Impurities identification and quantification for calcitonin salmon by liquid chromatography-high resolution mass spectrometry

Wu, Peize; Li, Ming; Kan, Yanmei; Wu, Xue Shen; Li, Hongmei

doi:10.1016/j.jpba.2020.113271

Cited by 4 publications

(4 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A mixture solution of synthetic A1 peptide and A2 peptide standards (15 μg/mL) was used to optimize the liquid chromatography gradient. A core–shell LC column, which was previously found to be suitable for the separation of peptides [ 26 , 27 ], was chosen for the separation of β-CN digestion products. A1 peptide and A2 peptide can be completely separated, as shown in Figure 2 .…”

Section: Resultsmentioning

confidence: 99%

Determination of A1 and A2 β-Casein in Milk Using Characteristic Thermolytic Peptides via Liquid Chromatography-Mass Spectrometry

Liu

Pan²,

et al. 2023

Molecules

Self Cite

View full text Add to dashboard Cite

β-casein, a protein in milk and dairy products, has two main variant forms termed as A1 and A2. A1 β-casein may have adverse effects on humans. The fact that there is only one amino acid variation at the 67th position between A1 and A2 β-casein makes it difficult to distinguish between them. In this study, a novel method using characteristic thermolytic peptides is developed for the determination of A1 and A2 β-casein in milk. Firstly, caseins extracted from milk samples are thermolytic digested at 60 °C without any denaturing reagents required for unfolding proteins, which simplifies the sample pretreatment procedure. The characteristic thermolytic peptides (i.e., fragments 66–76 and 59–76 for A1 and A2 β-casein, respectively) selected to specifically distinguish A1 and A2 β-casein only have eleven or eighteen amino acid moieties. Compared with tryptic characteristic peptides with a length of 49 amino acid moieties, these shorter thermolytic characteristic peptides are more suitable for LC-MS analysis. This novel method, with the advantages of high specificity, high sensitivity, and high efficiency, was successfully applied for the analysis of six milk samples collected from a local supermarket. After further investigation, it is found that this method would contribute to the development of A2 dairy products for a company and the quality inspection of A2 dairy products for a government.

show abstract

Section: Resultsmentioning

confidence: 99%

Determination of A1 and A2 β-Casein in Milk Using Characteristic Thermolytic Peptides via Liquid Chromatography-Mass Spectrometry

Liu

Pan²,

et al. 2023

Molecules

Self Cite

View full text Add to dashboard Cite

show abstract

“…This work shows the importance of using new technologies on legacy drug projects as the advances in HRMS allow a better understanding of drugs and their impurities that was not available before. Being able to detect the low‐level impurities helps ensure patient safety 70 . Similarly, this group performed the same type of experiments for arginine vasopressin, which is a cyclic peptide drug to treat diabetes insipidus and esophageal varices bleeding 71 …”

Section: Post‐approval Analyses and Hrms Quantificationmentioning

confidence: 99%

“…Being able to detect the lowlevel impurities helps ensure patient safety. 70 Similarly, this group performed the same type of experiments for arginine vasopressin, which is a cyclic peptide drug to treat diabetes insipidus and esophageal varices bleeding. 71 Brinkman et al used GC-TOF-MS and UHPLC-Q-TOF-MS to compare the excreted levels of asthma medication in exhaled breath and urine samples, respectively, in severe asthma patients.…”

Section: Quality Control and Surveillancementioning

confidence: 99%

See 1 more Smart Citation

Advances in high‐resolution mass spectrometry applied to pharmaceuticals in 2020: A whole new age of information

Géhin

Holman

2021

Analytical Science Advances

View full text Add to dashboard Cite

Continuous improvements in mass spectrometry (MS) have resulted in the widespread availability and adoption of high‐resolution mass spectrometry (HRMS) across laboratories worldwide. The capabilities and the associated advantages of HRMS make it an invaluable analytical tool for analyte characterization, screening, and quantification methodologies for a wide scope of applications across pharmaceutical development. These applications include drug discovery, product characterizations of both small molecules and novel drug modalities, in vitro and in vivo metabolism studies, post‐approval quality control, and pharmacovigilance. This review gives an overview of the current capabilities of HRMS and its pharmaceutical applications in 2020, and provides a perspective on the future of HRMS within the pharmaceutical industry.

show abstract

Identification and determination of structurally related peptide impurities in thymalfasin by liquid chromatography-high-resolution mass spectrometry

Cheng

Kan

et al. 2022

Anal Bioanal Chem

View full text Add to dashboard Cite

Impurities identification and quantification for calcitonin salmon by liquid chromatography-high resolution mass spectrometry

Cited by 4 publications

References 12 publications

Determination of A1 and A2 β-Casein in Milk Using Characteristic Thermolytic Peptides via Liquid Chromatography-Mass Spectrometry

Determination of A1 and A2 β-Casein in Milk Using Characteristic Thermolytic Peptides via Liquid Chromatography-Mass Spectrometry

Advances in high‐resolution mass spectrometry applied to pharmaceuticals in 2020: A whole new age of information

Identification and determination of structurally related peptide impurities in thymalfasin by liquid chromatography-high-resolution mass spectrometry

Contact Info

Product

Resources

About