In-depth transcriptomic analyses of LncRNA and mRNA expression in the hippocampus of APP/PS1 mice by Danggui-Shaoyao-San

Song, Zhenyan; Li, Fuzhou; He, Chunxiang; Yu, Jingping; Li, Ping; Li, Ze; Yang, Miao; Cheng, Shaowu

doi:10.18632/aging.104068

Cited by 12 publications

(16 citation statements)

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“…For thousands of years, traditional Chinese medicine (TCM) has played an irreplaceable role in the Chinese medical system ( 20 ), and TCM has unique advantages in treating complex diseases such as dysmnesia. The holistic view of (TCM) regarding the treatment of multiple components and multiple targets provides a bright prospect for the prevention and treatment of cognitive disorders ( 21 ). TCM can exert its effect by operating in the gut microbiota and is a natural intestinal microecological regulator, and the gut microbiota has emerged as a novel and important field for understanding TCM ( 22 , 23 ).…”

Section: Introductionmentioning

confidence: 99%

Danggui-Shaoyao-San Attenuates Cognitive Impairment via the Microbiota–Gut–Brain Axis With Regulation of Lipid Metabolism in Scopolamine-Induced Amnesia

et al. 2022

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Danggui-Shaoyao-San (DSS) has a long history of being used as a traditional medicine (TCM) and has been reported to show therapeutic effects in alleviating the symptoms of cognitive impairment. The purpose of this study was to investigate whether DSS treatment attenuates cognitive impairment via the microbiota–gut–brain axis in scopolamine-induced amnesia. In this work, we first performed the Morris water maze (MWM) test and novel object recognition (NOR) test to evaluate the memory function of treated C57BL/6N mice. Then we evaluated 16S rRNA for gut microbiota analysis, as well as assessment of blood–brain barrier function and intestinal barrier function and lipid metabolism analysis on tissues from different groups. We hypothesised that DSS may affect brain function and behavior through the gut–brain axis in a bidirectional interplay with both top-down and bottom-up regulation. Furthermore, in order to confirm whether intestinal flora plays a crucial role in scopolamine-induced amnesia, C57BL/6N mice were treated with fecal microbial transplantation (FMT), and then behavioral tests were performed. The mice’s feces were simultaneously evaluated by 16S rRNA analysis. The result supported that the FMT-induced improvement in cognitive function highlights the role of the gut microbiota–brain axis to mediate cognitive function and behavior. Besides theses works, more findings indicated that DSS altered lipid metabolism by activating LXR-PPAR-γ and repaired mucosal barrier dysfunction assessed with a broad range of techniques, which attenuated cognitive impairment via the microbiota–gut–brain axis.

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Section: Introductionmentioning

confidence: 99%

Danggui-Shaoyao-San Attenuates Cognitive Impairment via the Microbiota–Gut–Brain Axis With Regulation of Lipid Metabolism in Scopolamine-Induced Amnesia

et al. 2022

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“…Additionally, hundreds of lncRNAs have been shown to have altered expression in the hippocampus of AD mouse models [209]. Consistent with this, treatment of APP/PS1 mice with Danggui-Shaoyao-San, which improves cognitive functioning with age, leads to differential expression of 285 lncRNAs in the hippocampus [210]. Additionally, one study found increased levels of the lncRNA LoNA in the brain of APP/PS1 mice and observed rescued learning and memory deficits following LoNA knockdown in the hippocampus of APP/PS1 mice compared to controls [76].…”

Section: Epigenetics In Alzheimer's Disease-related Memory Lossmentioning

confidence: 64%

Epigenetic Mechanisms in Memory and Cognitive Decline Associated with Aging and Alzheimer’s Disease

Maity

Farrell

Navabpour

et al. 2021

IJMS

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Epigenetic mechanisms, which include DNA methylation, a variety of post-translational modifications of histone proteins (acetylation, phosphorylation, methylation, ubiquitination, sumoylation, serotonylation, dopaminylation), chromatin remodeling enzymes, and long non-coding RNAs, are robust regulators of activity-dependent changes in gene transcription. In the brain, many of these epigenetic modifications have been widely implicated in synaptic plasticity and memory formation. Dysregulation of epigenetic mechanisms has been reported in the aged brain and is associated with or contributes to memory decline across the lifespan. Furthermore, alterations in the epigenome have been reported in neurodegenerative disorders, including Alzheimer’s disease. Here, we review the diverse types of epigenetic modifications and their role in activity- and learning-dependent synaptic plasticity. We then discuss how these mechanisms become dysregulated across the lifespan and contribute to memory loss with age and in Alzheimer’s disease. Collectively, the evidence reviewed here strongly supports a role for diverse epigenetic mechanisms in memory formation, aging, and neurodegeneration in the brain.

