Improving the osteogenesis of rat mesenchymal stem cells by chitosan-based-microRNA nanoparticles

Wu, Guangsheng; Feng, Chao; Guangyan, Hui; Wang, Zhongshan; Tan, Jun; Luo, Lankun; Xue, Peng; Wang, Qintao; Chen, Xiguang

doi:10.1016/j.carbpol.2015.11.044

Cited by 61 publications

(42 citation statements)

References 35 publications

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“…[15][16][17] The positive charges of CS contribute to electrostatic interactions with negatively charged miRNAs for the delivery of transfection agents, which are also responsible for their capacity to interact with negatively charged cell surfaces. 18 HA is an anionic polysaccharide naturally found in humans, which has been widely used for bioabsorbable scaffolds and drug delivery carriers.…”

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confidence: 99%

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confidence: 99%

“…16 An optimal mixture with a CS:TPP:HA volume ratio of 1:0.15:0.1 was adopted to prepare NPs. 16 NPs were formed instantaneously upon the dropwise addition of TPP/HA solution to a fixed volume of CS solution under magnetic stirring for 10 minutes. CS/HA/ miR-21 NPs were prepared by adding the required volume of 50 μM miR-21s to the CS/HA NP systems by gentle pipetting to form complexes of a selected N/P ratio, which was defined as the molar ratio of the positive CS amino group to the negative miRNA phosphate group.…”

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confidence: 99%

“…22 However, the inclusion of an anionic polymer (HA) could increase the stability of the particles and increase the gene transfection efficacy, according to previous reports. 16,20 In addition, HA could bind to CD44 on the hBMMSC cellular receptors, which would induce endocytosis in a targeted manner. 21 Liu et al 28 found that HA could improve cellular uptake through its binding receptor, leading to easy release of the transfection agents after internalization.…”

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confidence: 99%

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Improving the osteogenesis of human bone marrow mesenchymal stem cell sheets by microRNA-21-loaded chitosan/hyaluronic acid nanoparticles via reverse transfection

Wang

Wu²,

Wei

et al. 2016

IJN

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Cell sheet engineering has emerged as a novel approach to effectively deliver seeding cells for tissue regeneration, and developing human bone marrow mesenchymal stem cell (hBMMSC) sheets with high osteogenic ability is a constant requirement from clinics for faster and higher-quality bone formation. In this work, we fabricated biocompatible and safe chitosan (CS)/hyaluronic acid (HA) nanoparticles (NPs) to deliver microRNA-21 (miR-21), which has been proved to accelerate osteogenesis in hBMMSCs; then, the CS/HA/miR-21 NPs were cross-linked onto the surfaces of culture plates with 0.2% gel solution to fabricate miR-21-functionalized culture plates for reverse transfection. hBMMSC sheets were induced continuously for 14 days using a vitamin C-rich method on the miR-21-functionalized culture plates. For the characterization of CS/HA/miR-21 NPs, the particle size, zeta potential, surface morphology, and gel retardation were sequentially investigated. Then, the biological effects of hBMMSC sheets on the miR-21-functionalized culture plates were evaluated. The assay results demonstrated that the hBMMSC sheets could be successfully induced via the novel reverse transfection approach, and miR-21 delivery significantly enhanced the in vitro osteogenic differentiation of hBMMSC sheets in terms of upregulating calcification-related gene expression and enhancing alkaline phosphatase production, collagen secretion, and mineralized nodule formation. The enhanced osteogenic activity of hBMMSC sheets might promisingly lead to more rapid and more robust bone regeneration for clinical use.

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confidence: 99%

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confidence: 99%

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confidence: 99%

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Improving the osteogenesis of human bone marrow mesenchymal stem cell sheets by microRNA-21-loaded chitosan/hyaluronic acid nanoparticles via reverse transfection

Wang

Wu²,

Wei

et al. 2016

IJN

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“…Given the properties of biocompatibility and biodegradability of the nanoparticles, many studies now exist on the use of nanomaterials to prolong the expression of proteins for improved angiogenesis and recruitment of stem cells for tissue and organ regeneration (i.e., cardiac and vascular tissues [57,58]). Among the others, carbon nanotubes (CNTs), PEGylated multi walled carbon nanotubes (MWCNTs) [59], chitosan nanoparticles (CSNPs) [60], poly(lactic-co-glycolic acid) (PLGA) scaffolds [61], polycaprolactones (PCL) scaffolds [62], poly-L-lactic acid (PLLA) [63], polyethyleneimine (PEI) have been already explored [64].…”

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confidence: 99%

Nanotechnology Advances in Drug Delivery

Velluto¹,

Ricordi²

2017

NanoWorld J

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Drug delivery emerged as a discipline to solve problems associated with the majority of the current drugs, such as poor water solubility, poor physical stability, poor absorption and side effects. Large pharmaceutical companies are investing in drug delivery technologies to find better ways to administer existing drugs rather than designing new products. The secret to a successful drug delivery system, one that allows controlled release over a prolonged period of time, is in the carrier. An ideal drug carrier must be biocompatible, biodegradable, water friendly, selective, easy to prepare, stable, cheap, and finally, ultra-small. Therefore, the interdisciplinary field of nanotechnology and nanomaterials is playing a big role in drug delivery by providing new tools to develop ideal nanocarriers and find appropriate solutions for medical problems. In this review, we briefly recapitulate the history of nanomaterials in drug delivery, explore their unique properties, and report an example of the design and development of polymerbased nanomaterials. We also revisit the most challenging applications of drug delivery for cancer treatment, cell and tissue transplantations and stem cell therapies. Overall, nanotechnology-based drug delivery systems administered by different routes can be considered promising tools to improve patient compliance and achieve better therapeutic outcomes in critical illnesses.

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Nanoengineered Endocytic Biomaterials for Stem Cell Therapy

Wang,

Sun,

Liu

et al. 2024

Adv Funct Materials

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Stem cells, ideal for the tissue repair and regeneration, possess extraordinary capabilities of multidirectional differentiation and self‐renewal. However, the limited spontaneous differentiation potential makes it challenging to harness them for tissue repair without external intervention. Although conventional approaches using biomolecules, small organic molecules, and ions have shown specific and effective functions, they face challenges such as in vivo diffusion and degradation, poor internalization, and side effects on adjacent cells. Nanoengineered biomaterials offer a solution by solidifying and nanosizing these soluble regulating molecules and ions, facilitating their uptake by stem cells. Once inside lysosomes, these nanoparticles release their contents in a controlled “molecule or ion storm,” efficiently altering the intracellular biological and chemical microenvironment to tune the differentiation of stem cells. This newly emerged approach for regulating stem cell fate has attracted much attention in recent years. This method has shown promising results and is poised to enhance clinical stem cell therapy. This review provides an overview of the design principles for nanoengineered biomaterials, discusses the categories and characteristics of nanoparticles, summarizes the application of nanoparticles in tissue repair and regeneration, and discusses the direction of nanoparticle‐enhanced stem cell therapy and prospects for its clinical application in regenerative medicine.

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Improving the osteogenesis of rat mesenchymal stem cells by chitosan-based-microRNA nanoparticles

Cited by 61 publications

References 35 publications

Improving the osteogenesis of human bone marrow mesenchymal stem cell sheets by microRNA-21-loaded chitosan/hyaluronic acid nanoparticles via reverse transfection

Improving the osteogenesis of human bone marrow mesenchymal stem cell sheets by microRNA-21-loaded chitosan/hyaluronic acid nanoparticles via reverse transfection

Nanotechnology Advances in Drug Delivery

Nanoengineered Endocytic Biomaterials for Stem Cell Therapy

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