In‐depth examination of hyperproliferative healing in two breeds of <i>Sus scrofa domesticus</i> commonly used for research

Funkhouser, Colton; Kirkpatrick, Liam D.; Smith, Robert; Moffatt, Lauren T.; Shupp, Jeffrey W.; Carney, Bonnie C

doi:10.1002/ame2.12188

Cited by 2 publications

(2 citation statements)

References 37 publications

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“…In addition, the melanin contained within HTS for patients with skin of color may mask erythema that is otherwise visible in fair-skinned patients. However, we have shown that hyper- and hypo-pigmented HTS in red Duroc pigs contain significantly increased vasculature compared to normal skin even if they do not appear to the eye as erythematous 29 . Therefore, it is likely that all patients could benefit from pharmacologic treatments that target endothelial cells and vasculature that may not be possible with light-based treatments.…”

Section: Introductionmentioning

confidence: 74%

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Angiogenic gene characterization and vessel permeability of dermal microvascular endothelial cells isolated from burn hypertrophic scar

Molina

Hartmann

Oliver

et al. 2022

Sci Rep

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Hypertrophic scar (HTS) formation is a common challenge for patients after burn injury. Dermal microvascular endothelial cells (DMVECs) are an understudied cell type in HTS. An increase in angiogenesis and microvessel density can be observed in HTS. Endothelial dysfunction may play a role in scar development. This study aims to generate a functional and expression profile of HTS DMVECs. We hypothesize that transcript and protein-level responses in HTS DMVECs differ from those in normal skin (NS). HTSs were created in red Duroc pigs. DMVECs were isolated using magnetic-activated cell sorting with ulex europaeus agglutinin 1 (UEA-1) lectin. Separate transwell inserts were used to form monolayers of HTS DMVECs and NS DMVECs. Cell injury was induced and permeability was assessed. Gene expression in HTS DMVECS versus NS DMVECs was measured. Select differentially expressed genes were further investigated. HTS had an increased area density of dermal microvasculature compared to NS. HTS DMVECs were 17.59% less permeable than normal DMVECs (p < 0.05). After injury, NS DMVECs were 28.4% and HTS DMVECs were 18.8% more permeable than uninjured controls (28.4 ± 4.8 vs 18.8 ± 2.8; p = 0.11). PCR array identified 31 differentially expressed genes between HTS and NS DMVECs, of which 10 were upregulated and 21 were downregulated. qRT-PCR and ELISA studies were in accordance with the array. DMVECs expressed a mixed profile of factors that can contribute to and inhibit scar formation. HTS DMVECs have both a discordant response to cellular insults and baseline differences in function, supporting their proposed role in scar pathology. Further investigation of DMVECs is warranted to elucidate their contribution to HTS pathogenesis.

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Section: Introductionmentioning

confidence: 74%

“…1 E). We have previously reported the presence of hypervascularity in HTS with the use of alpha-smooth muscle actin staining 29 . HTSs from the current study appear to be hypervascular compared to normal skin based on representative sections stained using Verhoeff-Van Gieson stain to observe blood vessels (Fig.…”

Section: Resultsmentioning

confidence: 99%

Angiogenic gene characterization and vessel permeability of dermal microvascular endothelial cells isolated from burn hypertrophic scar

Molina

Hartmann

Oliver

et al. 2022

Sci Rep

Self Cite

View full text Add to dashboard Cite

show abstract

The investigation of batch-to-batch variabilities in the composition of isolates from fish and mammalian species using different protocols

Rýglová

Braun

Suchý

et al. 2023

Food Research International

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In‐depth examination of hyperproliferative healing in two breeds of Sus scrofa domesticus commonly used for research

Abstract: This is an open access article under the terms of the Creat ive Commo ns Attri butio n-NonCo mmerc ial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

Cited by 2 publications

References 37 publications

Angiogenic gene characterization and vessel permeability of dermal microvascular endothelial cells isolated from burn hypertrophic scar

Angiogenic gene characterization and vessel permeability of dermal microvascular endothelial cells isolated from burn hypertrophic scar

The investigation of batch-to-batch variabilities in the composition of isolates from fish and mammalian species using different protocols

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