In vitro Binding of Pasteurella multocida Cell Wall Preparations to Tracheal Mucus of Cattle and Swine and to a Tracheal Epithel Cell Wall Preparation of Cattle

Abstract

Outer membrane preparations of various Pasteurella isolates (Pasteurella multocida and some other Pasteurella species) from cattle and swine were extracted by N-lauryl-sarcosine sodium salt. Capsular extracts were prepared by heat treatment. Both preparations bound to epithel cell wall preparations (ECW) of trachea from cattle and to tracheal mucus of cattle and swine. Binding was demonstrated by an enzyme-linked immunosorbent assay (ELISA). Distinct high adherence values were shown by the greater part of membrane preparations of mucoid Pasteurella strains, especially when originating from cattle. U.S.Copyright Clearance CenterCode Statement: 0931 -1793/91/3810-0721$02.50/0 P. rn. nz.

Virulence of capsulated and noncapsulated isolates of Pasteurella multocida and their adherence to porcine respiratory tract cells and mucus

“…Although adhesion seems to be an important step in the pathogenesis of atrophic rhinitis (28) and several studies have evaluated the attachment of P. multocida to various porcine respiratory tract cells and to porcine respiratory tract mucus (4,10,14,21,24,27,28,41), the adhesins of P. multocida have not been identified. Thus, the purpose of the present study was to examine the role of P. multocida MATERIALS AND METHODS Bacterial isolates and growth conditions.…”

“…Piglets were challenged four times intranasally (0.5 ml per nostril) with a suspension containing 106 CFU/ml (first inoculation) or 109 CFU/ml (three other inoculations) 4 days in a row. Eight uninoculated piglets, maintained in a separate unit, were used as controls.…”