Improving the photostability of fluorescent dyes by polymer nano‐insulating layer

Abstract

Fluorescein is a traditional organic fluorescent dye that has the disadvantage of agglomeration-induced fluorescence quenching and photobleaching due to its large hydrophobic conjugate π-π system, which makes them have limitations in many fields of application. A method of polymer modification is revealed to address this issue. In this study, fluorescein molecules were modified by polycaprolactone (PCL) to obtain PCL grafting fluorescein (P x F), and subsequently assembled with PEG-PCL to obtain fluorescent nanoparticles (PPxF).PPxF would realize long term cellular fluorescence imaging. PCL polymerization induces self-assembly, which effectively avoids fluorescence quenching caused by fluorescent molecules aggregation, and at the same time well inhibit the energy transfer with oxygen to improve photostability. This method is a good way to improve the fluorescence properties of fluorescent dyes, to be suitable for all traditional organic fluorescent dyes to greatly improve their fluorescence and hydrophilic properties and enhance their application fields.

Photo Microreactor with Luminescent Fluid Mediating Solar Photochemical Synthesis

“…This characteristic would facilitate not only changing the type of luminophores to meet the different wavelength demands of different photoreactions of interest but also recovering the luminescence intensity when the luminophores (especially when small molecule organic fluorescent dyes were used) in the reactor suffer from degradation and photobleaching. 15 The former advantage is exhibited in Section 3.5, and the latter advantage is verified in this section.…”

“…In both the tube-in-tube reactor and the triple-channel reactor developed in this paper, the luminophores, acting as a promoter of the photoreaction, were dissolved in the fluid rather than being immobilized in the solid lightguide. This characteristic would facilitate not only changing the type of luminophores to meet the different wavelength demands of different photoreactions of interest but also recovering the luminescence intensity when the luminophores (especially when small molecule organic fluorescent dyes were used) in the reactor suffer from degradation and photobleaching . The former advantage is exhibited in Section , and the latter advantage is verified in this section.…”