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“…Approximately, 12.6 g of lyophilized powder was prepared from 100 g of raw materials. Ferulic acid, paeoniflorin, ligustilide, atractylodes lactone I, and alisol B 23-acetate were used as standards to identify the main components of DSS for quality control [ 13 ].…”

Section: Methodsmentioning

confidence: 99%

“…The dosages were determined based on body surface area conversion between humans and rats, as well as previous studies. The median and high doses were two and four times the adult dose, respectively [ 13 , 18 ]. The sham operation and model groups were administered an equal volume of normal saline.…”

Section: Methodsmentioning

confidence: 99%

“…Clinical and experimental evidence has demonstrated the therapeutic effects of DSS on AD by inhibiting neuronal damage, providing anti-inflammatory and antioxidant activities, enhancing cognitive behavior, increasing energy metabolism, improving mitochondrial damage, and promoting synaptic formation [7][8][9][10][11]. Our previous network pharmacological and transcriptomic analyses found that the therapeutic mechanism of DSS in AD was closely related to mitochondrial dysfunction and mitophagy [12,13]. Recent research showed that paeoniflorin, an active ingredient of DSS, activated mitophagy to protect SH-SY5Y cells from oxidative damage [14].…”

Section: Introductionmentioning

confidence: 99%

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Neuroprotective Effect of Danggui Shaoyao San via the Mitophagy-Apoptosis Pathway in a Rat Model of Alzheimer’s Disease

Song

Luo

Wang

et al. 2021

Evidence-Based Complementary and Alternative Medicine

Self Cite

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Alzheimer’s disease (AD) is a serious neurodegenerative disease. While the main pathological characteristic of AD is widely believed to be the accumulation of amyloid-beta (Aβ) in neurons around neurofibrillary plaques, the molecular mechanism of pathological changes is not clear. Traditional Chinese medicine offers many treatments for AD. Among these, Danggui Shaoyao San (DSS) is a classic prescription. In this study, an AD model was established by injecting Aβ 1–42 into the brains of rats, which were then treated with different concentrations of Danggui Shaoyao San (sham operation; model; and Danggui Shaoyao San high-dose, medium-dose, and low-dose intervention groups). The Morris water maze test was used to assess the learning and memory abilities of the animals in each group. Nissl staining was used to detect neurons. Mitophagy was evaluated by transmission electron microscopy and immunofluorescence colocalization. Apoptosis was assessed by terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay. The expression levels of autophagy- and apoptosis-related proteins were measured by western blot. Compared to the model group, the groups of AD rats administered medium and high doses of Danggui Shaoyao San showed significantly increased learning and memory abilities ( P < 0.05 ), as well as significantly increased autophagosomes in the hippocampus. Moreover, the expression of PTEN-induced kinase 1 (PINK1), Parkin, and microtubule-associated protein light chain 3 (LC3-I/LC3-II) was increased, while that of p62 was significantly decreased ( P < 0.05 ). The neuronal apoptosis rate was also significantly decreased, the Bcl-2/Bax ratio was significantly increased, and the cleaved caspase-3 protein expression was significantly decreased ( P < 0.05 ). Therefore, Danggui Shaoyao San inhibited neuronal apoptosis in AD rats via a mechanism that may be related to the activation of the PINK1-Parkin-mediated mitophagy signaling pathway.

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In-depth transcriptomic analyses of LncRNA and mRNA expression in the hippocampus of APP/PS1 mice by Danggui-Shaoyao-San

Cited by 12 publications

References 50 publications

Danggui-Shaoyao-San Attenuates Cognitive Impairment via the Microbiota–Gut–Brain Axis With Regulation of Lipid Metabolism in Scopolamine-Induced Amnesia

Danggui-Shaoyao-San Attenuates Cognitive Impairment via the Microbiota–Gut–Brain Axis With Regulation of Lipid Metabolism in Scopolamine-Induced Amnesia

Epigenetic Mechanisms in Memory and Cognitive Decline Associated with Aging and Alzheimer’s Disease

Neuroprotective Effect of Danggui Shaoyao San via the Mitophagy-Apoptosis Pathway in a Rat Model of Alzheimer’s Disease

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