In-depth insight into in vivo apoplastic secretome of rice-Magnaporthe oryzae interaction

Kim, Pil Joo; Wang, Yiming; Lee, Kyung Hee; Park, Chul–Seung; Park, Jongsun; Wu, Jingni; Kwon, Soon Jae; Lee, Yong-Hwan; Agrawal, Ganesh Kumar; Rakwal, Randeep; Kim, Sun Tae; Kang, Kyu Young

doi:10.1016/j.jprot.2012.10.029

Cited by 105 publications

(125 citation statements)

References 71 publications

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“…However, 31 of the identified proteins are SecretomeP-positive, suggesting unconventional protein secretion (Nickel and Rabouille, 2009;Pinedo et al, 2012). Similar results were reported from other studies of apoplastic proteins (Fernández et al, 2012;Kim et al, 2013;Wang et al, 2013). Also, immunoblot and MDH enzyme assays indicated that the apoplast extract showed minimal contamination with intracellular components (Fig.…”

Section: -Q)supporting

confidence: 87%

The Thioredoxin GbNRX1 Plays a Crucial Role in Homeostasis of Apoplastic Reactive Oxygen Species in Response to Verticillium dahliae Infection in Cotton

et al. 2016

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Examining the proteins that plants secrete into the apoplast in response to pathogen attack provides crucial information for understanding the molecular mechanisms underlying plant innate immunity. In this study, we analyzed the changes in the root apoplast secretome of the Verticillium wilt-resistant island cotton cv Hai 7124 (Gossypium barbadense) upon infection with Verticillium dahliae. Two-dimensional differential gel electrophoresis and matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometry analysis identified 68 significantly altered spots, corresponding to 49 different proteins. Gene ontology annotation indicated that most of these proteins function in reactive oxygen species (ROS) metabolism and defense response. Of the ROS-related proteins identified, we further characterized a thioredoxin, GbNRX1, which increased in abundance in response to V. dahliae challenge, finding that GbNRX1 functions in apoplastic ROS scavenging after the ROS burst that occurs upon recognition of V. dahliae. Silencing of GbNRX1 resulted in defective dissipation of apoplastic ROS, which led to higher ROS accumulation in protoplasts. As a result, the GbNRX1-silenced plants showed reduced wilt resistance, indicating that the initial defense response in the root apoplast requires the antioxidant activity of GbNRX1. Together, our results demonstrate that apoplastic ROS generation and scavenging occur in tandem in response to pathogen attack; also, the rapid balancing of redox to maintain homeostasis after the ROS burst, which involves GbNRX1, is critical for the apoplastic immune response.

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Section: -Q)supporting

confidence: 87%

The Thioredoxin GbNRX1 Plays a Crucial Role in Homeostasis of Apoplastic Reactive Oxygen Species in Response to Verticillium dahliae Infection in Cotton

et al. 2016

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“…Transcriptome analysis at the early stage of infection has been done using ESTs and SAGE technology for some fungi, e.g. Magnaporthe oryzae [17]. In this work, we identified 11 O. heveae pathogenicityrelated genes through bioinformatic analysis of our genomic data (unpublished).…”

Section: Discussionmentioning

confidence: 99%

Cloning and Expression of the Pathogenicity-Related Genes of Oidium heveae in the Infection Process

Sun¹,

Peng²,

He³

et al. 2015

J Plant Pathol Microbiol

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“…For example, the secretome of rice leaf cells was examined upon infection with the blast fungus Magnaporte oryzae. Proteins involved in antioxidant defense, proteases and pathogenesis-related proteins represented major secreted proteins [32]. Moreover, proteins derived from blast fungus were identified, such as glycosyl hydrolases.…”

Section: Accepted Manuscriptmentioning

confidence: 99%

“…Some of these hydrolases induced fluorescence of such GFP-based cell death marker, similarly to microbial effectors. This suggests that glycosyl hydrolases may act as Magnaporte oryzae effectors [32]. The same approach was used also in rice infected by Xanthomonas campestris [33], identifying regulator of pathogenicity factor protein and two hypothetical proteins as putative effectors.…”

Section: Accepted Manuscriptmentioning

confidence: 99%

Integrating cell biology and proteomic approaches in plants

Takáč

Šamajová

Šamaj

2017

Journal of Proteomics

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In-depth insight into in vivo apoplastic secretome of rice-Magnaporthe oryzae interaction

Cited by 105 publications

References 71 publications

The Thioredoxin GbNRX1 Plays a Crucial Role in Homeostasis of Apoplastic Reactive Oxygen Species in Response to Verticillium dahliae Infection in Cotton

The Thioredoxin GbNRX1 Plays a Crucial Role in Homeostasis of Apoplastic Reactive Oxygen Species in Response to Verticillium dahliae Infection in Cotton

Cloning and Expression of the Pathogenicity-Related Genes of Oidium heveae in the Infection Process

Integrating cell biology and proteomic approaches in plants

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