In-depth proteome analysis reveals multiple pathways involved in tomato SlMPK1-mediated high-temperature responses

Integrated proteome and transcriptome analyses revealed key factors involved in tomato (Solanum lycopersicum) under high temperature stress

“…Interestingly, 10 DEPs (PsbP, PsbQ, PsbS, PsaA, PsaB, PsaF, PsaL, PetB, PetF, b) were significantly enriched in the pathway of “photosynthesis (ko00195),” and all of them were induced by HT except PsbP (Figure S5). The previous study showed that HT‐resistant plants can maintain high photosynthetic capacity against HT damage (Ding et al., 2019). All together, these data suggested that there is no significantly shared response between DEGs and DEPs in terms of photosynthesis pathways.…”

“…For the integration of transcriptome and proteomic data, we dig deeper into the data we have obtained (Ding et al, 2018(Ding et al, , 2019. Here, for quantitative changes, any proteins changed with unique peptide numbers ≥ 1, the cutoff > 1.2-fold or <0.67fold difference, and a p-value < .05 in anyone 8-plex iTRAQ were considered to be statistically different from unity.…”

“…We performed SlMPK1-mediated proteomic signaling pathway analysis. However, we did not conduct in-depth analysis of the HT-responsive proteome (Ding et al, 2018(Ding et al, , 2019. In order to integrate the analyses of proteome and transcriptome data and to meet the large number of HT-responsive DEGs, we analyzed the raw data of the iTRAQ in depth, and there were 2,297 proteins (peptides ≥ 1) overlap in both 8-plex iTRAQ (Figure 1c), indicating the high reliability of our data.…”

Proteomics of Reproductive Development, Fruit Ripening, and Stress Responses in Tomato

“…Thermotolerant tomatoes also displayed increased abundance of proteins that were associated with heat tolerance, such as glutamine synthetase, S -adenosine methionine synthase, and polyphenol oxidases . Many other cytosolic proteins were also reported to be regulated by MAPK signaling, such as SlMPK, which regulates a plethora of proteins related to photosynthesis, the oxidation–reduction process, amino acid biosynthesis, protein folding, and proteolytic degradation under high-temperature stress …”

“…149 Many other cytosolic proteins were also reported to be regulated by MAPK signaling, such as SlMPK, which regulates a plethora of proteins related to photosynthesis, the oxidation−reduction process, amino acid biosynthesis, protein folding, and proteolytic degradation under high-temperature stress. 150 4.1.5. Drought Stress.…”