Improving the osteogenic potential of BMP-2 with hyaluronic acid hydrogel modified with integrin-specific fibronectin fragment

Kisiel, Marta; Martino, Mikaël M.; Ventura, Manuela; Hubbell, Jeffrey A.; Hilborn, Jöns; Ossipov, Dmitri

doi:10.1016/j.biomaterials.2012.10.015

Cited by 99 publications

(81 citation statements)

References 50 publications

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“…[44][45][46] In an ectopic bone defect model in the rat, hyaluronic acid hydrogel functionalized with the fibronectin fragment was designed for the delivery of BMP-2, and it promoted the formation of twice as much ectopic bone with a better production of collagen fibers compared to BMP-2 delivered via non-functionalized hyaluronic acid hydrogel. 47 In a critical-sized calvarial defect model in the rat, dual-delivery of BMP-2 and PDGF-BB with the adhesive fibronectin fragment was able to accelerate bone formation at low doses, whereas the growth factors delivered without the adhesive proteins produced no significant effect. 48 The two aspects of retained engineered GFs, along with natural biological adhesive proteins, may produce synergistic effects in nervous system reconstruction and repair.…”

Section: Ecm-inspired Gf Delivery Systemsmentioning

confidence: 99%

Novel biomaterial strategies for controlled growth factor delivery for biomedical applications

et al. 2017

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Growth factors (GFs) are soluble proteins secreted by cells that have the ability to regulate a variety of cellular processes and tissue regeneration. However, their translation into clinical applications is limited due to their short effective half-life, low stability, and rapid inactivation by enzymes under physiological conditions. To maximize the effectiveness of GFs and their biologically relevant applicability, a wide variety of sophisticated bio-inspired systems have been developed that augment tissue repair and cellular regeneration by controlling how much, when, and where GFs are released. Recently, protein immobilization techniques combined with nanomaterial carriers have shown promise in mimicking the natural healing cascade during tissue regeneration by augmenting the delivery and effectiveness of GFs. This review evaluates the latest techniques in direct immobilization and relevant biomaterials used for GF loading and release, including synthetic polymers, albumin, polysaccharides, lipids, mesoporous silica-based nanoparticles (NPs), and polymeric capsules. Specifically, we focus on GF-encapsulated NPs in functionalized microporous scaffolds as a promising alternative with the ability to mimic extracellular matrix (ECM) hierarchical architectures and components with high cell affinity and bioactivity. Finally, we discuss how these next-generation, advanced delivery systems have been used to enhance tissue repair and regeneration and consider future implications for their use in the field of regenerative medicine.

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Section: Ecm-inspired Gf Delivery Systemsmentioning

confidence: 99%

Novel biomaterial strategies for controlled growth factor delivery for biomedical applications

et al. 2017

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Section: Gelatinmentioning

confidence: 99%

“…tried to mimic the ECM copolymerizing HA and gelatin. They added a thiol group to the Hyaluronan, in this way they were able to crosslinked modified-Hyaluronan with modified-Gelatin by disulfide bonds [130].Pectin: Another polysaccharides used in tissue engineering hydrogels is pectin. It is obtained from cells walls after a low pH, high temperature processing.…”

