IMXQB-80: A Quillaja brasiliensis saponin-based nanoadjuvant enhances Zika virus specific immune responses in mice

Cibulski, Samuel Paulo; Teixeira, Thaís Fumaco; Varela, Ana Paula Muterle; Lima, Matheus Fabião de; Casanova, Gabriela; Nascimento, Yuri Mangueira do; Tavares, Josean Fechine; Silva, Marcelo Sobral da; Sesterheim, Patrícia; Souza, Diogo Onofre Gomes de; Roehe, Paulo Michel; Silveira, Fernando

doi:10.1016/j.vaccine.2020.12.004

Cited by 21 publications

(20 citation statements)

References 37 publications

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“…Recently, a phytochemical study conducted to identify the bioactive constituents of H. hirsuta has shown that the aerial part of this plant is rich in phenolic compounds ( Figure 7 a,b) [ 171 ]. According to the literature, these compounds are well known for various pharmacological effects [ 175 , 176 , 177 , 178 , 179 ]. Therefore, the antilithiasic activity of H. hirsuta may be due to the presence of these compounds.…”

Section: Resultsmentioning

confidence: 99%

Inventory of Medicinal Plants Used Traditionally to Manage Kidney Diseases in North-Eastern Morocco: Ethnobotanical Fieldwork and Pharmacological Evidence

Bencheikh

Elbouzidi

Kharchoufa

et al. 2021

Plants

109

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Kidney disease is one of the most common health problems and kidney failure can be fatal. It is one of the health disorders associated with extreme pain and discomfort in patients. In developing countries, such as Morocco where socioeconomic and sanitary conditions are precarious, medicinal plants are considered the primary source of medication. In the present work an ethnobotanical survey was conducted in a remote area of North-Eastern Morocco and we focused on (1) establishing a record of medicinal plants used traditionally by local people to treat kidney diseases and (2) correlate the obtained ethnomedical use with well-studied pharmacological evidence. From February 2018 to January2020, information was gathered from 488 informants using semi-structured questionnaires. The data were analyzed using three quantitative indices: The use value (UV), family use value (FUV), and informant consensus factor (ICF). A total of 121 plant species belonging to 57 botanical families were identified to treat kidney diseases. The families most represented were Asteraceae (14 species), followed by Lamiaceae (12 species) and Apiaceae (10 species). The most commonly used plant parts were leaves, followed by the whole plant and they were most commonly prepared by decoction and infusion. The highest value of the (UV) index was attributed to Herniaria hirsuta L. (UV = 0.16), and the highest family use value (FUV) was assigned to Caryophyllaceae with (FUV = 0.163). Regarding the informant consensus factor (ICF), this index’s highest values were recorded for kidney stones (ICF = 0.72). The use of 45% of the selected plants were validated based on literature review. This study helped document and preserve crucial traditional plant knowledge of 121 plant species used to treat kidney problems that can be used in the search for new biologically active compounds through more upcoming pharmacological studies.

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Section: Resultsmentioning

confidence: 99%

Inventory of Medicinal Plants Used Traditionally to Manage Kidney Diseases in North-Eastern Morocco: Ethnobotanical Fieldwork and Pharmacological Evidence

Bencheikh

Elbouzidi

Kharchoufa

et al. 2021

Plants

109

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“…In addition, physicochemical characterization methods were described and validated in order to analyze and quantify the QB-90 saponins fractions obtained [19]. In particular, using LC-MS [46,47], MALDI-TOF [18], and DI-ESI-TOF [17] for the characterization of Q. brasiliensis saponins have been previously described as valuable, fast, and cheap tools to analyze saponins fractions.…”

Section: Concluding Remarks and Perspectivesmentioning

confidence: 99%

“…Immune-stimulating complexes (ISCOMs) are cage-like nanoparticles of approximately 40 nm, obtained combining Q. saponaria saponins, lipids, and protein antigens [12]. Our group has been studying the adjuvant capacity of saponins obtained from the leaves of Q. brasiliensis [13][14][15][16][17][18][19], a native tree from South America [20]. Previous reports have shown remarkable similarities between QB-90 fraction and QuilA saponins, both in their chemical structure and from an immunological properties perspective when used with herpesvirus [21] and poliovirus [14] antigens in mouse models.…”

