Improving the Preservation of Isolated Rat Skeletal Muscles Stored for 16 Hours at 4??c1

Heijden, E.P.A. Brigitte van der; Kroese, A.B.A.; Werker, Paul M. N.; Smet, Martin De; Kon, Moshe; Bär, Dop

doi:10.1097/00007890-200004150-00017

Cited by 9 publications

(13 citation statements)

References 37 publications

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“…The primary reason for developing the hamster RET free flap model was that both contractile muscle [17] and microcirculatory measurements [15] can be used to evaluate the efficacy of preservation techniques in future studies. For example, the potential for recovery of muscle function after cold ischemia depends on recovery of both the parenchymal (muscle) cells and their vascular supply [16,29] .…”

Section: Discussionmentioning

confidence: 99%

“…We hypothesize that interventions that lead to re-establishment of local vasodilator signals will contribute to prevention of hypoperfusion and the no-reflow phenomenon. Interventions that could be employed in future research include cold preservation and several pharmacological approaches [17] .…”

Section: Discussionmentioning

confidence: 99%

“…Recently, we reported that both contractile function and endothelium-dependent blood flow regulation of the RET were impaired by I-R [14] . These findings suggested that the RET may prove useful as a free flap model to evaluate the efficacy of preservation strategies aimed to protect skeletal muscle and its vascular supply during cold preservation and reperfusion [15][16][17] . With the goal of providing further insight into how transplantation affects microvascular integrity, the aim of the present study was to understand the anatomical origin of the vascular supply to the RET and determine whether it can be isolated on a thoracodorsal vascular pedicle, preserving the feed arteries and collecting veins.…”

mentioning

confidence: 98%

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Vascular Anatomy of the Hamster Retractor Muscle with Regard to Its Microvascular Transfer

With

Vries

Kroese³

et al. 2008

Eur Surg Res

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Background: The hamster retractor muscle (RET) is used as an in vivo model in studies of skeletal muscle ischemia-reperfusion injury. The RET is unique in that the muscle can be isolated while preserving the primary vascular supply so that its contractile function can be measured simultaneously with local microvascular responses to experimental interventions. The goal of this study was to understand the anatomical origin of the vascular supply to the RET and determine whether the RET can be used as a free flap after surgical isolation of the thoracodorsal vessels. Methods: Microdissection was performed to determine the anatomy of the vasculature that supplies and drains the RET. Results: Distinct numbers and patterns of feed arteries (2–4) and collecting veins (1–3) were identified (n = 26 animals). Dye injection (n = 8) of the thoracodorsal artery demonstrated that the RET remains perfused following its isolation on the thoracodorsal pedicle. Heterotopic allograft transplantation of the RET (n = 2) was performed by anastomosing the thoracodorsal vessels to the femoral vessels using the end-to-side technique. Conclusions: The anatomical relationships indicate that the RET can be used as a free flap model for evaluating the effect of preservation strategies and transplantation on skeletal muscle microcirculation and contractile function.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 98%

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Vascular Anatomy of the Hamster Retractor Muscle with Regard to Its Microvascular Transfer

With

Vries

Kroese³

et al. 2008

Eur Surg Res

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“…The formation of highly reactive species appears to be triggered by a cold-induced increase in the cellular chelatable (or 'free'), 'redox-active' iron pool [Rauen et al, 1999Huang and Salahudeen, 2002;Rauen and de Groot, 2004], and mitochondria have been shown to be the major target of the reactive species (see 'ATP' below) Rauen et al, 2003;Salahudeen et al, 2003]. A similar mechanism also appears to be at play during cold storage of muscle tissue [Magni et al, 1994;van der Heijden et al, 2000]. To stop this iron-dependent injury, the strong, hexadentate but poorly membranepermeable iron chelator deferoxamine and the smaller, aromatic and thus more lipophilic membrane-permeable iron chelator LK 614 are present in TiProtec and its variants used in this study.…”

