In chemico skin sensitization risk assessment of botanical ingredients

Abstract

Skin sensitization risk assessment of botanical ingredients is necessary for consumers' protection and occupational hazard identification. There are currently very few available alternative methods that can assist in the evaluation of complex mixtures. Chemical methods can provide essential information in a timely manner and thus help to reduce the need for in vivo testing, and they can complement and facilitate targeted in vitro assays. In the present work, the applicability of the high-throughput screening with dansyl cysteamine (DCYA) method for the systematic evaluation of skin sensitization of complex botanicals was explored. Botanical ingredients of four unrelated plant species were obtained and tested with the high-throughput fluorescence method at three concentrations. To illustrate the minimal matrix effects of the tested extracts on the developed method, the least DCYA-reactive extract (Rosa canina) was spiked with known sensitizers at different concentrations. The data obtained from the four plant extracts and the spiking experiments with known sensitizers, suggest that the high-throughput screening-DCYA method can be successfully applied for estimating the skin sensitization potential of complex botanical matrices. This is the first report of an attempt to develop a versatile in chemico method for the rapid detection of reactive skin sensitizers in complex botanical extracts, which could complement the battery of existing validated, non-animal methods.

Identification of Potential Skin Sensitizers in Myrrh

“…The myrrh extract and fractions were tested at three concentrations (5, 10, and 20 mg/mL) as previously described [13]. Negative and positive controls (NC and PC) were included to estimate the minimal and maximal fluorescence response.…”

“…In chemico methods can represent a powerful tool at pre-screening stages by providing information about the likelihood of a molecule to covalently bind to skin proteins [12]. An in chemico approach for the rapid investigation of the presence of potential skin sensitizers in complex botanical mixtures was recently described [13]. The high-throughput screening method using dansylated cysteamine (HTS-DCYA™) enables the stable derivatization of thio-reactive chemical sensitizers as fluorescent dansylated adducts, which can be quantified through a fluorescence assay in a 96-well format.…”

Phytochemical investigation of Magnolia grandiflora green seed cones: Analytical and phytoceutical studies

“…Magnolias (belong to the family Magnoliaceae ) are known worldwide for their beautiful flowers with intense fragrance. Many species of the genus Magnolia are grown around the world, and out of all these different species, Magnolia grandiflora is the most attractive one because of its large size, gorgeous color, and fragrance (Avonto, Chittiboyina, Sadrieh, Vukmanovic, & Khan, ; Ding et al, ; Lata et al, ). This article deals with the phytochemical investigation of the diethyl ether and ethanol extracts derived from the Magnolia grandiflora green seed cones.…”