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confidence: 99%

History and Applications of Hydrogels

Yahia¹

2015

J Biomed Sci

160

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Hydrogels have existed for more than half century, providing one of the earliest records of crosslinked hydroxyethyl methacrylate (HEMA) hydrogels. Today, hydrogels still fascinate material scientists and biomedical researchers and great strides have been made in terms of their formulations and applications. As a class of material, hydrogels are unique, they consist of a self-supporting, waterswollen three-dimensional (3D) viscoelastic network which permits the diffusion and attachment of molecules and cells. However, hydrogels have recently drawn great attention for use in a wide variety of biomedical applications such as cell therapeutics, wound healing, cartilage/bone regeneration and the sustained release of drugs. This is due to their biocompatibility and the similarity of their physical properties to natural tissue. This review aims to give an overview of the historic and the recent design concept of hydrogels and their several applications based on the old and the most recent publications in this field. Journal of Biomedical Sciences ISSN 2254-609XThis Article is Available in: www.jbiomeds.com 2 Hydrogels can be classified into two distinct categories, the natural and the synthetic hydrogels. Natural hydrogels include collagen, fibrin, hyaluronic acid, matrigel, and derivatives of natural materials such as chitosan, alginate and skill fibers. They remain the most physiological hydrogels as they are components of the extracellular matrix (ECM) in vivo. Two main drawbacks of natural hydrogels, however, make their final microstructures and properties difficult to control reproducibly between experiments. First, the fine details of their mechanical properties and their dependence on polymerization or gelation conditions are often poorly understood. Second, due to their natural origin (bovine fibrinogen, rat tail collagen… their composition may vary from one batch to another.In contrast, synthetic hydrogels such as poly (ethylene glycol) diacrylate, poly(acryl amide), poly(vinyl alcohol) are more reproducible, although their final structure can also depend on polymerization conditions in a subtle way, so that a rigorous control of the preparation protocol, including temperature and environment control, may be necessary. Generally speaking, synthetic hydrogels offer more flexibility for tuning chemical composition and mechanical properties; users can, for example vary the concentration or molecular weight of the precursor, or alter the percentage of crosslinkers. They can also be selected or tuned to be hydrolysable or biodegradable over variable periods of time.Hydrogels may be chemically stable or they may degrade and eventually disintegrate and dissolve. They are called «reversible» or «physical» gels when the networks are held together by molecular entanglements, and/or secondary forces including ionic, H-bonding or hydrophobic forces (Figure 2) [3]. Physical hydrogels are not homogeneous, since clusters of molecular entanglements, or hydrophobically-or ionically-associated domains, can create in h...

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“…The constructs were impacted and implanted subcutaneously and after 4 weeks perfused in vivo with Microfil prior to µCT scanning (Bolland et al, 2008). The subcutaneous implants provide a rapid screening model for "biocompatibility" and osteo-inductivity/-conductivity and have been used for bioactive materials carrying growth factors such as Bone morphogenetic proteins (BMPs) (Kisiel et al, 2013). Several implants can be compared in the same individual giving paired data and hence the possibility to reduce the number of animals used while achieving valid information.…”

Section: Subcutaneous Modelsmentioning

confidence: 99%

A surprisingly poor correlation between in vitro and in vivo testing of biomaterials for bone regeneration: results of a multicentre analysis

Hulsart‐Billström

Dawson²,

Hofmann

et al. 2016

eCM

117

101

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New regenerative materials and approaches need to be assessed through reliable and comparable methods for rapid translation to the clinic. There is a considerable need for proven in vitro assays that are able to reduce the burden on animal testing, by allowing assessment of biomaterial utility predictive of the results currently obtained through in vivo studies. The purpose of this multicentre review was to investigate the correlation between existing in vitro results with in vivo outcomes observed for a range of biomaterials. Members from the European consortium BioDesign, comprising 8 universities in a European multicentre study, provided data from 36 in vivo studies and 47 in vitro assays testing 93 different biomaterials. The outcomes of the in vitro and in vivo experiments were scored according to commonly recognised measures of success relevant to each experiment. The correlation of in vitro with in vivo scores for each assay alone and in combination was assessed. A surprisingly poor correlation between in vitro and in vivo assessments of biomaterials was revealed indicating a clear need for further development of relevant in vitro assays. There was no significant overall correlation between in vitro and in vivo outcome. The mean in vitro scores revealed a trend of covariance to in vivo score with 58 %. The inadequacies of the current in vitro assessments highlighted here further stress the need for the development of novel approaches to in vitro biomaterial testing and validated pre-clinical pipelines.

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Improving the osteogenic potential of BMP-2 with hyaluronic acid hydrogel modified with integrin-specific fibronectin fragment

Cited by 99 publications

References 50 publications

Novel biomaterial strategies for controlled growth factor delivery for biomedical applications

Novel biomaterial strategies for controlled growth factor delivery for biomedical applications

History and Applications of Hydrogels

A surprisingly poor correlation between in vitro and in vivo testing of biomaterials for bone regeneration: results of a multicentre analysis

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