In depth study on thermosensitive liposomes: Optimizing formulations for tumor specific therapy and in vitro to in vivo relations

Lokerse, Wouter J.M.; Kneepkens, Esther; Hagen, Timo L.M. ten; Eggermont, Alexander M.M.; Grüll, Holger; Koning, Gerben A.

doi:10.1016/j.biomaterials.2015.12.023

Cited by 74 publications

(48 citation statements)

References 27 publications

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“…We found that 5-FU release was slow at 37°C (,28%) and exhibited faster drug release (90%) at 39°C, which is characteristic of TDDS, such as thermoresponsive liposomes ( Figure 5B). 8 The significantly higher drug release at 39°C (p-value ,0.05) confirmed that the SLNs show thermoresponsive drug release at hyperthermic temperature. It can also be seen that the drug release at 39°C is similar to the drug release from model OA emulsions used in the study (Figure 5B), thus suggesting that the thermoresponsive drug release from SLNs is due to their existence in the liquid state at hyperthermic temperature.…”

Section: Thermoresponsive Drug Release Studies By Uv-vis Spectrophotomentioning

confidence: 70%

“…1 Many embodiments of thermoresponsive liposomes show low encapsulation efficiency (EE) 6 and unpredictable drug release, 3,7 and their blood circulation life is very short. 1,8 On the other hand, solid lipid nanoparticles (SLNs) have been reported to show long circulation life (24 hours) and passively target cancers by enhanced permeability and retention (EPR) effect. 9,10 In addition to this, SLN composition and their methods of preparation are considered safe as compared to other novel DDS such as liposomes.…”

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confidence: 99%

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Solid lipid nanoparticles for thermoresponsive targeting: evidence from spectrophotometry, electrochemical, and cytotoxicity studies

Rehman¹,

Ihsan²,

Madni³

et al. 2017

IJN

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Thermoresponsive drug delivery systems are designed for the controlled and targeted release of therapeutic payload. These systems exploit hyperthermic temperatures (>39°C), which may be applied by some external means or due to an encountered symptom in inflammatory diseases such as cancer and arthritis. The objective of this paper was to provide some solid evidence in support of the hypothesis that solid lipid nanoparticles (SLNs) can be used for thermoresponsive targeting by undergoing solid–liquid phase transition at their melting point (MP). Thermoresponsive lipid mixtures were prepared by mixing solid and liquid natural fatty acids, and their MP was measured by differential scanning calorimetry (DSC). SLNs (MP 39°C) containing 5-fluorouracil (5-FU) were synthesized by hot melt encapsulation method, and were found to have spherical shape (transmission electron microscopy studies), desirable size (<200 nm), and enhanced physicochemical stability (Fourier transform infrared spectroscopy analysis). We observed a sustained release pattern (22%–34%) at 37°C (5 hours). On the other hand, >90% drug was released at 39°C after 5 hours, suggesting that the SLNs show thermoresponsive drug release, thus confirming our hypothesis. Drug release from SLNs at 39°C was similar to oleic acid and linoleic acid nanoemulsions used in this study, which further confirmed that thermoresponsive drug release is due to solid–liquid phase transition. Next, a differential pulse voltammetry-based electrochemical chemical detection method was developed for quick and real-time analysis of 5-FU release, which also confirmed thermoresponsive drug release behavior of SLNs. Blank SLNs were found to be biocompatible with human gingival fibroblast cells, although 5-FU-loaded SLNs showed some cytotoxicity after 24 hours. 5-FU-loaded SLNs showed thermoresponsive cytotoxicity to breast cancer cells (MDA-MB-231) as cytotoxicity was higher at 39°C (cell viability 72%–78%) compared to 37°C (cell viability >90%) within 1 hour. In conclusion, this study presents SLNs as a safe, simple, and effective platform for thermoresponsive targeting.

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Section: Thermoresponsive Drug Release Studies By Uv-vis Spectrophotomentioning

confidence: 70%

mentioning

confidence: 99%

Solid lipid nanoparticles for thermoresponsive targeting: evidence from spectrophotometry, electrochemical, and cytotoxicity studies

Rehman¹,

Ihsan²,

Madni³

et al. 2017

IJN

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“…The last but not the least, they can increase drug bioavailability and the fraction of the drug accumulated in the pathological area. A variety of drug delivery and drug targeting systems, such as synthetic polymers(Chenna et al, 2012; Usacheva et al, 2014; Kumar et al, 2015), microcapsules (Chen et al, 2015), lipoproteins (Helbok et al, 2012; Shen et al, 2016), liposomes (Yuan et al, 2013; Han et al, 2014; Lokerse et al, 2016), lipid particles(You et al, 2015), and many others have been designed and exploited for cancer therapy (Torchilin, 2006). Therefore, they hold great potential to generate practical strategies for the CSC therapy in the near future.…”

