Improving the quality of cryopreserved goat semen with a commercial bull extender supplemented with resveratrol

Lv, Chunrong; Allai, Larbi; Wu, Guoquan; Hong, Qionghua; Quan, Guobo

doi:10.1016/j.anireprosci.2019.106127

Cited by 37 publications

(30 citation statements)

References 67 publications

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“…Substantial improvements in preserved (liquid or freezing storage) semen quality traits and sperm fertilization [ 130 , 131 , 133 , 134 ], oocyte development and competence, and embryo development [ 15 , 16 , 139 , 140 ] have been reported. The other evidence that could be observed from in vitro studies is that supplementation of polyphenolic compounds during gametes or embryo in vitro manipulation might continue to in vivo animals.…”

Section: Polyphenols and In Vitro Assisted Reproduction Techniquesmentioning

confidence: 99%

Polyphenols in Farm Animals: Source of Reproductive Gain or Waste?

2020

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Reproduction is a complex process that is substantially affected by environmental cues, specifically feed/diet and its components. Farm animals as herbivorous animals are exposed to a large amount of polyphenols present in their natural feeding system, in alternative feed resources (shrubs, trees, and agro-industrial byproducts), and in polyphenol-enriched additives. Such exposure has increased because of the well-known antioxidant properties of polyphenols. However, to date, the argumentation around the impacts of polyphenols on reproductive events is debatable. Accordingly, the intensive inclusion of polyphenols in the diets of breeding animals and in media for assisted reproductive techniques needs further investigation, avoiding any source of reproductive waste and achieving maximum benefits. This review illustrates recent findings connecting dietary polyphenols consumption from different sources (conventional and unconventional feeds) with the reproductive performance of farm animals, underpinned by the findings of in vitro studies in this field. This update will help in formulating proper diets, optimizing the introduction of new plant species, and feed additives for improving reproductive function, avoiding possible reproductive wastes and maximizing possible benefits.

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Section: Polyphenols and In Vitro Assisted Reproduction Techniquesmentioning

confidence: 99%

Polyphenols in Farm Animals: Source of Reproductive Gain or Waste?

2020

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“…Antioxidants provide a cryoprotective effect improving post-thawing sperm motility, viability, membrane and DNA integrity in bovine, boar, buffalo, stallion and deer spermatozoa [ 13 , 14 , 15 , 16 , 17 ]. In goats, the supplementation of semen extender with several antioxidants, such as quercetin, vitamin C and resveratrol, improved frozen-thawed sperm quality [ 18 , 19 , 20 ]. Recent in vitro studies carried out on carotenoids, organic pigments produced by plants and algae, reported a protective effect of these substances on DNA fragmentation and lipid peroxidation in human [ 21 ] and bovine spermatozoa [ 22 ].…”

Section: Introductionmentioning

confidence: 99%

Crocin Improves the Quality of Cryopreserved Goat Semen in Different Breeds

Longobardi

Zullo

Cotticelli

et al. 2020

Animals

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The effect of crocin in the semen extender before cryopreservation was evaluated on sperm parameters of 20 bucks of five different breeds: Garganica (GA), Jonica (JO), Maltese (MA), Mediterranean Red (MR) and Saanen (SA). Semen samples were centrifuged, to remove seminal plasma, divided in two aliquots and diluted with Tris-egg-yolk-based extender, containing 0 (control group) and 1 mM crocin. Crocin concentration was established after a preliminary dose trial. On fresh and frozen-thawed sperm, motility, viability, morphology, membrane integrity, DNA fragmentation and ROS levels were evaluated. The freezing process led to a decrease (p < 0.05) in all the sperm parameters recorded, confirming the deleterious effect of cryopreservation on goat semen. The most interesting result regarding the inclusion of crocin in the extender before cryopreservation was as follows: Crocin significantly improved (p < 0.05) sperm motility in all breeds, except for Mediterranean Red, compared to the control group. Furthermore, 1 mM crocin reduced percentage of spermatozoa with DNA fragmentation with a marked decrement (p < 0.05) in Garganica and Saanen, as compared to the control group. Finally, intracellular ROS decreased (p < 0.01) in the crocin-treated sperm of all breeds, as compared to the control. In conclusion, supplementation of 1 mM crocin in the extender decreased oxidative stress, improving sperm motility and the DNA integrity of frozen-thawed sperm in different breeds.

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“…Higher DHA concentration promoted adverse effects on sperm motility (Losano et al 2018). Lv et al (2019) demonstrated that the higher amounts of resveratrol supplementation used did not inhibit the production of ROS and concurrently they did not diminish the cryodamage to goat sperm. Also, our finding was in accordance with Kumar et al (2015), who reported that 1% to 2% sericin had harmful effects on the sperm cells.…”

Section: Effect Of Sericin Concentration On Post-thaw Semen Qualitymentioning

confidence: 96%

Effect of semen extender supplementation with sericin on post-thaw dairy bull sperm quality and lipid peroxidation

Yangnam¹,

Chapanya²,

Vongpralub³

et al. 2021

CZECH J ANIM SCI

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Silk sericin plays a protective role in a variety of mammalian cells during cryopreservation. This study aimed to determine the effects of sericin supplementation to a freezing extender on sperm quality and lipid peroxidation in dairy bull sperm cryopreservation. Each semen sample of five Holstein-Friesian crossbred bulls was divided into four aliquots and diluted in a tris-egg yolk extender supplemented with different concentrations of sericin [0%, 0.25%, 0.5%, and 1.0% (wt/vol)]. Sperm motility (CASA), viability, acrosome integrity, mitochondrial membrane potential (fluorescent staining) and lipid peroxidation (malondialdehyde – MDA test) were analysed. The results show that the 0.25% and 0.5% sericin groups had the highest total sperm motility (P < 0.05). Sperm viability, acrosome integrity, and mitochondrial function were higher in the group supplemented with 0.25% sericin compared to the control and 1.0% (P < 0.05). Sericin supplementation with 0.25% and 0.5% significantly decreased MDA concentrations compared with the control (P < 0.05). In conclusion, supplementation of the semen freezing extender with sericin at the concentration of 0.25% significantly improved the post-thaw semen quality and reduced lipid peroxidation in Holstein-Friesian crossbred bulls.

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Improving the quality of cryopreserved goat semen with a commercial bull extender supplemented with resveratrol

Cited by 37 publications

References 67 publications

Polyphenols in Farm Animals: Source of Reproductive Gain or Waste?

Polyphenols in Farm Animals: Source of Reproductive Gain or Waste?

Crocin Improves the Quality of Cryopreserved Goat Semen in Different Breeds

Effect of semen extender supplementation with sericin on post-thaw dairy bull sperm quality and lipid peroxidation

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