In-depth serum proteomics reveals biomarkers of psoriasis severity and response to traditional Chinese medicine

Xu, Meng; Deng, Jingwen; Xu, Kaikun; Zhu, Tiansheng; Han, Ling; Yan, Yaohua; Yao, Danni; Deng, Hao; Wang, Dan; Sun, Yaoting; Chang, Cheng; Zhang, Xiaomei; Dai, Jiayu; Liang, Yue; Zhang, Qiushi; Cai, Xue; Zhu, Yi; Duan, Haibao; Liu, Yuan; Liu, Dong; Zhu, Ying–Hui; Radstake, Timothy R D J; Balak, Deepak M W; Xu, Danke; Guo, Tiannan; Lu, Chuanjian; Yu, Xiaobo

doi:10.7150/thno.31144

Cited by 89 publications

(93 citation statements)

References 76 publications

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“…Using these reference sequences, we prepared a peptide library containing 966 peptides representing SARS-CoV-2 proteins, in which each peptide was 15 amino acids long with a 5 amino acid overlap. All peptides were labeled with a C-terminal biotin group and printed onto a three-dimensional (3D) modified microscope slide using biotin–streptavidin chemistry, 21 such that the peptides were immobilized on the slide via their C-terminus. Full-length SARS-CoV-2 N protein, full-length E, and five S truncated proteins were also printed ( Supporting Information Table 1 ).…”

Section: Resultsmentioning

confidence: 99%

SARS-CoV-2 Proteome Microarray for Mapping COVID-19 Antibody Interactions at Amino Acid Resolution

et al. 2020

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Comprehensive profiling of humoral antibody response to severe acute respiratory syndrome (SARS) coronavirus-2 (CoV-2) proteins is essential in understanding the host immunity and in developing diagnostic tests and vaccines. To address this concern, we developed a SARS-CoV-2 proteome peptide microarray to analyze antibody interactions at the amino acid resolution. With the array, we demonstrate the feasibility of employing SARS-CoV-1 antibodies to detect the SARS-CoV-2 nucleocapsid phosphoprotein. The first landscape of B-cell epitopes for SARS-CoV-2 IgM and IgG antibodies in the serum of 10 coronavirus disease of 2019 (COVID-19) patients with early infection is also constructed. With array data and structural analysis, a peptide epitope for neutralizing antibodies within the SARS-CoV-2 spike receptor-binding domain’s interaction interface with the angiotensin-converting enzyme 2 receptor was predicted. All the results demonstrate the utility of our microarray as a platform to determine the changes of antibody responses in COVID-19 patients and animal models as well as to identify potential targets for diagnosis and treatment.

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Section: Resultsmentioning

confidence: 99%

SARS-CoV-2 Proteome Microarray for Mapping COVID-19 Antibody Interactions at Amino Acid Resolution

et al. 2020

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“…The antibody microarrays and sera screening were processed as previously described (10). Briefly, 10 μL of serum were diluted with 90 μL phosphate buffered saline (PBS; pH 7.4) and labeled with 1 μL of NHS-PEG4-Biotin (20 After removing the excess biotin molecules, the biotinylated proteins were dissolved in 400 μL of PBS containing 5% milk (w/v).…”

Section: Screening Of Covid-19 Serum Proteome Using Antibody Microarraysmentioning

confidence: 99%

“…As of May 04, 2020, 3 (8,9). However, to date, there is neither a vaccine to prevent COVID-19 nor an effective medicine to treat In this study, a comprehensive analysis of serological proteins from patients in the early stages of COVID-19 or influenza was performed using an antibody microarray developed in our laboratory (10). A large number of differentiallyexpressed proteins were identified that mediates diverse immune signaling by guest on November 3, 2020 pathways related to viral infection, the inflammatory response, and immune cell activation and migration.…”

Section: Introductionmentioning

confidence: 99%

Serum Protein Profiling Reveals a Landscape of Inflammation and Immune Signaling in Early-stage COVID-19 Infection

