In-house validation of an ELISA method for screening of semicarbazide in eggs

Vass, M.; Diblíková, I; Kok, E. A.; Šťastný, Kamil; Frgalová, K.; Hruška, K.; Fránek, Milan

doi:10.1080/02652030701883203

Cited by 18 publications

(18 citation statements)

References 13 publications

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“…Due to the widespread existence of SEM in foodstuffs, there is a sudden concern about SEM safety in recent years. Available experimental data showed SEM possessed carcinogenic potential and can induce genotoxic effects and other toxic effects in the cardiovascular (i.e., aorta) and skeletal systems (Becalski et al 2004;Noonan et al 2008;Vass et al 2008).…”

Section: Introductionmentioning

confidence: 99%

“…Currently, many methods are established for detecting SEM in complicated food samples including enzyme-linked immunosorbent assay (ELISA) (Cháfer-Pericás et al 2010;Cooper et al 2007;Vass et al 2008), immunochromatographic assay (Tang et al 2011) and liquid chromatography tandem mass spectrometry (LC-MS/MS) (Bogialli and Di Corcia 2009;Cháfer-Pericás et al 2010). These methods are accurate and sensitive, but they require complex laboratory equipment, trained personnel, difficultly prepared antibodies, or relatively expensive equipment (e.g., HPLC-MS/MS).…”

Section: Introductionmentioning

confidence: 99%

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A Rapid and Sensitive Method for Semicarbazide Screening in Foodstuffs by HPLC with Fluorescence Detection

Tang

Wang

et al. 2014

Food Anal. Methods

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Semicarbazide (SEM) has been proven to extensively exist in foodstuffs due to anthropogenic factor in food processing and possesses various toxic effects on human health. Although many methods have been developed, they often require long analytical time, complex laboratory equipment, trained personnel, difficultly prepared antibodies, or relatively expensive equipment. The present study developed a new method for SEM determination by HPLC with fluorescence detection (FLD). The fluorescence reagent, 2-(11H-benzo[a]carbazol-11-yl) ethyl chloroformate (BCEC), was first used for SEM labeling. The fluorescent labeling conditions were optimized systematically. SEM can be labeled in only 10 min at 40°C. The labeled SEM was analyzed on an eclipse XDB-C8 column in 8 min. The new method offered the low LOD of 0.4 μg/kg at a signal-to-noise ratio of 3 and also exhibited excellent reproducibility, precision, and accuracy. When applied to analyze several foodstuffs, it showed good applicability. The developed method has been proven to be simple, inexpensive, selective, sensitive, accurate, and reliable for SEM analysis in foodstuffs. Furthermore, this developed method should have a powerful potential in the analysis of SEM from many other food samples.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A Rapid and Sensitive Method for Semicarbazide Screening in Foodstuffs by HPLC with Fluorescence Detection

Tang

Wang

et al. 2014

Food Anal. Methods

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“…ELISAs have been developed for [(2-nitrophenyl) methylene]-semicarbazide (NPSEM) in chicken (Cooper et al, 2007), pork (Vass et al, 2008a) and eggs (Vass et al, 2008b) with CCβ values of 0.25, 0.3 and 0.3 µg/kg, respectively.…”

Section: Screening Methodsmentioning

confidence: 99%

Scientific Opinion on nitrofurans and their metabolites in food

Panel¹

2015

EFS2

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Nitrofurans are antimicrobial agents not authorised for use in food-producing animals in the European Union. Nitrofurans are rapidly metabolised, occurring in animal tissues as protein-bound metabolites. The European Commission requested EFSA to provide a scientific opinion on the risks to human health related to the presence of nitrofurans in food and whether a reference point for action (RPA) of 1.0 µg/kg for the marker metabolites is adequate to protect public health. Data on occurrence of nitrofuran marker metabolites in food were extracted from the national residue monitoring plan results and from the Rapid Alert System for Food and Feed (RASFF). The CONTAM Panel concluded that these data were too limited to carry out a reliable human dietary exposure assessment. Instead, human dietary exposure was calculated for a scenario in which a single nitrofuran marker metabolite is present at 1.0 µg/kg in foods of animal origin, excluding milk and dairy products. The mean chronic dietary exposure for this worst-case scenario would range from 3.3 to 8.0 and 1.9 to 4.3 ng/kg b.w. per day for toddlers and adults, respectively. Nitrofurans and their marker metabolites, generally, are genotoxic and carcinogenic and, also, have non-neoplastic effects in animals. Margins of exposure (MOEs) were calculated at 2.0 × 10 5 or greater for carcinogenicity and at 2.5 × 10 3 or greater for non-neoplastic effects. The CONTAM Panel concluded that it is unlikely that exposure to food contaminated with nitrofuran marker metabolites at or below 1.0 µg/kg is a health concern. A scenario in which foods are considered to be contaminated with semicarbazide, from use of carrageenan as a food additive, at 1 µg/kg was used to assess whether it is appropriate to apply the RPA to foods of non-animal origin; MOEs of greater than 10 4 calculated for nonneoplastic effects do not indicate a health concern.© European Food Safety Authority, 2015 However, the opinion also identified certain categories of non-allowed pharmacologically active substances that are considered to be outside the scope of the procedure, including substances that are high potency carcinogens, such as nitrofurans. As nitrofurans are excluded from that opinion, and taking into account that the presence of SEM in food may be from sources other than use of nitrofurazone, the European Commission (EC) requested the European Food Safety Authority (EFSA) for a scientific opinion on the risks to human health related to the presence of nitrofurans and their metabolites in food. The opinion should include (a) an evaluation of the toxicity of nitrofurans and their metabolites for humans, considering all relevant toxicological endpoints and identification of the toxicological relevance of nitrofurans and their metabolites present in food, and (b) an exposure assessment of the EU population to nitrofurans and their metabolites from food, including the consumption patterns of specific (vulnerable) groups of the population. In addition, the opinion should assess the appropriateness of...

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“…In the meantime several examples of screening validation have been published according to CD criteria. Unfortunately, in absence of detailed information, different strategies and calculations have been applied also borrowing some ideas from the indications suggested for confirmatory methods [9][10][11][12][13][14][15][16][17][18][19][20][21][22][23][24][25].…”

Section: Validation Of Screening Tests According To Commission Decisimentioning

confidence: 99%

“…(2) is strictly linked to the false non compliant rate and therefore its value must be fixed considering the one tailed Student-t tabulated value (t˛, ) corresponding to the desired ␣ error and to the number of experiments carried out to estimate SD blanks (degree of freedom, ). However in the papers reporting the validation of screening tests carried out according to CD (Table 1) the only Threshold [18][19][20]23] LOD [13,21] Decision limit (CC␣) [10,11,15,16,22,24,25] How is established…”

Section: Validation Of Screening Tests According To Commission Decisimentioning

confidence: 99%

Development, validation and data quality assurance of screening methods: A case study

Galarini

Buratti

Fioroni

et al. 2011

Analytica Chimica Acta

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In-house validation of an ELISA method for screening of semicarbazide in eggs

Cited by 18 publications

References 13 publications

A Rapid and Sensitive Method for Semicarbazide Screening in Foodstuffs by HPLC with Fluorescence Detection

A Rapid and Sensitive Method for Semicarbazide Screening in Foodstuffs by HPLC with Fluorescence Detection

Scientific Opinion on nitrofurans and their metabolites in food

Development, validation and data quality assurance of screening methods: A case study

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