In-depth mapping carboxylic acid metabolome reveals the potential biomarkers in colorectal cancer through characteristic fragment ions and metabolic flux

Qian, Yun; Tan, Binbin; Li, Kai; Hong, Xufen; Wong, Chi Chun; Fu, Li; Zhang, Jun; Li, Na; Wu, Jian‐Lin

doi:10.1016/j.aca.2020.06.064

Cited by 22 publications

(20 citation statements)

References 23 publications

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“…However, among the 100 compounds, not all of them produced the above two fragments at the same time. A previous study indicated that the identification based on only one fragmentation will lead to serious false-positive results ( Bian et al, 2020 ). Therefore, the fragments after the neutral loss of 179.0946 Da were added for defining carboxyl compounds.…”

Section: Resultsmentioning

confidence: 99%

“…Some efforts have been made to solve the above problems. For example, to achieve high-coverage identification of carboxyl compounds without heavy isotope-labeled derivatization reagents, three characteristic fragment ions from the derivatization group were hunted, and 1054 carboxylic acids were defined after derivatized by 5-(diisopropylamino) amylamine ( Bian et al, 2020 ). The predicted t R was also used for the identification of carboxyl-containing compounds in plasma and yeast ( Zhao, Li, Han, Chan, & Li, 2019 ).…”

Section: Introductionmentioning

confidence: 99%

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In-depth profiling of carboxyl compounds in Chinese Baijiu based on chemical derivatization and ultrahigh-performance liquid chromatography coupled to high-resolution mass spectrometry

et al. 2022

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

In-depth profiling of carboxyl compounds in Chinese Baijiu based on chemical derivatization and ultrahigh-performance liquid chromatography coupled to high-resolution mass spectrometry

et al. 2022

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“…We noticed that several metabolism related terms were significantly enriched, including secondary metabolic process, apical part of cell and carboxylic acid binding GO terms and the steroid hormone biosynthesis pathway. The carboxylic acid is an important substance in the generation and progress of cancers [ 34 ]. Besides, the biosynthesis of steroid hormone, a process requiring multiple enzymes to coordinate [ 35 ], is also found to be related to the formation and growth of prostate cancer [ 36 ].…”

Section: Discussionmentioning

confidence: 99%

“…F-H Kaplan-Meier survival curves of patients in N0, N1, N2-N3 subgroups, separately the steroid hormone biosynthesis pathway. The carboxylic acid is an important substance in the generation and progress of cancers [34]. Besides, the biosynthesis of steroid hormone, a process requiring multiple enzymes to coordinate [35], is also found to be related to the formation and growth of prostate cancer [36].…”

Section: Discussionmentioning

confidence: 99%

A prognostic Risk Score model for oral squamous cell carcinoma constructed by 6 glycolysis-immune-related genes

Liu

Wang

2022

BMC Oral Health

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Background Oral squamous cell carcinoma (OSCC) is the most frequent tumor of the head and neck. The glycolysis-related genes and immune-related genes have been proven prognostic values in various cancers. Our study aimed to test the prognostic value of glycolysis-immune-related genes in OSCC. Methods Data of OSCC patients were obtained from the Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases. Enrichment analysis was applied to the glycolysis- and immune-related genes screened by differential expression analysis. Univariate Cox and LASSO Cox analyses were used to filtrate the genes related to the prognosis of OSCC and to construct Risk Score model. Results A Risk Score model was constructed by six glycolysis-immune-related genes (including ALDOC, VEGFA, HRG, PADI3, IGSF11 and MIPOL1). High risk OSCC patients (Risk Score >−0.3075) had significantly worse overall survival than that of low risk patients (Risk Score <−0.3075). Conclusions The Risk Score model constructed basing on 6 glycolysis-immune-related genes was reliable in stratifying OSCC patients with different prognosis.

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“…Метаболомика позволяет количественно измерить динамический многопараметрический метаболический ответ живых систем на патофизиологические стимулы или генетическую модификацию. Эта технология широко использовалась для идентификации биомаркеров на основе метаболитов при различных формах рака [5][6][7].…”

Section: лабораторные и экспериментальные исследованияunclassified

Fatty acids of erythrocyte membranes and blood serum as biomarkers for early detection of colorectal cancer

et al. 2022

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The aim of the study was to identify fatty acids (FA) of erythrocyte membranes and blood serum, which can serve as potential biomarkers for early detection of colorectal cancer.Material and methods. the study involved 65 patients with stage I-II CRC (mean age 63,3 ± 9,6 years (30 men, 35 women) and 35 people in the comparison group, matched by age and sex. the composition of fatty acids of erythrocyte membranes and blood serum was studied using a gc/ms system based on three agilent 7000B quadrupoles (USA). methods of the MATLAB software (R2019a, MathWorks) and the R programming language were used for statistical processing: t-test, unsupervised principal component analysis (PCA), Fold change, Volcano plot, machine learning method (Random Forest), Roc analysis, Heatmaps.Results. patients with stage I-II CRC showed a decrease in the level of saturated, individual monounsaturated fatty acids and a highly significant increase in the most of the polyunsaturated fatty acids with a predominance of omega-3. For most of the fatty acid levels, significant differences in erythrocyte membranes and serum between the groups were found. the levels of erythrocyte myristic, pentadecanoic, 7-palmitoleic, saturated/polyunsaturated Fa ratios (the content of which was significantly lower at stages I-II CRC than those in the control) and the levels of α-linolenic, eicosapentaenoic, docosapentaenoic, docosahexaenoic, the amount of omega-3 Fa, EPA + DHA, docodienic, dihomo-γ-linolenic, docatetraenoic fatty acids (the content of which was significantly higher in colorectal cancer than those in healthy subjects) were the most discriminating parameters in distinguishing patients with I-II stage CRC and healthy individuals. in the blood serum, the level of biomarkers had the following fatty acids: arachidonic, myristic, docosahexaenoic, the amount of omega 3 puFa, the ratio of omega 6/omega 3 puFa, pentadecanoic, docosapentaenoic, and docodiene. a model that included a list of fatty acids, such as С14:0, С15:0, С16:1;7, С18:3 n-3, C20:2 n-6, C20:3 n-6, C20:5 n-3, C22:4 n-6, C22:5 n-3, C22:6 n-3, sum of omega-3, omega-3 (EPA + DHA), and saturated / polyunsaturated fatty acids ratio provided auc 0,916 with a specificity of 0,90 and sensitivity of 0,95 in distinguishing patients with stage I-II CRC from healthy individuals.Conclusion. the study of the levels and ratios of fatty acids in erythrocyte membranes and blood serum should be considered a promising trend in the search for biomarkers for the early diagnosis of colorectal cancer.

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In-depth mapping carboxylic acid metabolome reveals the potential biomarkers in colorectal cancer through characteristic fragment ions and metabolic flux

Cited by 22 publications

References 23 publications

In-depth profiling of carboxyl compounds in Chinese Baijiu based on chemical derivatization and ultrahigh-performance liquid chromatography coupled to high-resolution mass spectrometry

In-depth profiling of carboxyl compounds in Chinese Baijiu based on chemical derivatization and ultrahigh-performance liquid chromatography coupled to high-resolution mass spectrometry

A prognostic Risk Score model for oral squamous cell carcinoma constructed by 6 glycolysis-immune-related genes

Fatty acids of erythrocyte membranes and blood serum as biomarkers for early detection of colorectal cancer

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