In‐depth proteome of perilymph in guinea pig model

Abstract

The vast majority of sensorineural hearing loss is caused by impairment of the inner ear cells. Proteomic analysis of perilymph may therefore improve our understanding of inner ear diseases and hearing loss. However, the investigation of the human perilymph proteome was limited due to technical difficulties in perilymph sampling. The guinea pig (Cavia porcellus) is frequently used as an experimental model in preclinical hearing research. In this study, we analyzed samples of perilymph collected from 12 guinea pigs to overcome limited experimental information regarding its proteome. We identified a total of 1413 proteins, establishing a greatly expanded proteome of the previously inferred guinea pig perilymph. This provides a comprehensive proteomic resource for the research community, which will facilitate future molecular‐phenotypic studies using the guinea pig as an experimental model of relevance to human inner ear biology.

Intratympanic steroid treatment can reduce ROS and immune response in human perilymph investigated by in‐depth proteome analysis

“…Human perilymph sample preparation in the IT steroid‐treated group ( n = 19) and non‐treated group ( n = 9) was performed as described previously, with some modifications [5]. Samples were collected from nine individuals each from the IT steroid‐treated group and the non‐treated group, and the perilymph sample was extracted from either side of the inner ear.…”

“…LC‐MS/MS analysis was performed using an Ultimate 3000 RSLC system (Dionex, USA) connected to a Q‐Exactive Plus mass spectrometer (Thermo Scientific, Bremen, Germany) with nano‐electrospray [5]. Briefly, the peptide samples were separated using a two‐column system with a trap column (300 μm I.D.…”

“…Human body fluids contain numerous disease‐associated proteins that are secreted or leaked from pathological tissues throughout the body [8]. In our recent study on animal models, we detected perilymph proteins in extracellular compartments with a likely origin in the inner ear tissues [5]. Compared with other bodily fluids such as plasma, urine, or cerebrospinal fluid, perilymph is more likely to collect proteins from one or more tissue types.…”

“…Despite the wide use of IT steroids to treat hearing loss, the mechanism and biological effects of IT steroids have not been thoroughly studied. Several animal studies have elucidated the proteomic characteristics of the inner ear fluid, perilymph [5]. However, studies are limited, and little is known about the changes in perilymph after IT steroid therapy.…”

Effect and Biocompatibility of a Cross-Linked Hyaluronic Acid and Polylactide-co-glycolide Microcapsule Vehicle in Intratympanic Drug Delivery for Treating Acute Acoustic Trauma

“…Target-oriented local delivery methods such as intratympanic (IT) injection of dexamethasone (D) have been introduced as alternative methods. Due to direct absorption through the round window membrane (RWM), IT injection can achieve a higher concentration of steroid in the perilymph compared with systemic administration [ 7 , 8 , 9 , 10 , 11 ]. A systematic review showed that IT steroids can be a valuable solution for patients with acute SNHL who either cannot tolerate systemic steroid therapy or do not respond to treatment [ 12 ].…”