed the impacts of antimicrobial substances injected in ovo on the control of E. coli in poultry. The reduction in infection rates, resulting from the implementation of in ovo antimicrobials, combined with efforts aimed at hygienic-sanitary action plans in poultry sheds, reinforces confidence that E. coli can be contained before causing large scale damage. For example, antimicrobial peptides and probiotics have shown potential to provide protection to poultry against infections caused by E. coli. Issues related to the toxicity and bacterial resistance of many synthetic chemical compounds represent challenges that need to be overcome before the commercial application of in ovo injection protocols focused on microbiological control.

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“…Avian colibacillosis (AC) is an infectious disease caused by the avian pathogen E. coli (APEC), that results in fibrinoid lesions around internal organs ( Gao et al., 2018 ; Nicolas et al., 2023 ). This disease can lead to primary infection as well as secondary infection with other viruses or pathogens ( Sargeant et al., 2019 ; Walker et al., 2020 ).…”

Section: Introductionmentioning

confidence: 99%

CATH-2-derived antimicrobial peptide inhibits multidrug-resistant Escherichia coli infection in chickens

Hao,

Shi,

Chen

et al. 2024

Front. Cell. Infect. Microbiol.

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Avian colibacillosis (AC), caused by infection with Escherichia coli (E. coli), is a major threat to poultry health, food safety and public health, and results in high mortality and significant economic losses. Currently, new drugs are urgently needed to replace antibiotics due to the continuous emergence and increasing resistance of multidrug-resistant (MDR) strains of E. coli caused by the irrational use of antibiotics in agriculture and animal husbandry. In recent years, antimicrobial peptides (AMPs), which uniquely evolved to protect the host, have emerged as a leading alternative to antibiotics in clinical settings. CATH-2, a member of the antimicrobial cathelicidin peptide family, has been reported to have antibacterial activity. To enhance the antimicrobial potency and reduce the adverse effects on animals, we designed five novel AMPs, named C2-1, C2-2, C2-3, C2-4 and C2-5, based on chicken CATH-2, the secondary structures of these AMPs were consistently α-helical and had an altered net charge and hydrophobicity compared to those of the CATH-2 (1-15) sequences. Subsequently, the antimicrobial activities of CATH-2 (1-15) and five designed peptides against MDR E. coli were evaluated in vitro. Specifically, C2-2 showed excellent antimicrobial activity against either the ATCC standard strain or veterinary clinical isolates of MDR E. coli, with concentrations ranging from 2-8 μg/mL. Furthermore, C2-2 maintained its strong antibacterial efficacy under high temperature and saline conditions, demonstrating significant stability. Similarly, C2-2 retained a high level of safety with no significant hemolytic activity on chicken mature red blood cells or cytotoxicity on chicken kidney cells over the concentration range of 0-64 μg/mL. Moreover, the administration of C2-2 improved the survival rate and reduced the bacterial load in the heart, liver and spleen during MDR E. coli infection in chickens. Additionally, pathological damage to the heart, liver and intestine was prevented when MDR E. coli infected chickens were treated with C2-2. Together, our study showed that C2-2 may be a promising novel therapeutic agent for the treatment of MDR E. coli infections and AC.

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In ovo administration of a phage cocktail partially prevents colibacillosis in chicks

Cited by 5 publications

References 41 publications

Bacteriophage Therapy in Companion and Farm Animals

Bacteriophage Therapy in Companion and Farm Animals

Control of Escherichia coli in Poultry Using the In Ovo Injection Technique

CATH-2-derived antimicrobial peptide inhibits multidrug-resistant Escherichia coli infection in chickens

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