“…In this study, we validated a computational workflow for image acquisition, processing, and analysis to predict the biomass yield based on vegetative indices and plant height measurements of 48,000 ryegrass plants. Previous studies indicated the use of NDVI for ranking cultivars of ryegrass (Wang et al, 2019), field pea, canola, and spring wheat grain yield (Brian McConkey et al, 2004) and lint yield in cotton (Hugie et al, 2018). Considering the plant height and NDVI as a surrogate to predict DMY of individual and plot-level plants, there is a great potential to apply our workflow to be used for ranking of genotypes and cultivars across growing seasons and years.…”