Section: Main Injurious Mechanisms and Counterbalancing Ingredients Omentioning

confidence: 99%

“…Especially human muscle tissue is regularly dissected in operation theaters, which are located far away from the respective diagnostic or research laboratory. Accordingly, there is an urgent need to optimize transport conditions as an impaired membrane potential [Oredsson et al, 1993] and decreased response to electrophysiological stimuli [van der Heijden et al, 2000] with subsequent loss of function and structural integrity of specimens were detected after shortterm cold storage [van der Heijden et al, 2000;Wagh et al, 2000]. So far, only few studies have been published in which muscle biopsies showed promising results regarding (ultra)structural alterations after 1-5 days when stored in a new tissue medium [Sandberg et al, 2007].…”

mentioning

confidence: 99%

Evaluation of Functional and Structural Alterations in Muscle Tissue after Short-Term Cold Storage in a New Tissue Preservation Solution

Wille

Gonder

Thiermann

et al. 2011

Cells Tissues Organs

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Storage of muscle preparations in vitro is required for the diagnosis of neuromuscular disorders and for electrophysiological tests. The current standard protocols for muscle storage or transport, i.e. placement on 0.9% NaCl-moistened gauze, lead to impaired function and structural alterations. For other tissues, however, improved preservation methods and solutions have recently been described. In this study, functional and structural alterations in the murine diaphragm were compared after storage on 0.9% NaCl-moistened gauze and after storage in different modifications of the new vascular preservation solution TiProtec®. Muscle force generation after nerve stimulation, histological parameters and ATP levels were investigated after 2.5 h of cold storage at 4°C in the different media and 0.5 h of rewarming at 25°C in Tyrode buffer. Murine diaphragms were injured during cold storage and rewarming, with the degree of the alteration being dependent on the type of solution used. There were no histological alterations and no caspase 3 activation in all groups. In contrast, diaphragms stored in the modified TiProtec solution showed markedly better performance concerning force generation after nerve stimulation (7.1 ± 1.1 cN · s) as well as higher ATP content (2.4 ± 0.7 µmol/g) and were superior to storage on 0.9% NaCl-moistened gauze (1.4 ± 0.4 cN · s; 0.3 ± 0.1 µmol/g). In conclusion, the modified TiProtec preservation solution showed promising results for short-term cold storage of murine diaphragms. For further evaluation, the transferability of these positive findings to storage conditions for muscles of other species, especially human muscle tissue, needs to be investigated.

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Effect of HTK on the microcirculation in the rat cremaster muscle during warm ischemia and reperfusion

et al. 2005

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Histidine-tryptophan-ketoglutarate (HTK) preserves rat muscle function during cold storage. We examined the effect of HTK perfusion on preservation of microvascular function during 4 h of warm ischemia and subsequent reperfusion (I/R) in the rat cremaster muscle. Leukocyte-endothelium interactions, capillary perfusion, and arteriole diameters were quantified prior to HTK-perfusion and/or ischemia, and at 0, 1, and 2 h after restoration of blood flow. In all groups, the number of rolling leukocytes increased with time, whereas I/R induced a slight increase in leukocyte adhesion. After ischemia, capillary perfusion rapidly recovered to about 50% and returned to near normal (90%) after 2 h. HTK at 22 degrees C did not affect the assessed microcirculation variables, whereas HTK at 4 degrees C reduced leukocyte rolling, but not adhesion. Therefore, microvascular function of HTK-perfused muscles was not better preserved during warm I/R than that of nonperfused muscles. Contrary to other preservation solutions, HTK perfusion in itself was not detrimental to the microcirculation.

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Improving the Preservation of Isolated Rat Skeletal Muscles Stored for 16 Hours at 4??c1

Abstract: The results showed that the addition of BDM and antioxidants (trolox and deferione) to the bathing solutions improved the preservation of the function, metabolism, and cytoarchitecture of isolated skeletal muscles after cold storage for 16 hr.

Cited by 9 publications

References 37 publications

Vascular Anatomy of the Hamster Retractor Muscle with Regard to Its Microvascular Transfer

Vascular Anatomy of the Hamster Retractor Muscle with Regard to Its Microvascular Transfer

Evaluation of Functional and Structural Alterations in Muscle Tissue after Short-Term Cold Storage in a New Tissue Preservation Solution

Effect of HTK on the microcirculation in the rat cremaster muscle during warm ischemia and reperfusion

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