In depth investigation of collagen non-enzymatic glycation by Raman spectroscopy

Alsamad, Fatima; Brunel, Benjamin; Vuiblet, Vincent; Gillery, Philippe; Jaisson, Stéphane; Piot, Olivier

doi:10.1016/j.saa.2020.119382

Cited by 12 publications

(9 citation statements)

References 42 publications

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“…Assessment of AGE content, including CML, to gauge biomechanical deterioration is common in numerous tissues, and the levels of CML detected are higher than that of the pentosidine ( Verzijl et al, 2002 ). The formation of CML via ribose is confirmed by previous studies ( Chen et al, 2019 ; Ban et al, 2022 ; Alsamad et al, 2021 ). Evidence of a possible relationship between CML and mechanical deterioration of bone is implied by studies that show increased levels of circulating CML that correlate with increased hip fracture risk in older patients independent of bone mineral density, and an increase in circulating CML levels in osteoporotic patients.…”

Section: Introductionsupporting

confidence: 87%

“…Thus, we do not compare the CML levels directly to fAGE (ng/mg collagen) levels. We use a ribosylation method to amplify AGEs in the bone samples, however, it should be noted that ribose favors formation of pentosidine (pentose-derived) over other fAGEs ( Chen et al, 2019 ; Alsamad et al, 2021 ). The fAGEs induced by ribosylation does not allow us to distinguish between non-crosslinking and crosslinking AGEs and quantifies only a small portion of fAGEs, but ribosylation is favored due to its ability to amplify AGEs in fewer days compared to glucose incubation.…”

Section: Discussionmentioning

confidence: 99%

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Accumulation of fluorescent advanced glycation end products and carboxymethyl-lysine in human cortical and trabecular bone

Vaidya¹,

Rezaee²,

Edwards³

et al. 2022

Bone Reports

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Section: Introductionsupporting

confidence: 87%

Section: Discussionmentioning

confidence: 99%

Accumulation of fluorescent advanced glycation end products and carboxymethyl-lysine in human cortical and trabecular bone

Vaidya¹,

Rezaee²,

Edwards³

et al. 2022

Bone Reports

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“…In addition, FT-IR spectroscopy was also shown to be able to identify the subpopulations of extracellular vesicles from different sizes and different cellular origin efficiently and quickly [161]. Recently, Raman spectroscopy was indicated to identify and quantify the molecular modifications of collagen and seems to be an interesting tool to study biological processes [162]. A novel detecting method, single-cell Raman spectroscopy (scRS) coupled with one-dimensional convolutional neural networks (1DCNN) was explored to identify individual marine microorganisms quickly and accurately [163].…”

Section: Developing Technologies For Structure Characterizationmentioning

confidence: 99%

Development of Marine-Derived Compounds for Cancer Therapy

Zuo

Kwok

2021

Marine Drugs

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Cancer has always been a threat to human health with its high morbidity and mortality rates. Traditional therapy, including surgery, chemotherapy and radiotherapy, plays a key role in cancer treatment. However, it is not able to prevent tumor recurrence, drug resistance and treatment side effects, which makes it a very attractive challenge to search for new effective and specific anticancer drugs. Nature is a valuable source of multiple pharmaceuticals, and most of the anticancer drugs are natural products or derived from them. Marine-derived compounds, such as nucleotides, proteins, peptides and amides, have also shed light on cancer therapy, and they are receiving a fast-growing interest due to their bioactive properties. Their mechanisms contain anti-angiogenic, anti-proliferative and anti-metastasis activities; cell cycle arrest; and induction of apoptosis. This review provides an overview on the development of marine-derived compounds with anticancer properties, both their applications and mechanisms, and discovered technologies.

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“…In order to reduce the diversity of the AGEs formed and thus to facilitate the interpretation of the results, glyoxylic acid was chosen as the glycating agent. Treatment of proteins with glyoxylic acid mainly modifies lysine to CML, which is one of the most studied major endogenous and exogenous AGEs [48]. In adulthood, C. elegans can be grown with either living or dead bacteria.…”

Section: Glycation Of Bacteria and Detection Of CML Epitopesmentioning

confidence: 99%

“…We exposed the worms to their classic laboratory food, Escherichia coli bacteria, glycated or non-glycated. For glycated food, bacteria were chemically modified with glyoxylic acid to mainly produce Nε-carboxymethyllysine (CML) [48]. We performed different immunotechniques using an anti-CML antibody for relative quantification of ingested CML and localization of this AGE in the worms' intestine.…”

Section: Introductionmentioning

confidence: 99%

Exposure of Caenorhabditis elegans to Dietary Nε-Carboxymethyllysine Emphasizes Endocytosis as a New Route for Intestinal Absorption of Advanced Glycation End Products

et al. 2021

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The impact of dietary advanced glycation end products (dAGEs) on human health has been discussed in many studies but, to date, no consensual pathophysiological process has been demonstrated. The intestinal absorption pathways which have so far been described for dAGEs, the passive diffusion of free AGE adducts and transport of glycated di-tripeptides by the peptide transporter 1 (PEPT-1), are not compatible with certain pathophysiological processes described. To get new insight into the intestinal absorption pathways and the pathophysiological mechanisms of dAGEs, we initiated an in vivo study with a so-called simple animal model with a complete digestive tract, Caenorhabditis elegans. Dietary bacteria were chemically modified with glyoxylic acid to mainly produce Nε-carboxymethyllysine (CML) and used to feed the worms. We performed different immunotechniques using an anti-CML antibody for the relative quantification of ingested CML and localization of this AGE in the worms’ intestine. The relative expression of genes encoding different biological processes such as response to stresses and intestinal digestion were determined. The physiological development of the worms was verified. All the results were compared with those obtained with the control bacteria. The results revealed a new route for the intestinal absorption of dietary CML (dCML), endocytosis, which could be mediated by scavenger receptors. The exposure of worms to dCML induced a reproductive defect and a transcriptional response reflecting oxidative, carbonyl and protein folding stresses. These data, in particular the demonstration of endocytosis of dCML by enterocytes, open up new perspectives to better characterize the pathophysiological mechanisms of dAGEs.

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In depth investigation of collagen non-enzymatic glycation by Raman spectroscopy

Cited by 12 publications

References 42 publications

Accumulation of fluorescent advanced glycation end products and carboxymethyl-lysine in human cortical and trabecular bone

Accumulation of fluorescent advanced glycation end products and carboxymethyl-lysine in human cortical and trabecular bone

Development of Marine-Derived Compounds for Cancer Therapy

Exposure of Caenorhabditis elegans to Dietary Nε-Carboxymethyllysine Emphasizes Endocytosis as a New Route for Intestinal Absorption of Advanced Glycation End Products

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