Improving the performance of a direct photosynthetic/metabolic bio-fuel cell (DPBFC) using gene manipulated bacteria

Morishima, Keisuke; Yoshida, Makoto; Furuya, Akihiro; Moriuchi, Takeyuki; Ota, Masahiro; Furukawa, Yuji

doi:10.1088/0960-1317/17/9/s10

Cited by 16 publications

(16 citation statements)

References 13 publications

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“…In the wake of hydrogen emission suppression, R. palustris was able to internally store more reducing equivalents. Thus, the power density of the mutant was increased to 18.3 mW cm À2 while that of the wild type was 11.7 mW cm À2 [39]. Metabolites are traditionally divided into primary and secondary metabolites.…”

Section: Engineering Of Metabolic Processesmentioning

confidence: 99%

Genetically Engineered Microbial Fuel Cells

Alfonta

2010

Electroanalysis

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This article reviews the advances that were made towards the understanding and the improvement of electron transfer and communication between living cells and electrodes with a specific emphasis on microbial fuel cells and bioelectrical systems. It summarizes the efforts that were made thus far to improve electron transfer between microorganisms and electrodes using the genetically based understanding of electron transfer in such organisms and the manipulations that can be performed to improve the transfer and subsequently control over power output. Future directions in the field are also reviewed and suggested in this article.

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Section: Engineering Of Metabolic Processesmentioning

confidence: 99%

Genetically Engineered Microbial Fuel Cells

Alfonta

2010

Electroanalysis

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“…As it will be discussed later in this review, cyanobacteria are well known for hydrogen production in both dark and light and thus it could be hypothesized that the observed power output did not solely originate from the regeneration of HNQ, but also from the electrooxidation of molecular hydrogen at the platinum wire. Recently, work has been published on micromachined microbial photobioelectrochemical cells [23,24]. Methylene blue was used as redox mediator to shuttle electrons from Anabena sp.…”

Section: Microbial Photobioelectrochemical Cells Based On Exogenous Rmentioning

confidence: 99%

“…to the anode. In another recent study, the conducting polymer polyaniline was studied as an immobilized redox-mediator at the anode surface to facilitate the electron transfer from photosynthetic bacteria or cyanobacteria to the electrode [24,25]. Unfortunately, the power densities of these micromachined cells are extremely low, e.g.…”

Section: Microbial Photobioelectrochemical Cells Based On Exogenous Rmentioning

confidence: 99%

“…Unfortunately, the power densities of these micromachined cells are extremely low, e.g. 0.02 to 0.04 nW cm À2 in comparison to 10 or 18.3 mW cm À2 for gene manipulated photosynthetic bacteria used in reference [24], and an up-scale of these specially engineered m-scale systems is not intended. Therefore, at present, mphotosynthetic MFCs mainly represent academic research tools -a future application remains rather uncertain.…”

Section: Microbial Photobioelectrochemical Cells Based On Exogenous Rmentioning

confidence: 99%

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Photomicrobial Solar and Fuel Cells

Rosenbaum

Schröder

2010

Electroanalysis

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Microbial solar cells and photomicrobial fuel cells exploit the energy of light and the activity of phototrophic microorganisms to produce electricity. Whereas microbial solar cells use light as the sole energy source, photomicrobial fuel cells degrade organic matter in the presence of light. This review gives an overview about the recent developments in the field of microbial based photobiological fuel cells and microbial solar cells. It provides an insight into possible electron transfer mechanisms and elementary photobiological reaction pathways. A short outline of upcoming engineering issues is provided.

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“…[1][2][3][4][5][6][7][8] The biophotonic components derived from nature possess the ability to photosynthesize over a wide dynamic range of photonic spectra and light intensities ranging from bright sunlight conditions to dim light intensities. Current photodetection systems, such as silicon-based solar cells and charge coupled devices (CCDs) that have high relevance in biomedical and biotechnological applications, lose their efficiency under low light intensity conditions, especially in the blue range of the visible spectrum.…”

Section: Introductionmentioning

confidence: 99%

Immobilization of Functional Light Antenna Structures Derived from the Filamentous Green Bacterium Chloroflexus aurantiacus

et al. 2008

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The integration of highly efficient, natural photosynthetic light antenna structures into engineered systems while their biophotonic capabilities are maintained has been an elusive goal in the design of biohybrid photonic devices. In this study, we report a novel technique to covalently immobilize nanoscaled bacterial light antenna structures known as chlorosomes from Chloroflexus aurantiacus on both conductive and nonconductive glass while their energy transducing functionality was maintained. Chlorosomes without their reaction centers (RCs) were covalently immobilized on 3-aminoproyltriethoxysilane (APTES) treated surfaces using a glutaraldehyde linker. AFM techniques verified that the chlorosomes maintained their native ellipsoidal ultrastructure upon immobilization. Results from absorbance and fluorescence spectral analysis (where the Stokes shift to 808/810 nm was observed upon 470 nm blue light excitation) in conjunction with confocal microscopy confirm that the functional integrity of immobilized chlorosomes was also preserved. In addition, experiments with electrochemical impedance spectroscopy (EIS) suggested that the presence of chlorosomes in the electrical double layer of the electrode enhanced the electron transfer capacity of the electrochemical cell. Further, chronoamperometric studies suggested that the reduced form of the Bchl- c pigments found within the chlorosome modulate the conduction properties of the electrochemical cell, where the oxidized form of Bchl- c pigments impeded any current transduction at a bias of 0.4 V within the electrochemical cell. The results therefore demonstrate that the intact chlorosomes can be successfully immobilized while their biophotonic transduction capabilities are preserved through the immobilization process. These findings indicate that it is feasible to design biophotonic devices incorporating fully functional light antenna structures, which may offer significant performance enhancements to current silicon-based photonic devices for diverse technological applications ranging from CCD devices used in retinal implants to terrestrial and space fuel cell applications.

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Improving the performance of a direct photosynthetic/metabolic bio-fuel cell (DPBFC) using gene manipulated bacteria

Cited by 16 publications

References 13 publications

Genetically Engineered Microbial Fuel Cells

Genetically Engineered Microbial Fuel Cells

Photomicrobial Solar and Fuel Cells

Immobilization of Functional Light Antenna Structures Derived from the Filamentous Green Bacterium Chloroflexus aurantiacus

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