In-cell processing enables rapid and in-depth proteome analysis of low-inputCaenorhabditis elegans
Malek Elsayyid,
Jessica E. Tanis,
Yanbao Yu
Abstract:Caenorhabditis elegans is a widely used genetic model organism, however, the worm cuticle complicates extraction of intracellular proteins, a prerequisite for typical bottom-up proteomics. Conventional physical disruption procedures are not only time-consuming, but can also cause significant sample loss, making it difficult to perform proteomics with low-input samples. Here, for the first time, we present an on-filter in-cell (OFIC) processing approach, which can digest C. elegans proteins directly in the cell… Show more
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