Section: Organic Nanoparticles For Targeting Cscsmentioning

confidence: 99%

Nanomaterials in Targeting Cancer Stem Cells for Cancer Therapy

et al. 2017

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Cancer stem cells (CSCs) have been identified in almost all cancers and give rise to metastases and can also act as a reservoir of cancer cells that may cause a relapse after surgery, radiation, or chemotherapy. Thus they are obvious targets in therapeutic approaches and also a great challenge in cancer treatment. The threat presented by CSCs lies in their unlimited proliferative ability and multidrug resistance. These findings have necessitated an effective novel strategy to target CSCs for cancer treatment. Nanomaterials are on the route to providing novel methods in cancer therapies. Although, there have been a large number of excellent work in the field of targeted cancer therapy, it remains an open question how nanomaterials can meet future demands for targeting and eradicating of CSCs. In this review, we summarized recent and highlighted future prospects for targeting CSCs for cancer therapies by using a variety of nanomaterials.

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“…and thus provide the ability to be transferred to a wide range of applications. Depending on the carrier formulation, the release of the therapeutic agent can be obtained either by mechanical [34] or thermal effect [35,36]. Extensive and recent analyses of these promising strategies can be found [37,38].…”

Section: Mechanisms Of Ultrasound-mediated Drug Deliverymentioning

confidence: 98%

Ultrasound-mediated ocular delivery of therapeutic agents: a review

Lafond

Aptel

Mestas

et al. 2016

Expert Opinion on Drug Delivery

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Introduction: Due to numerous anatomical and physiological barriers, ocular drug delivery remains a major limitation in the treatment of diseases such as glaucoma, macular degeneration or inflammatory diseases. To date, only invasive approaches provide clinically effective results. Ultrasound can be defined as the propagation of a high-frequency sound wave exposing the propagation media to mechanical and thermal effects. Ultrasound has been proposed as a non-invasive physical agent for increasing therapeutic agent delivery in various fields of medicine.Areas Covered: An update on recent advances in transscleral and transcorneal ultrasoundmediated drug delivery is presented. Efficient drug delivery is achieved in vitro, ex vivo and in vivo for various types of materials. Numerous studies indicate that efficacy is related to Downloaded by [University of Lethbridge] at 02:55 18 June 2016 2 cavitation. Although slight reversible effects can be observed on the corneal epithelium, efficient drug delivery can be performed without causing damage to the cornea. Expert Opinion: Recent developments prove the potential of ultrasound-mediated ocular drug delivery. Cavitation appears to be a preponderant mechanism, opening a way to treatment monitoring by cavitation measurement. Even if no clinical studies have yet been performed, the promising results summarized here are promoting developments toward clinical applications, particularly in assessing the safety of the technique. Article highlights box• Ocular drug delivery limits the treatment of numerous diseases because of anatomical and physiological barriers so that current techniques are either inefficient (5% of the dose reaching the interior of the eye) or invasive (intravitreal injections, implants).• Ultrasound offers the possibility of acting noninvasively on the sclera or cornea.• Both in vitro and in vivo experiments report an increase in transscleral and transcorneal drug delivery by applying ultrasound which could suggest an increased treatment efficacy.• The mechanism of enhanced drug delivery is attributable to cavitation, producing slight, reversible changes in tissues.• Slight reversible damages attributable could be found. Thus, with an efficient cavitation monitoring procedure, translation to the clinic should be encouraged, as no severe damage has been observed. Downloaded by [University of Lethbridge] at 02:55 18 June 2016 3• The strategy of dosing the ultrasound exposure on measured cavitation activity in relation with its calibrated bioeffects in terms of damages and efficacy is proposed.Also, future developments shall benefit from the experience of sonophoresis.

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In depth study on thermosensitive liposomes: Optimizing formulations for tumor specific therapy and in vitro to in vivo relations

Cited by 74 publications

References 27 publications

Solid lipid nanoparticles for thermoresponsive targeting: evidence from spectrophotometry, electrochemical, and cytotoxicity studies

Solid lipid nanoparticles for thermoresponsive targeting: evidence from spectrophotometry, electrochemical, and cytotoxicity studies

Nanomaterials in Targeting Cancer Stem Cells for Cancer Therapy

Ultrasound-mediated ocular delivery of therapeutic agents: a review

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