Hou

Zhang

et al. 2020

Molecular & Cellular Proteomics

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Coronavirus disease 2019 (COVID-19) is a highly contagious infection and threating the human lives in the world. The elevation of cytokines in blood is crucial to induce cytokine storm and immunosuppression in the transition of severity in COVID-19 patients. However, the comprehensive changes of serum proteins in COVID-19 patients throughout the SARS-CoV-2 infection is unknown. In this work, we developed a high-density antibody microarray and performed an in-depth proteomics analysis of serum samples collected from early COVID-19 (n=15) and influenza (n=13) patients. We identified a large set of differentially expressed proteins (n=132) that participate in a landscape of inflammation and immune signaling related to the SARS-CoV-2 infection. Furthermore, the significant correlations of neutrophil and lymphocyte with the CCL2 and CXCL10 mediated cytokine signaling pathways was identified. These information are valuable for the understanding of COVID-19 pathogenesis, identification of biomarkers and development of the optimal anti-inflammation therapy.

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“…e unknown pathogenesis has brought difficulties to the treatment, without curative approaches against psoriasis at present. Traditional Chinese medicine (TCM) has unique advantages in treating psoriasis, which can play therapeutic roles through multiple targets and multiple pathways, corresponding to the dysfunction of various pathways underlying the pathogenesis of psoriasis [10][11][12][13]. However, TCM also has defects.…”

Section: Introductionmentioning

confidence: 99%

Investigation on the Mechanism of Qubi Formula in Treating Psoriasis Based on Network Pharmacology

Zhou

Zhang²,

Tao

2020

Evidence-Based Complementary and Alternative Medicine

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Objective. To elucidate the pharmacological mechanisms of Qubi Formula (QBF), a traditional Chinese medicine (TCM) formula which has been demonstrated as an effective therapy for psoriasis in China. Methods. The Traditional Chinese Medicine Systems Pharmacology (TCMSP) database, BATMAN-TCM database, and literature search were used to excavate the pharmacologically active ingredients of QBF and to predict the potential targets. Psoriasis-related targets were obtained from Therapeutic Target Database (TTD), DrugBank database (DBD), MalaCards database, and DisGeNET database. Then, we established the network concerning the interactions of potential targets of QBF with well-known psoriasis-related targets by using protein-protein interaction (PPI) data in String database. Afterwards, topological parameters (including DNMC, Degree, Closeness, and Betweenness) were calculated to excavate the core targets of Qubi Formula in treating psoriasis (main targets in the PPI network). Cytoscape was used to construct the ingredients-targets core network for Qubi Formula in treating psoriasis, and ClueGO was used to perform GO-BP and KEGG pathway enrichment analysis on these core targets. Results. The ingredient-target-disease core network of QBF in treating psoriasis was screened to contain 175 active ingredients, which corresponded to 27 core targets. Additionally, enrichment analysis suggested that targets of QBF in treating psoriasis were mainly clustered into multiple biological processes (associated with nuclear translocation of proteins, cellular response to multiple stimuli (immunoinflammatory factors, oxidative stress, and nutrient substance), lymphocyte activation, regulation of cyclase activity, cell-cell adhesion, and cell death) and related pathways (VEGF, JAK-STAT, TLRs, NF-κB, and lymphocyte differentiation-related pathways), indicating the underlying mechanisms of QBF on psoriasis. Conclusion. In this work, we have successfully illuminated that Qubi Formula could relieve a wide variety of pathological factors (such as inflammatory infiltration and abnormal angiogenesis) of psoriasis in a “multicompound, multitarget, and multipathway” manner by using network pharmacology. Moreover, our present outcomes might shed light on the further clinical application of QBF on psoriasis treatment.

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In-depth serum proteomics reveals biomarkers of psoriasis severity and response to traditional Chinese medicine

Cited by 89 publications

References 76 publications

SARS-CoV-2 Proteome Microarray for Mapping COVID-19 Antibody Interactions at Amino Acid Resolution

SARS-CoV-2 Proteome Microarray for Mapping COVID-19 Antibody Interactions at Amino Acid Resolution

Serum Protein Profiling Reveals a Landscape of Inflammation and Immune Signaling in Early-stage COVID-19 Infection

Investigation on the Mechanism of Qubi Formula in Treating Psoriasis Based on Network Pharmacology

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