In interleukin‐7‐transgenic mice, increasing B lymphopoiesis increases follicular but not marginal zone B cell numbers

Ceredig, Rhodri; Bosco, Nabil; Maye, Patrice N; Andersson, Jan; Rolink, Antonius

doi:10.1002/eji.200324134

Cited by 20 publications

(15 citation statements)

References 45 publications

(65 reference statements)

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“…This is consistent with our previous data using IL7Tg.B6 mice, where the increase in mature B cell compartments is simply a consequence of increasing BM B lymphopoiesis and not due to a role for IL-7 in mature B cell survival (32). Thus, as previously reported, T and B cell homeostasis are independent of one another and depend on different resources (55).…”

Section: Discussionsupporting

confidence: 53%

“…injection and 1 mg/ml BrdU in their drinking water for 5 days. Recipient mice were sacrificed at day 28 after T cell transfer, and spleen cell suspensions were prepared, surface stained as described above, washed, fixed, and permeabilized before labeling with anti-BrdU mAb as described previously (32,33) using the BrdU flow kit (BD Pharmingen). Then, 10 6 cells were analyzed by FACS.…”

Section: In Vivo Brdu Labelingmentioning

confidence: 99%

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Effects of Increasing IL-7 Availability on Lymphocytes during and after Lymphopenia-Induced Proliferation

Bosco

Agenès

Ceredig

2005

The Journal of Immunology

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IL-7 is critically involved in regulating peripheral T cell homeostasis. To investigate the role of IL-7 on lymphopenia-induced proliferation of polyclonal lymphocytes, we have transferred CFSE-labeled cells into a novel T-lymphopenic, IL-7-transgenic mouse line. Results obtained indicate that T and B cells do not respond in the same way to IL-7-homeostatic signals. Overexpression of IL-7 enhances proliferation of both CD4+ and CD8+ T cells but with distinctly temporal effects. Expansion of naturally arising CD4+-regulatory T cells was like that of conventional CD4+ T cells. IL-7 had no effect on B cell proliferation. By immunohistology, transferred T cells homed to T cell areas of spleen lymphoid follicles. Increasing IL-7 availability enhanced T cell recovery by promoting cell proliferation and reducing apoptosis during early stages of lymphopenia-induced proliferation. Taken together, these results provide new insights into the pleiotropic effects of IL-7 on lymphopenia-induced T cell proliferation.

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Section: Discussionsupporting

confidence: 53%

Section: In Vivo Brdu Labelingmentioning

confidence: 99%

Effects of Increasing IL-7 Availability on Lymphocytes during and after Lymphopenia-Induced Proliferation

Bosco

Agenès

Ceredig

2005

The Journal of Immunology

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“…These results are consistent with the phenotype in Il7ra 2/2 mice, in which the peripheral B cell defects are most apparent in the FO B cell compartment (46). Moreover, it was shown that the FO B cells increase in number, whereas the proportion of MZ B cells decreases in IL-7 transgenic mice (47). The second defect in Srg3-deficient pro-B cells is the impaired expression of B lineage-specific genes, such as E47 (Tcfe2a), Ebf1, and Pax5.…”

Section: Discussionsupporting

confidence: 88%

The SWI/SNF-like BAF Complex Is Essential for Early B Cell Development

Choi

Jeon

et al. 2012

The Journal of Immunology

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During the process of B cell development, transcription factors, such as E2A and Ebf1, have been known to play key roles. Although transcription factors and chromatin regulators work in concert to direct the expression of B lineage-specific genes, little is known about the involvement of regulators for chromatin structure during B lymphopoiesis. In this article, we show that deletion of Srg3/mBaf155, a scaffold subunit of the SWI/SNF-like BAF complex, in the hematopoietic lineage caused defects at both the common lymphoid progenitor stage and the transition from pre–pro-B to early pro-B cells due to failures in the expression of B lineage-specific genes, such as Ebf1 and Il7ra, and their downstream target genes. Moreover, mice that were deficient in the expression of Brg1, a subunit of the complex with ATPase activity, also showed defects in early B cell development. We also found that the expression of Ebf1 and Il7ra is directly regulated by the SWI/SNF-like BAF complex. Thus, our results suggest that the SWI/SNF-like BAF complex facilitates early B cell development by regulating the expression of B lineage-specific genes.

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“…MZ and B-1 B cell genesis may thus be more dependent on TSLP, while IL-7 may be more important for the generation of FO B cells. In support of this, FO but not MZ and B-1 B cell numbers are increased in transgenic mice overexpressing IL-7 [38].…”

Section: Discussionmentioning

confidence: 67%

Impaired B‐1 and B‐2 B cell development and atypical splenic B cell structures in IL‐7 receptor‐deficient mice

et al. 2004

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The cytokine IL-7 and its receptor are essential for normal B and T lymphopoiesis. We have analyzed the role of this receptor in B cell development throughout ontogeny in IL-7 receptor a-deficient mice. We demonstrate that the IL-7 receptor becomes progressively more important with age. B lymphopoiesis takes place, albeit at reduced levels, in fetal liver and bone marrow of young mice, but is arrested in adults. The outcome is a severe reduction, from an early age, in peripheral B cells including follicular, marginal zone and B-1 B cells as well as perturbed splenic B cell structures, which are restored after adoptive transfer of normal spleen cells. We conclude that in the absence of the IL-7 receptor, the residual B lymphopoiesis occurring early in ontogeny must be facilitated by another component, whereas the IL-7 receptor is the key factor in adults. The impairment of marginal zone and B-1 B cells in IL-7 receptor-but not IL-7-deficient mice suggests non-redundant functions for the IL-7 receptor ligands, IL-7 and thymic stromal lymphopoietin.

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In interleukin‐7‐transgenic mice, increasing B lymphopoiesis increases follicular but not marginal zone B cell numbers

Cited by 20 publications

References 45 publications

Effects of Increasing IL-7 Availability on Lymphocytes during and after Lymphopenia-Induced Proliferation

Effects of Increasing IL-7 Availability on Lymphocytes during and after Lymphopenia-Induced Proliferation

The SWI/SNF-like BAF Complex Is Essential for Early B Cell Development

Impaired B‐1 and B‐2 B cell development and atypical splenic B cell structures in IL‐7 receptor‐deficient mice

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