In focus in Bad Ischl: Golgi apparatus 2013

Pavelka, Margit; Roth, Jürgen

doi:10.1007/s00418-013-1126-5

Cited by 2 publications

(1 citation statement)

References 14 publications

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“…The Golgi apparatus in mammalian cells is a complex and dynamic organelle that is involved in multiple cellular tasks and built up from interconnected highly organized stacks of cisternae that form a central crossroads in both the secretory and the endocytic pathways (Farquhar and Palade 1981; Mollenhauer and Morré 1991; Berger 1997; Roth 1997; Farquhar and Palade 1998; Lippincott-Schwartz et al 1998; Pavelka et al 2008; Klumperman 2011; Lowe 2011; Nakamura et al 2012; Day et al 2013; Pavelka and Roth 2013; Papanikou and Glick 2014; Rothman 2014). Its structures and functions have been extensively studied, and advanced microscopic methods, including cryo-techniques and correlative light-electron microscopy, together with three-dimensional reconstructions have provided better insights into the complex Golgi architectures and fine structure–function relationships (e.g., Ladinski et al 1999; Grabenbauer et al 2005; Han et al 2013; Marsh and Pavelka 2013; Beznoussenko et al 2014; Engel et al 2015; Koga et al 2016).…”

Section: Introductionmentioning

confidence: 99%

Golgi apparatus dis- and reorganizations studied with the aid of 2-deoxy-d-glucose and visualized by 3D-electron tomography

Ranftler

Meisslitzer-Ruppitsch

Neumüller

et al. 2016

Histochem Cell Biol

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We studied Golgi apparatus disorganizations and reorganizations in human HepG2 hepatoblastoma cells by using the nonmetabolizable glucose analogue 2-deoxy-d-glucose (2DG) and analyzing the changes in Golgi stack architectures by 3D-electron tomography. Golgi stacks remodel in response to 2DG-treatment and are replaced by tubulo-glomerular Golgi bodies, from which mini-Golgi stacks emerge again after removal of 2DG. The Golgi stack changes correlate with the measured ATP-values. Our findings indicate that the classic Golgi stack architecture is impeded, while cells are under the influence of 2DG at constantly low ATP-levels, but the Golgi apparatus is maintained in forms of the Golgi bodies and Golgi stacks can be rebuilt as soon as 2DG is removed. The 3D-electron microscopic results highlight connecting regions that interlink membrane compartments in all phases of Golgi stack reorganizations and show that the compact Golgi bodies mainly consist of continuous intertwined tubules. Connections and continuities point to possible new transport pathways that could substitute for other modes of traffic. The changing architectures visualized in this work reflect Golgi stack dynamics that may be essential for basic cell physiologic and pathologic processes and help to learn, how cells respond to conditions of stress.

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Section: Introductionmentioning

confidence: 99%

Golgi apparatus dis- and reorganizations studied with the aid of 2-deoxy-d-glucose and visualized by 3D-electron tomography

Ranftler

Meisslitzer-Ruppitsch

Neumüller

et al. 2016

Histochem Cell Biol

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Cell biology and medical education—a thematic issue dedicated to Adi Ellinger

Pavelka

2018

Wien Med Wochenschr

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This special themed issue of the Wiener Medizinische Wochenschrift addresses various different topics which have in common that they are all connected with Adi Ellinger in some way. Colleagues of Adi's, who worked with him either recently or many years ago, have been invited to contribute to this issue by submitting a manuscript on one of the topics on which they collaborated. The list of the articles mirrors Adi's different interests and his successful collaborations. It is one of the specific features of this issue that contributions of scientific work on burning cell biology questions are alongside topics that have a central role in the teaching and education of medical students. I am very happy and grateful that all those invited scientists, former or current colleagues, and co-workers of Adi's accepted the invitation to contribute to this issue. Two articles are dedicated to medical education. Rudolf Mallinger contributes with a comprehensive text about the history and actual questions on University and Medicine ("Universität und Medizin"). Details of the reform processes relating to the development of a new curriculum for medical students (MCW) are described in the article by Richard März ("A scientific approach to the reform of a medical curriculum: a personal account of the Vienna experience"). The topics addressed in the other contributions provide actual insights into cellular life and death. Essential cell functions in healthy and diseased cells are highlighted, such as cholesterol metabolism in the article by Clemens Röhrl and Herbert Stangl ("Cholesterol metabolism-physiological regulation and pathophysiological deregulation by the endo

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In focus in Bad Ischl: Golgi apparatus 2013

Cited by 2 publications

References 14 publications

Golgi apparatus dis- and reorganizations studied with the aid of 2-deoxy-d-glucose and visualized by 3D-electron tomography

Golgi apparatus dis- and reorganizations studied with the aid of 2-deoxy-d-glucose and visualized by 3D-electron tomography

Cell biology and medical education—a thematic issue dedicated to Adi Ellinger

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