In
            <i>Pseudomonas syringae</i>
            pv. phaseolicola, Expression of the
            <i>argK</i>
            Gene, Encoding the Phaseolotoxin-Resistant Ornithine Carbamoyltransferase, Is Regulated Indirectly by Temperature and Directly by a Precursor Resembling Carbamoylphosphate

López‐López, Karina; Hernández-Flores, José Luis; Cruz‐Aguilar, Marisa; Alvarez‐Morales, Ariel

doi:10.1128/jb.186.1.146-153.2004

Cited by 22 publications

(33 citation statements)

References 32 publications

(48 reference statements)

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“…The latter (inorganic) moiety is proposed to inactivate another repressor, R 2 , thereby derepressing argK. Thus, although temperature control of argK is indirect, the onset of resistance and toxin production are nevertheless coupled [126,194].…”

Section: Target Ornithine Carbamoyltransferase: Phaseolotoxinmentioning

confidence: 98%

“…Two genes encoding OCT activity, argF and argK, are present in the producer [170] and one of them (argK, encoding ROCT) is located within the phaseolotoxin-biosynthetic cluster [1]. According to one model [126], temperature downshift is sensed in P. syringae pv. phaseolicola by an unknown mechanism that inactivates a transcriptional repressor, R 1 .…”

Section: Target Ornithine Carbamoyltransferase: Phaseolotoxinmentioning

confidence: 99%

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Avoidance of suicide in antibiotic-producing microbes

Cundliffe

Demain

2010

J Ind Microbiol Biotechnol

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Many microbes synthesize potentially autotoxic antibiotics, mainly as secondary metabolites, against which they need to protect themselves. This is done in various ways, ranging from target-based strategies (i.e. modification of normal drug receptors or de novo synthesis of the latter in drug-resistant form) to the adoption of metabolic shielding and/or efflux strategies that prevent drug-target interactions. These self-defence mechanisms have been studied most intensively in antibiotic-producing prokaryotes, of which the most prolific are the actinomycetes. Only a few documented examples pertain to lower eukaryotes while higher organisms have hardly been addressed in this context. Thus, many plant alkaloids, variously described as herbivore repellents or nitrogen excretion devices, are truly antibiotics-even if toxic to humans. As just one example, bulbs of Narcissus spp. (including the King Alfred daffodil) accumulate narciclasine that binds to the larger subunit of the eukaryotic ribosome and inhibits peptide bond formation. However, ribosomes in the Amaryllidaceae have not been tested for possible resistance to narciclasine and other alkaloids. Clearly, the prevalence of suicide avoidance is likely to extend well beyond the remit of the present article.

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Section: Target Ornithine Carbamoyltransferase: Phaseolotoxinmentioning

confidence: 98%

Section: Target Ornithine Carbamoyltransferase: Phaseolotoxinmentioning

confidence: 99%

Avoidance of suicide in antibiotic-producing microbes

Cundliffe

Demain

2010

J Ind Microbiol Biotechnol

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“…However, only weak binding of the putative repressor protein to the TRR sites in argK has been postulated (23). Furthermore, the TRR domains in argK may not be involved in its regulation since we have shown that this gene is not directly regulated by temperature but most likely is regulated through induction mediated by a precursor of phaseolotoxin resembling carbamoylphosphate (11). Therefore, there seem to be two proteins that bind to the TRR, one protein which binds tightly to strictly temperature-regulated promoters and a different protein which acts as a repressor for argK with only weak binding to the TRR and which is not temperature regulated.…”

mentioning

confidence: 94%

“…It has been shown previously that in fact argK is not directly regulated by temperature but most likely is regulated by a precursor of phaseolotoxin resembling carbamoylphosphate (11). In Pseudomonas aeruginosa argF is repressed when growth occurs in media that provide high levels of exogenous arginine, such as King's medium B (KB), together with the repressor protein ArgR, which also represses the carAB operon involved in carbamoylphosphate biosynthesis (20,21).…”

