In-depth comparative transcriptome analysis of intestines of red swamp crayfish, Procambarus clarkii, infected with WSSV

Abstract

Crayfish has become one of the most important farmed aquatic species in China due to its excellent disease resistance against bacteria and viruses. However, the antiviral mechanism of crayfish is still not very clear. In the present study, many high-quality sequence reads from crayfish intestine were obtained using Illumina-based transcriptome sequencing. For the normal group (GN), 44,600,142 high-quality clean reads were randomly assembled to produce 125,394 contigs. For the WSSV-challenged group (GW), 47,790,746 high-quality clean reads were randomly assembled to produce 148,983 contigs. After GO annotation, 39,482 unigenes were annotated into three ontologies: biological processes, cellular components, and molecular functions. In addition, 15,959 unigenes were mapped to 25 different COG categories. Moreover, 7,000 DEGs were screened out after a comparative analysis between the GN and GW samples, which were mapped into 250 KEGG pathways. Among these pathways, 36 were obviously changed (P-values < 0.05) and 28 pathways were extremely significantly changed (P-values < 0.01). Finally, five key DEGs involved in the JAK-STAT signaling pathway were chosen for qRT-PCR. The results showed that these five DEGs were obviously up-regulated at 36 h post WSSV infection in crayfish intestine. These results provide new insight into crayfish antiviral immunity mechanisms.

Insecticidal Activity of Melaleuca alternifolia Essential Oil and RNA-Seq Analysis of Sitophilus zeamais Transcriptome in Response to Oil Fumigation

“…Determination of the activity of these enzymes in insects after insecticide applications has been widely performed to better understand the insecticidal mechanism of xenobiotic compounds [19]. On the other hand, transcriptional regulation of gene expression in insects has been found to play an important role in insect response to various stressors [20, 21]. However, up to now, there is no any report about a global gene expression profile of pest insects in response to plant essential oils.…”

Identification and characterization of intestine microRNAs and targets in red swamp crayfish, Procambarus clarkii infected with white spot syndrome virus

“…P . clarkii (approximately 15–20 g) were purchased from a commercial aquaculture market in Hangzhou, Zhejiang Province, China and were cultivated in water tanks at 26–28°C for 10 days to adapt to the surviving environment [ 1 ]. All crayfish were fed twice daily with artificial food throughout the entire experiment.…”

“…Procambarus clarkii is frequently used as a model organism to study the molecular mechanisms of innate immunity in invertebrates [ 1 ]. P .…”

Comparative transcriptome analysis reveals three potential antiviral signaling pathways in lymph organ tissue of the red swamp crayfish, Procambarus clarkii

“…The expressed sequences produced using NGS technology are usually ten-to one-hundred-fold greater in number than those identified by traditional Sanger sequencing technologies (Christie et al, 2015). In a previous study, the Janus kinase/signal transducers and activators of transcription (JAK-STAT) signaling pathway was found to play an important role in crayfish intestinal antiviral immunity (Du et al, 2016). In the present study, we sequenced the lymph organ transcriptomes of normal and WSSV-challenged crayfish in order to generate expression profiles and identify differentially expressed genes (DEGs) between normal crayfish's lymph organs and WSSV-challenged crayfish's lymph organs.…”