Like other halophytic chenopods, sugar beet (Beta vulgaris L.) can accumulate high betaine levels in shoots and roots. N,N,N-trimethylglycine impedes sucrose crystallization and so lowers beet quality. among sugar beets and their relatives, about the role of betaine in the salt-tolerance of these plants, or about the synthesis and metabolism of betaine in the chenopods as a group (for reviews, see 7, 22). Betaine accumulation, whether constitutive or saltinduced, may be a specific adaptation for salt-tolerance in wild and cultivated members of the Chenopodiaceae, including Beta spp. (for reviews, see 21, 22). Histochemical, biochemical, and physiological evidence for such chenopods indicates that betaine is an inert and nontoxic cytoplasmic osmoticum that helps to maintain osmotic equilibrium between the cytoplasm and the vacuole as the vacuolar solute potential is lowered by salt accumulation (6,15,20,21). High betaine levels in sugar beet roots are undesirable to the beet sugar industry because betaine interferes with sucrose crystallization from the juice (e.g. 23). The molar concentration of betaine in juice can reach 5% to 10% that of sucrose (e.g. 17). High levels of betaine in sugar beet roots could conceivably reduce sugar yields in a second way: the synthesis of 1 mol of betaine requires about the same energy input as that of 1 mol of sucrose (11); therefore, photosynthate diverted to betaine represents an appreciable cost in energy and photosynthate that is neither available for storage as sucrose, nor for plant processes that contribute indirectly to economic yield. If useful genetic variability for root betaine level exists within the primary gene pool of B. vulgaris, in principle low-betaine types could be developed by breeding. Whether lowering betaine levels by genetic means would be worthwhile in practice hinges on several sets of considerations, including: (a) ecological and physiological considerations, which suggest that salt-tolerance (or more generally, ionic regulation) might be adversely affected; (b) metabolic considerations, which indicate that plant performance might suffer if betaine has some metabolic function and is not merely an inert osmoticum; (c) technological and bioenergetic considerations, which imply that sucrose yields would increase.In this paper, we present information that bears on considerations (a) and (b) in breeding for low-betaine sugar beet types.Very little is known about genetic variability for betaine3 level