In <i>vitro</i> inhibition of <i>Helicobacter pylori</i> by extracts of thyme

Tabak, Mina; Armon, Robert; Potasman, Israel; Néeman, I.

doi:10.1111/j.1365-2672.1996.tb03272.x

Cited by 68 publications

(37 citation statements)

References 22 publications

(8 reference statements)

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“…However, carvacrol constitutes less than 50% of the oil, indicating that other compounds may interact with carvacrol to confer the high level of activity to the oil. Cinnamon oil was used as a positive control, as it has already been demonstrated that the oil and its extracts are active against H. pylori (18,19).…”

Section: Disk Inhibition Assay Of H Pylori Growthmentioning

confidence: 99%

Essential Oils as Components of a Diet-Based Approach to Management of Helicobacter Infection

Bergonzelli

Donnicola

Porta

et al. 2003

Antimicrob Agents Chemother

101

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An increased density of Helicobacter pylori in the gastric mucosa can be associated with more severe gastritis and an increased incidence of peptic ulcers. Therefore, people with asymptomatic gastritis would certainly benefit from a nutritional approach to help them manage the infection and therefore decrease the risk of development of associated pathologies. We analyzed the activities of 60 essential oils against H. pylori P1 and identified 30 oils that affected growth, with in vitro inhibition zones ranging between 0.7 and 6.3 cm in diameter. We further analyzed the effects of 16 oils with different activities on H. pylori P1 viability. Fifteen showed strong bactericidal activities, with minimal bactericidal concentrations after 24 h ranging from 0.02 to 0.1 g/liter at pH 7.4. Even though slight variations in activities were observed, the essential oils that displayed the strongest bactericidal potentials against H. pylori P1 were also active against other Helicobacter strains tested. Among the pure constituents of different essential oils tested, carvacrol, isoeugenol, nerol, citral, and sabinene exhibited the strongest anti-H. pylori activities. Although oral treatment of H. pylori SS1-infected mice with carrot seed oil did not result in significant decreases in the bacterial loads in the treated animals compared to those in the control animals, in all experiments performed, the infection was cleared in 20 to 30% of carrot seed oil-treated animals. Our results indicate that essential oils are unlikely to be efficient anti-Helicobacter agents in vivo. However, their effects may not be irrelevant if one plans to use them as food additives to complement present therapies.

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Section: Disk Inhibition Assay Of H Pylori Growthmentioning

confidence: 99%

Essential Oils as Components of a Diet-Based Approach to Management of Helicobacter Infection

Bergonzelli

Donnicola

Porta

et al. 2003

Antimicrob Agents Chemother

101

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“…The occurrence of strains resistant to antibiotics would be expected to increase, and it is nowadays important to search for nonantibiotic substances with anti-H. pylori activity. Herbal extracts and essential oils have been used as traditional medicines for thousands of years all over the world, and their anti-H. pylori activity has been widely demonstrated in vitro (2)(3)(4)(5). Many foodstuffs have also exhibited inhibitory activity against the growth of H. pylori in vitro, among others, red wine (6,7), sprouted peas (8), green tea (9), and cranberry juice (10).…”

Section: Introductionmentioning

confidence: 99%

In Vitro Activity of Olive Oil Polyphenols against Helicobacter pylori

Romero

Medina

Vargas

et al. 2007

J. Agric. Food Chem.

168

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Helicobacter pylori is linked to a majority of peptic ulcers and to some types of gastric cancer, and resistance of the microorganism to antibiotic treatment is now found worldwide. Virgin olive oil is an unrefined vegetable oil that contains a significant amount of phenolic compounds. Under simulated conditions, we have demonstrated that these substances can diffuse from the oil into the gastric juice and be stable for hours in this acidic environment. In vitro, they exerted a strong bactericidal activity against eight strains of H. pylori, three of them resistant to some antibiotics. Among the phenolic compounds, the dialdehydic form of decarboxymethyl ligstroside aglycon showed the strongest bactericidal effect at a concentration as low as 1.3 μg/mL. Although the experimental conditions are different from other reported works, this bactericidal concentration is much lower than those found for phenolic compounds from tea, wine, and plant extracts. These results open the possibility of considering virgin olive oil a chemopreventive agent for peptic ulcer or gastric cancer, but this bioactivity should be confirmed in vivo in the future. Keywords: Olive oil; phenolic compounds; simulated digestion, Helicobacter pylori; antimicrobial

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“…A wide spectrum of pharmacological activities have been described for thyme, such as antioxidant (13), antibacterial (26,27) and antifungal (28,29) properties. These activities have been attributed to some widely distributed species of thyme.…”

Section: Discussionmentioning

confidence: 99%

Cytotoxic effect of essential oil of thyme (Thymus broussonettii) on the IGR-OV1 tumor cells resistant to chemotherapy

Mbarek

Mouse

Jaafari

et al. 2007

Braz J Med Biol Res

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The anti-tumor effect of the Moroccan endemic thyme (Thymus broussonettii) essential oil (EOT) was investigated in vitro using the human ovarian adenocarcinoma IGR-OV1 parental cell line OV1/P and its chemoresistant counterparts OV1/adriamycin (OV1/ADR), OV1/vincristine (OV1/VCR), and OV1/cisplatin (OV1/CDDP). All of these cell lines elicited various degrees of sensitivity to the cytotoxic effect of EOT. The IC 50 values (mean ± SEM, v/v) were 0.40 ± 0.02, 0.39 ± 0.02, 0.94 ± 0.05, and 0.65 ± 0.03% for OV1/P, OV1/ ADR, OV1/VCR, and OV1/CDDP, respectively. Using the DBA-2/ P815 (H 2 d ) mouse model, tumors were developed by subcutaneous grafting of tumor fragments of similar size obtained from P815 (murin mastocytoma cell line) injected in donor mouse. Interestingly, intratumoral injection of EOT significantly reduced solid tumor development. Indeed, by the 30th day of repeated EOT treatment, the tumor volumes of the animals were 2.00 ± 0.27, 1.35 ± 0.20, and 0.85 ± 0.18 cm 3 after injection with 10, 30, or 50 µL per 72 h (six times), respectively, as opposed to 3.88 ± 0.50 cm 3 for the control animals. This tumoricidal effect was associated with a marked decrease of mouse mortality. In fact, in these groups of mice, the recorded mortality by the 30th day of treatment was 30 ± 4, 18 ± 4, and 8 ± 3%, respectively, while the control animals showed 75 ± 10% of mortality. These data indicate that the EOT which contains carvacrol as the major component has an important in vitro cytotoxic activity against tumor cells resistant to chemotherapy as well as a significant antitumor effect in mice. However, our data do not distinguish between carvacrol and the other components of EOT as the active factor.

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In vitro inhibition of Helicobacter pylori by extracts of thyme

Cited by 68 publications

References 22 publications

Essential Oils as Components of a Diet-Based Approach to Management of Helicobacter Infection

Essential Oils as Components of a Diet-Based Approach to Management of Helicobacter Infection

In Vitro Activity of Olive Oil Polyphenols against Helicobacter pylori

Cytotoxic effect of essential oil of thyme (Thymus broussonettii) on the IGR-OV1 tumor cells resistant to chemotherapy

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