Improving wood properties for wood utilization through multi-omics integration in lignin biosynthesis

Wang, Jack; Matthews, Megan L.; Williams, Cranos M.; Shi, Rui; Yang, Chenmin; Tunlaya-Anukit, Sermsawat; Chen, Hsi Chuan; Li, Quanzi; Liu, Jie; Lin, Chien Yuan; Naik, Punith P.; Sun, Ying-Hsuan; Loziuk, Philip L.; Yeh, Ting Feng; Kim, Hoon; Gjersing, Erica; Shollenberger, Todd; Shuford, Christopher M.; Song, Jina; Miller, Zachary D.; Huang, Yung Yun; Edmunds, Charles W.; Liu, Baoguang; Sun, Yi; Lin, Ying‐Chung Jimmy; Li, Wei; Chen, Hao; Peszlen, Ilona; Ducoste, Joel J.; Ralph, John; Chang, Hyeyoon; Muddiman, David C.; Davis, Mark F.; Smith, C.A.; Isik, Fikret; Sederoff, Ronald R.; Chiang, Vincent L.

doi:10.1038/s41467-018-03863-z

Cited by 184 publications

(252 citation statements)

References 49 publications

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“…All enzymes and their corresponding genes responsible for the biosynthesis of monolignols have been extensively characterized and their roles in the synthesis and structure of lignin have been genetically investigated using knockout and/or knockdown studies (reviewed by Chapple, 2010 andMottiar et al, 2016). In Arabidopsis and poplar, all monolignol biosynthetic genes have been characterized (Raes et al, 2003;Shi et al, 2010) and their corresponding mutants/downregulated plants and associated phenotypes have been comprehensively analyzed (Vanholme et al, 2012;Wang et al, 2018). A severe reduction in lignin synthesis often leads to a retardation in plant growth and, in some cases, this growth defect could be partially alleviated by altering signaling pathways.…”

Section: Lignin Biosynthesismentioning

confidence: 99%

Secondary cell wall biosynthesis

2018

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Contents Summary1703I.Introduction1703II.Cellulose biosynthesis1705III.Xylan biosynthesis1709IV.Glucomannan biosynthesis1713V.Lignin biosynthesis1714VI.Concluding remarks1717Acknowledgements1717References1717 Summary Secondary walls are synthesized in specialized cells, such as tracheary elements and fibers, and their remarkable strength and rigidity provide strong mechanical support to the cells and the plant body. The main components of secondary walls are cellulose, xylan, glucomannan and lignin. Biochemical, molecular and genetic studies have led to the discovery of most of the genes involved in the biosynthesis of secondary wall components. Cellulose is synthesized by cellulose synthase complexes in the plasma membrane and the recent success of in vitro synthesis of cellulose microfibrils by a single recombinant cellulose synthase isoform reconstituted into proteoliposomes opens new doors to further investigate the structure and functions of cellulose synthase complexes. Most genes involved in the glycosyl backbone synthesis, glycosyl substitutions and acetylation of xylan and glucomannan have been genetically characterized and the biochemical properties of some of their encoded enzymes have been investigated. The genes and their encoded enzymes participating in monolignol biosynthesis and modification have been extensively studied both genetically and biochemically. A full understanding of how secondary wall components are synthesized will ultimately enable us to produce plants with custom‐designed secondary wall composition tailored to diverse applications.

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Section: Lignin Biosynthesismentioning

confidence: 99%

Secondary cell wall biosynthesis

2018

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“…Very recently, Wang et al . () developed a multi‐omics integrative analysis of lignin biosynthesis in poplar to quantify the effects of the expression of monolignol biosynthetic pathway genes on wood properties.…”

Section: Introductionmentioning

confidence: 99%

A systems biology view of wood formation in Eucalyptus grandis trees submitted to different potassium and water regimes

et al. 2019

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Summary Wood production in fast‐growing Eucalyptus grandis trees is highly dependent on both potassium (K) fertilization and water availability but the molecular processes underlying wood formation in response to the combined effects of these two limiting factors remain unknown. E. grandis trees were submitted to four combinations of K‐fertilization and water supply. Weighted gene co‐expression network analysis and MixOmics‐based co‐regulation networks were used to integrate xylem transcriptome, metabolome and complex wood traits. Functional characterization of a candidate gene was performed in transgenic E. grandis hairy roots. This integrated network‐based approach enabled us to identify meaningful biological processes and regulators impacted by K‐fertilization and/or water limitation. It revealed that modules of co‐regulated genes and metabolites strongly correlated to wood complex traits are in the heart of a complex trade‐off between biomass production and stress responses. Nested in these modules, potential new cell‐wall regulators were identified, as further confirmed by the functional characterization of EgMYB137. These findings provide new insights into the regulatory mechanisms of wood formation under stressful conditions, pointing out both known and new regulators co‐opted by K‐fertilization and/or water limitation that may potentially promote adaptive wood traits.

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“…In poplar (Populus spp.) and Arabidopsis (Arabidopsis thaliana), manipulation of lignin composition by altering expression of genes in the phenylpropanoid pathway has provided materials with various ratios of syringyl (S) to guaiacyl G, monolignols (Coleman et al, 2008;Huntley et al, 2003;Min et al, 2013;Smith et al, 2015;Wang et al, 2018;Weng and Chapple, 2010). Characterization of cell structure in woody material by electron microscopy showed that cell separation occurred in genetic variants with high S-lignin after removal of xylan, a (1?4)-blinked polymer of xylose, using maleic acid catalysis (Ciesielski et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Rhamnogalacturonan‐I is a determinant of cell–cell adhesion in poplar wood

Yang

Benatti

Karve

et al. 2019

Plant Biotechnology Journal

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Summary The molecular basis of cell–cell adhesion in woody tissues is not known. Xylem cells in wood particles of hybrid poplar (Populus tremula × P. alba cv. INRA 717‐1B4) were separated by oxidation of lignin with acidic sodium chlorite when combined with extraction of xylan and rhamnogalacturonan‐I (RG‐I) using either dilute alkali or a combination of xylanase and RG‐lyase. Acidic chlorite followed by dilute alkali treatment enables cell–cell separation by removing material from the compound middle lamellae between the primary walls. Although lignin is known to contribute to adhesion between wood cells, we found that removing lignin is a necessary but not sufficient condition to effect complete cell–cell separation in poplar lines with various ratios of syringyl:guaiacyl lignin. Transgenic poplar lines expressing an Arabidopsis thaliana gene encoding an RG‐lyase (AtRGIL6) showed enhanced cell–cell separation, increased accessibility of cellulose and xylan to hydrolytic enzyme activities, and increased fragmentation of intact wood particles into small cell clusters and single cells under mechanical stress. Our results indicate a novel function for RG‐I, and also for xylan, as determinants of cell–cell adhesion in poplar wood cell walls. Genetic control of RG‐I content provides a new strategy to increase catalyst accessibility and saccharification yields from woody biomass for biofuels and industrial chemicals.

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Improving wood properties for wood utilization through multi-omics integration in lignin biosynthesis

Cited by 184 publications

References 49 publications

Secondary cell wall biosynthesis

Secondary cell wall biosynthesis

A systems biology view of wood formation in Eucalyptus grandis trees submitted to different potassium and water regimes

Rhamnogalacturonan‐I is a determinant of cell–cell adhesion in poplar wood

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