In Depth Natural Product Discovery from the Basidiomycetes Stereum Species

Tian, Mengqing; Zhao, Pei‐Ji; Li, Guohong; Zhang, Ke‐Qin

doi:10.3390/microorganisms8071049

Cited by 13 publications

(11 citation statements)

References 111 publications

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“…On the other hand, the fungal collection was made up of wood-decaying basidiomycetes. Besides the well-known ability of wood-decaying fungi to control bacterial communities [ 38 , 39 ], the fungi have been chosen for their potential to produce antimicrobial products [ 40 , 41 , 42 , 43 , 44 ], for instance the genera Trametes [ 45 ] or Stereum [ 46 ]. In our experimental design, bacterial and fungal couples were grown on agar plates at each side of the petri dishes ( Figure 1 A) generating a Streptomyces -fungus interaction zone (SFIZ).…”

Section: Resultsmentioning

confidence: 99%

Elicitation of Antimicrobial Active Compounds by Streptomyces-Fungus Co-Cultures

et al. 2021

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The bacteria of the genus Streptomyces and Basidiomycete fungi harbor many biosynthetic gene clusters (BGCs) that are at the origin of many bioactive molecules with medical or industrial interests. Nevertheless, most BGCs do not express in standard lab growth conditions, preventing the full metabolic potential of these organisms from being exploited. Because it generates biotic cues encountered during natural growth conditions, co-culture is a means to elicit such cryptic compounds. In this study, we explored 72 different Streptomyces-fungus interaction zones (SFIZs) generated during the co-culture of eight Streptomyces and nine fungi. Two SFIZs were selected because they showed an elicitation of anti-bacterial activity compared to mono-cultures. The study of these SFIZs showed that co-culture had a strong impact on the metabolic expression of each partner and enabled the expression of specific compounds. These results show that mimicking the biotic interactions present in this ecological niche is a promising avenue of research to explore the metabolic capacities of Streptomyces and fungi.

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Section: Resultsmentioning

confidence: 99%

Elicitation of Antimicrobial Active Compounds by Streptomyces-Fungus Co-Cultures

et al. 2021

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“…Triterpenoids are secondary metabolites of plants, fungi, marine invertebrates, and algae that are formed during cyclization of an acyclic triterpene squalene [ 132 , 133 , 134 , 135 , 136 , 137 , 138 ]. According to the number of cycles, triterpenes and triterpenoids are divided into several groups; the most numerous are pentacyclic triterpenic derivatives [ 139 ].…”

Section: Discussionmentioning

confidence: 99%

Biotransformation of Oleanane and Ursane Triterpenic Acids

2020

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Oleanane and ursane pentacyclic triterpenoids are secondary metabolites of plants found in various climatic zones and regions. This group of compounds is highly attractive due to their diverse biological properties and possible use as intermediates in the synthesis of new pharmacologically promising substances. By now, their antiviral, anti-inflammatory, antimicrobial, antitumor, and other activities have been confirmed. In the last decade, methods of microbial synthesis of these compounds and their further biotransformation using microorganisms are gaining much popularity. The present review provides clear evidence that industrial microbiology can be a promising way to obtain valuable pharmacologically active compounds in environmentally friendly conditions without processing huge amounts of plant biomass and using hazardous and expensive chemicals. This review summarizes data on distribution, microbial synthesis, and biological activities of native oleanane and ursane triterpenoids. Much emphasis is put on the processes of microbial transformation of selected oleanane and ursane pentacyclic triterpenoids and on the bioactivity assessment of the obtained derivatives.

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“…The drugs derived from secondary plant metabolites, including pentacyclic triterpenoids, make up about 25% of the total pharmaceutical market [1]. In nature, pentacyclic triterpenoids are represented by more than 20 structures formed by cyclization of their precursor squalene in the tissues of plants, fungi, marine invertebrates, and algae [2][3][4][5]. One of the most common plant pentacyclic triterpenoids is oleanolic acid (OA; Figure 1), which concentration in fruits and leaves of Olea europaeacan reach 8th Scientific and Practical Conference 35 mg/g dry weight [6,7].…”

Section: Introductionmentioning

confidence: 99%

Features of the Bioconversion of Pentacyclic Triterpenoid Oleanolic Acid Using Rhodococcus Actinobacteria

Luchnikova

Maltseva

Гришко

et al. 2022

KLS

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The ability of actinobacteria of the genus Rhodococcus to transform oleanolic acid (OA), a plant pentacyclic triterpenoid, was shown for the first time using bioresources of the Regional Specialized Collection of AlkanotrophicMicroorganisms (IEGM; WDCM #768;www.iegmcol.ru). The most promising strains (R.opacus IEGM 488 and R.rhodochrousIEGM 285) were selected, and these catalyzed80% bioconversion of OA (0.5 g/L) in the presence of n-hexadecane (0.1% v/v) for seven days. The process of OA bioconversion was accompanied by a gradual decrease in the culture medium pH. Adaptive responses of bacterial cells to the OA effects included the formation of compact cellular aggregates, a marked change in the surface-to-volume ratio of cells, and a significant increase in the Zeta potential values. The results demonstrated that the process of OA bioconversion was catalyzed by membrane-bound enzyme complexes. Participation of cytochrome P450-dependent monooxygenases in the oxidation of the OA moleculewas confirmedusing specific inhibitors. The obtained data expand our knowledge on the catalytic activity of actinobacteria of the genus Rhodococcus and their possible use as biocatalysts for the bioconversion of complex hydrophobic compounds. The results can also be used inthe searchfor promising OA derivatives to be used in the synthesis of biologically active agents. Keywords: bioconversion, oleanolic acid, Rhodococcus, biologically active compounds

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In Depth Natural Product Discovery from the Basidiomycetes Stereum Species

Cited by 13 publications

References 111 publications

Elicitation of Antimicrobial Active Compounds by Streptomyces-Fungus Co-Cultures

Elicitation of Antimicrobial Active Compounds by Streptomyces-Fungus Co-Cultures

Biotransformation of Oleanane and Ursane Triterpenic Acids

Features of the Bioconversion of Pentacyclic Triterpenoid Oleanolic Acid Using Rhodococcus Actinobacteria

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