In Depth Quantitative Proteomic and Transcriptomic Characterization of Human Adipocyte Differentiation using the SGBS Cell Line

Abstract

Most information on molecular processes accompanying and driving adipocyte differentiation are derived from rodent models. Here, a comprehensive analysis of combined transcriptomic and proteomic alterations during adipocyte differentiation in Simpson–Golabi–Behmel Syndrome (SGBS) cells is provided. The SGBS cells are a well‐established and the most widely applied cell model to study human adipocyte differentiation and cell biology. However, the molecular alterations during human adipocyte differentiation in SGBS cells have not yet been described in a combined analysis of proteome and transcriptome. Here a global proteomic and transcriptomic data set comprising relative quantification of a total of 14372 mRNA transcripts and 2641 intracellular and secreted proteins is presented. 1153 proteins and 313 genes are determined as differentially expressed between preadipocytes and the fully differentiated cells including adiponectin, lipoprotein lipase, fatty acid binding protein 4, fatty acid synthase, stearoyl‐CoA desaturase, and apolipoprotein E and many other proteins from the fatty acid synthesis, amino acid synthesis as well as glucose and lipid metabolic pathways. Preadipocyte markers, such as latexin, GATA6, and CXCL6, are found to be significantly downregulated at the protein and transcript level. This multi‐omics data set provides a deep molecular profile of adipogenesis and will support future studies to understand adipocyte function.

Comparative Proteomic Profiling of 3T3-L1 Adipocyte Differentiation Using SILAC Quantification

“…This study helps our understanding of the complicated metabolic events that occur in relation to the changes in metabolic enzymes involved in adipocyte differentiation. Previous proteomics studies on adipogenesis have reported that PPARγ signaling, TCA cycle, lipid metabolism, fatty acid β-oxidation metabolism, electron transport chain, and redox system were up-regulated in adipocyte differentiation. − Our proteomic results are confirmatory of these previous findings. In addition, we identified additional proteins such as those involved in nicotinamide metabolism, those that induced changes in cristae and mitochondrial protein import, and in the transport of Ca 2+ into mitochondria and ER.…”

“…Similar to our study, a recent SILAC based-quantitative proteomics investigation of human adipocytes has reported that adipogenic markers and many other proteins from the lipid metabolic pathways are up-regulated during differentiation of the human Simpson−Golabi−Behmel syndrome (SGBS) adipocytes. 78 Journal of Proteome Research Proteins annotated in neXtProt knowledgebase, inclusive of PE, splice isoform, and uPE, are subject for a future study in order to verify their expression and potential functions in adipocytes.…”

Exosomal miR-1304-3p promotes breast cancer progression in African Americans by activating cancer-associated adipocytes

“…4e). This finding is consistent with previous data showing that TG and DG are the main components of SGBS lipid contents during differentiation 18 . Notably, 17 out of the 19 top differentially expressed TG molecules contain C18:1 (Fig.…”

Obesity III: Obesogen assays: Limitations, strengths, and new directions

“…suggesting that this cell line might be useful for assessments of adipocyte browning. Proteomic and transcriptomic analyses of SGBS cells have been used to evaluate the molecular underpinnings of SGBS differentiation, with >1100 proteins and >300 genes differentially expressed in differentiated cells relative to undifferentiated [58]. However, some research comparing this model to existing models has suggested notable differences.…”