Section: Introductionmentioning

confidence: 99%

ISCOM-like Nanoparticles Formulated with Quillaja brasiliensis Saponins Are Promising Adjuvants for Seasonal Influenza Vaccines

et al. 2021

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Vaccination is the most effective public health intervention to prevent influenza infections, which are responsible for an important burden of respiratory illnesses and deaths each year. Currently, licensed influenza vaccines are mostly split inactivated, although in order to achieve higher efficacy rates, some influenza vaccines contain adjuvants. Although split-inactivated vaccines induce mostly humoral responses, tailoring mucosal and cellular immune responses is crucial for preventing influenza infections. Quillaja brasiliensis saponin-based adjuvants, including ISCOM-like nanoparticles formulated with the QB-90 saponin fraction (IQB90), have been studied in preclinical models for more than a decade and have been demonstrated to induce strong humoral and cellular immune responses towards several viral antigens. Herein, we demonstrate that a split-inactivated IQB90 adjuvanted influenza vaccine triggered a protective immune response, stronger than that induced by a commercial unadjuvanted vaccine, when applied either by the subcutaneous or the intranasal route. Moreover, we reveal that this novel adjuvant confers up to a ten-fold dose-sparing effect, which could be crucial for pandemic preparedness. Last but not least, we assessed the role of caspase-1/11 in the generation of the immune response triggered by the IQB90 adjuvanted influenza vaccine in a mouse model and found that the cellular-mediated immune response triggered by the IQB90-Flu relies, at least in part, on a mechanism involving the casp-1/11 pathway but not the humoral response elicited by this formulation.

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“…This procedure resulted in the nanoadjuvant derived from QB-80, which was named IQB80. In order to check the toxicity of the nanoadjuvant, hemolysis tests were performed, as previously described (17,18,24), and none of the concentrations tested (ranging from 100 to 10 µg) have been shown to cause membrane damage (data not shown). The experimental vaccine formulations were prepared under aseptic conditions, filtered through 0.22 µm filters and kept at 4 • C until use.…”

Section: Nanoadjuvant Vaccine Preparationmentioning

confidence: 99%

Zika Virus Envelope Domain III Recombinant Protein Delivered With Saponin-Based Nanoadjuvant From Quillaja brasiliensis Enhances Anti-Zika Immune Responses, Including Neutralizing Antibodies and Splenocyte Proliferation

et al. 2021

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Nanoadjuvants that combine immunostimulatory properties and delivery systems reportedly bestow major improvements on the efficacy of recombinant, protein-based vaccines. Among these, self-assembled micellar formulations named ISCOMs (immune stimulating complexes) show a great ability to trigger powerful immunological responses against infectious pathogens. Here, a nanoadjuvant preparation, based on saponins from Quillaja brasiliensis, was evaluated together with an experimental Zika virus (ZIKV) vaccine (IQB80-zEDIII) and compared to an equivalent vaccine with alum as the standard adjuvant. The preparations were administered to mice in two doses (on days zero and 14) and immune responses were evaluated on day 28 post-priming. Serum levels of anti-Zika virus IgG, IgG1, IgG2b, IgG2c, IgG3 were significantly increased by the nanoadjuvant vaccine, compared to the mice that received the alum-adjuvanted vaccine or the unadjuvanted vaccine. In addition, a robust production of neutralizing antibodies and in vitro splenocyte proliferative responses were observed in mice immunized with IQB80-zEDIII nanoformulated vaccine. Therefore, the IQB80-zEDIII recombinant preparation seems to be a suitable candidate vaccine for ZIKV. Overall, this study identified saponin-based delivery systems as an adequate adjuvant for recombinant ZIKV vaccines and has important implications for recombinant protein-based vaccine formulations against other flaviviruses and possibly enveloped viruses.

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IMXQB-80: A Quillaja brasiliensis saponin-based nanoadjuvant enhances Zika virus specific immune responses in mice

Cited by 21 publications

References 37 publications

Inventory of Medicinal Plants Used Traditionally to Manage Kidney Diseases in North-Eastern Morocco: Ethnobotanical Fieldwork and Pharmacological Evidence

Inventory of Medicinal Plants Used Traditionally to Manage Kidney Diseases in North-Eastern Morocco: Ethnobotanical Fieldwork and Pharmacological Evidence

ISCOM-like Nanoparticles Formulated with Quillaja brasiliensis Saponins Are Promising Adjuvants for Seasonal Influenza Vaccines

Zika Virus Envelope Domain III Recombinant Protein Delivered With Saponin-Based Nanoadjuvant From Quillaja brasiliensis Enhances Anti-Zika Immune Responses, Including Neutralizing Antibodies and Splenocyte Proliferation

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