In-house validation method for quantification of amoxicillin in medicated feedingstuffs with the use of HPLC-DAD technique

Abstract

<jats:title>Abstract</jats:title><jats:sec id="j_jvetres-2020-0044_s_005_w2aab3b7b1b1b6b1aab1c10b1Aa"><jats:title>Introduction</jats:title><jats:p>A high-performance liquid chromatographic–diode array detector (HPLC-DAD) method for the determination of amoxicillin in medicated feedingstuffs was developed and validated. The method was used to investigate the quality requirements of animal feedingstuffs (declared content of active substance and feed homogeneity).</jats:p></jats:sec><jats:sec id="j_jvetres-2020-0044_s_006_w2aab3b7b1b1b6b1aab1c10b2Aa"><jats:title>Material and Methods</jats:title><jats:p>Two-gram samples were extracted by potassium phosphate buffer solution. Extracts were filtered and directly analysed by HPLC-DAD without further clean-up. Amoxicillin was separated by acetonitrile and 0.01M phosphate buffer (pH 5.0) on a Phenomenex Luna C18 column.</jats:p></jats:sec><jats:sec id="j_jvetres-2020-0044_s_007_w2aab3b7b1b1b6b1aab1c10b3Aa"><jats:title>Results</jats:title><jats:p>This method provided average recoveries of 76.1 to 81.6% with coefficients of variation (CV, %) for repeatability and reproducibility in the ranges of 3.7–7.2% and 5.3–7.6%, respectively. The limit of detection was 51.2 mg/kg and limit of quantification was 103.0 mg/kg.</jats:p></jats:sec><jats:sec id="j_jvetres-2020-0044_s_008_w2aab3b7b1b1b6b1aab1c10b4Aa"><jats:title>Conclusion</jats:title><jats:p>The method was successfully validated and proved to be efficient, precise, and useful for quantification of amoxicillin in medicated feedingstuffs.</jats:p></jats:sec>

Simultaneous determination of sulfonamides, trimethoprim, amoxicillin and tylosin in medicated feed by high performance liquid chromatography with mass spectrometry

“…The presence of interfering substances can be partly eliminated by purifying the sample, but in this case the development of the method requires more work and increases the cost of the analysis. Patyra and Kwiatek ( 18 ) described a method of analysing amoxicillin in medicated feed using the HPLC-DAD technique. They used 0.01 M of phosphate buffer at pH 5.0 and acetonitrile as the mobile phase and gradient elution.…”

“…For tylosin, methanol in citrate buffer ( 20 ), sodium bicarbonate solution with acetonitrile ( 10 ) or a mixture of water and acetonitrile with the addition of formic acid are used ( 20 ). However, antibiotics from the penicillin (amoxicillin, ampicillin and penicillin) class are most often extracted from biological matrices using acetonitrile, an acetonitrile and water mixture or phosphate buffer at different pH ranges ( 1 , 5 , 12 , 18 ). In order to optimise the extraction process, we had to find the all-round best, which meant selecting the solvents which could obtain the highest possible recoveries for all the analysed antibacterial substances.…”

“…In scientific publications, methods can be found of quantitative analysis of single substances in medicated feed ( e.g . sulfaguanidine, tylosin, tiamulin, amoxicillin, florfenicol and sulfamethazine) ( 5 , 10 , 11 , 18 , 20 , 23 , 25 , 26 ), and of analysis of several compounds belonging to the same chemical group (sulfonamides, phenicols, β-lactams and tetracyclines) ( 1 , 2 , 4 , 8 , 12 – 17 , 21 , 22 ). However, few developed analytical methods exist for several antibacterial substances belonging to different chemical groups ( 19 ).…”

Maternal antibiotic treatment affects offspring gastric sensing for umami taste and ghrelin regulation in the pig

“…ANT sows were daily dosed 40 mg/kg body weight of a broad-spectrum antibiotic, amoxicillin (Vetrimoxin PO containing 10% amoxicillin; CEVA Santé Animale, Loudéac, France), together with their morning meal, from day −10 pre-farrowing to day 21 post-farrowing. Among antibiotics approved for use in pigs, amoxicillin was selected because of its large bactericidal spectrum and wide use for group therapies in pigs and poultry [ 31 ] and its frequent use in humans, including babies and pregnant women [ 24 ]. In addition, amoxicillin has a limited gastrointestinal absorption at therapeutic dose because of its saturable transport [ 32 ], therefore, it was supposed to have little direct systemic effects (including in offspring during the period of lactation when the sows of the ANT group received the antibiotic).…”

A Reliable Rp-Uplc-Tuv Method for Simultaneous Estimation of Clarithromycin, Amoxicillin, and Vonoprazan in Co-Packed Pharmaceutical Dosage Forms: Method Development and Validation With Stability Indicating Properties

“…A large number of analytical procedures were observed in the review of literature for analysis of Clarithromycin and Amoxicillin as solo entity and in a mixture with other agents [13][14][15]. In the same way, numerous methods have been pronounced in the literature to estimate Clarithromycin and Amoxicillin combination with other anti-ulcer drugs such as omeprazole, lansoprazole, and rabeprazole [16][17][18][19].…”