mentioning

confidence: 99%

The Global Arginine Regulator ArgR Controls Expression of argF in Pseudomonas syringae pv. phaseolicola but Is Not Required for the Synthesis of Phaseolotoxin or for the Regulated Expression of argK

et al. 2004

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“…Likewise, phaseolotoxin production is thermoregulated in P. syringae pv. phaseolicola by a process involving a small, noncoding RNA and, potentially, a metabolic intermediate, although the details of this process are currently unknown (21)(22)(23). More generally, it is unknown whether there are any conserved global thermoregulators in P. syringae similar to those described in E. coli.…”

mentioning

confidence: 99%

Involvement of rppH in Thermoregulation in Pseudomonas syringae

2014

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Temperature, among other environmental factors, influences the incidence and severity of many plant diseases. Likewise, numerous traits, including the expression of virulence factors, are regulated by temperature. Little is known about the underlying genetic determinants of thermoregulation in plant-pathogenic bacteria. Previously, we showed that the expression of both fliC (encoding flagellin) and syfA (encoding a nonribosomal polypeptide synthetase) was suppressed at high temperatures in Pseudomonas syringae. In this work, we used a high-throughput screen to identify mutations that conferred overexpression of syfA at elevated temperatures (28°C compared to 20°C). Two genes, Psyr_2474, encoding an acyl-coenzyme A (CoA) dehydrogenase, and Psyr_4843, encoding an ortholog of RppH, which in Escherichia coli mediates RNA turnover, contribute to thermoregulation of syfA. To assess the global role of rppH in thermoregulation in P. syringae, RNA sequencing was used to compare the transcriptomes of an rppH deletion mutant and the wild-type strain incubated at 20°C and 30°C. The disruption of rppH had a large effect on the temperature-dependent transcriptome of P. syringae, affecting the expression of 569 genes at either 20°C or 30°C but not at both temperatures. Intriguingly, RppH is involved in the thermoregulation of ribosome-associated proteins, as well as of RNase E, suggesting a prominent role of rppH on the proteome in addition to its effect on the transcriptome. Temperature is an important environmental factor that influences many aspects of microbial physiology and profoundly affects an organism's ability to survive and reproduce (1). Since microorganisms must appropriately both perceive and respond to changes in temperature, they possess some form of thermoregulated gene expression. Certain temperature responses appear to be conserved across diverse bacterial species, such as the cold shock and heat shock responses (2, 3). The stimulatory signals and regulatory mechanisms of these shock responses are also largely conserved. However, in addition to the shock responses, which maintain cellular functions after large and rapid temperature shifts, most bacterial species also possess more specialized forms of thermoregulation whose role is not necessarily to return the cell to homeostasis but, rather, to reprogram cells for fitness in the altered environment. For example, animal pathogens often use host body temperature as a cue to express virulence factors (4-6). In this setting, members of many taxa suppress the production of flagellar genes since they encode immune-eliciting antigens (i.e., flagellin) (7). While the suppression of flagellin at host temperatures is common in such animal pathogens, the stages within the flagellar hierarchy and the mechanisms by which such regulation is achieved differ between organisms (cf. E. coli [8], Yersinia [9-11], and Listeria [12]). Common thermoregulated traits, such as motility, therefore do not necessarily have conserved mechanisms of regulation. This may be a consequence ...

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In Pseudomonas syringae pv. phaseolicola, Expression of the argK Gene, Encoding the Phaseolotoxin-Resistant Ornithine Carbamoyltransferase, Is Regulated Indirectly by Temperature and Directly by a Precursor Resembling Carbamoylphosphate

Cited by 22 publications

References 32 publications

Avoidance of suicide in antibiotic-producing microbes

Avoidance of suicide in antibiotic-producing microbes

The Global Arginine Regulator ArgR Controls Expression of argF in Pseudomonas syringae pv. phaseolicola but Is Not Required for the Synthesis of Phaseolotoxin or for the Regulated Expression of argK

Involvement of rppH in Thermoregulation in Pseudomonas syringae

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