Improving the Nutritional Value of Potatoes by Conventional Breeding and Genetic Modification

Bradshaw, J. E.

doi:10.1007/978-3-030-04609-5_3

Cited by 12 publications

(4 citation statements)

References 154 publications

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“…This includes techniques like marker-assisted selection, where specific genetic markers associated with desired traits, such as high iron content, are identified and used to guide the breeding process. Genetic engineering may also be employed to introduce or enhance specific genes responsible for nutrient accumulation [88][89][90].…”

Section: Molecular Approach 431 Transgenic Cultivarsmentioning

confidence: 99%

Potato Biofortification: A Systematic Literature Review on Biotechnological Innovations of Potato for Enhanced Nutrition

Agrawal,

Kumar,

Gupta

et al. 2024

Horticulturae

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Potato biofortification is a comprehensive approach aimed at enhancing the nutritional content of potatoes, addressing widespread nutrient deficiencies and contributing to global food security. This systematic review examines the existing literature on various aspects of potato biofortification, encompassing genetic, agronomic, and biotechnological strategies. The review highlights the nutritional significance of potatoes, emphasizing their role as a staple food in many regions. Genetic approaches to biofortification involve the identification and use of natural variations in potato germplasm to develop varieties with elevated levels of essential nutrients. This includes targeting key micronutrients, such as iron, zinc, and vitamins, through traditional breeding methods. The review explores the genetic diversity within potato germplasm and the potential for breeding programs to develop nutrient-rich varieties. Agronomic practices play a crucial role in potato biofortification, with studies demonstrating the impact of tuber priming and the application of mineral fertilizers on nutrient concentrations in potatoes. The review delves into the intricacies of agronomic biofortification, emphasizing the importance of precise dosages and timing for optimal results. Biotechnological tools, including transgenic and non-transgenic approaches, are discussed in the context of potato biofortification. The review evaluates the efficiency and ethical considerations associated with the development of biofortified transgenic potatoes and emphasizes the significance of non-transgenic approaches in addressing consumer concerns and regulatory barriers. Overall, this systematic review provides a comprehensive overview of the current state of potato biofortification research. It synthesizes findings from diverse studies, offering insights into the potential of biofortified potatoes to address hidden hunger and contribute to improved nutritional outcomes. This review also identifies knowledge gaps and areas for future research, guiding the direction of efforts to harness the full potential of potato biofortification for global food and nutrition security.

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Section: Molecular Approach 431 Transgenic Cultivarsmentioning

confidence: 99%

Potato Biofortification: A Systematic Literature Review on Biotechnological Innovations of Potato for Enhanced Nutrition

Agrawal,

Kumar,

Gupta

et al. 2024

Horticulturae

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“…Potato seed production currently faces considerable challenges, such as low multiplication rates, high storage and transportation costs, virus carry-over, and performance reductions over generations [ 8 ]. Heterozygous genotypes cannot be sexually reproduced to form botanical seeds or TPS in light of the fact that parental chromosomes will recombine to produce different combinations and the same genotype cannot be reconstituted [ 9 ]. The favorable allele combinations are lost in the sexual cycle due to the segregation of traits.…”

Section: Introductionmentioning

confidence: 99%

Generation of Asynaptic Mutants in Potato by Disrupting StDMC1 Gene Using RNA Interference Approach

Kumar

Siddappa

Bhardwaj

et al. 2023

Life

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Fixing the genomic composition and multiplication through true potato seed (TPS) is an important challenge in autotetraploid potato. Disrupted meiotic cDNA (DMC1) is a meiotic gene that plays a central role in DNA recombination through crossing over in meiosis. Using the Arabidopsis DMC1 (AtDMC1) gene sequence, we retrieved Solanum tuberosum DMC1(StDMC1) from the diploid potato genome, and subsequently, sense and antisense regions of the StDMC1 gene were amplified in potato cv. Kufri Jyoti. The sense and antisense fragments were confirmed by Sanger-sequencing and cloned in the pRI101 vector. Agrobacterium-mediated transformation of the RNAi construct resulted in 44% transformation efficiency, and a total of 137 mutant lines were obtained. These mutant lines were further validated through pollen viability testing, and selected lines were used for gene expression analysis. The acetocarmine-based pollen staining showed reduced pollen viability ranging from 14 to 21% in four DMC1 mutant lines (DMC4-37, DMC4-41, DMC6-20, and DMC6-21), as compared to the Kufri Jyoti control plants, which on average exhibited 78% pollen viability. The phenotypic data was supported by the reduced expression of the StDMC1 gene in these four mutant lines compared to the control Kufri Jyoti. The results confirmed the generation of StDMC1 knockdown lines. This is the first report of StDMC1 mutant line generation in tetraploid potatoes and will be a step forward in generating non-recombinant mutants through sexual reproduction in potatoes.

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“…These cultivars often have lower yields and different traits than cultivars typically grown by the industry (e.g., size, shape, color, etc. ), but they excel at value-added traits [ 8 – 10 ]. In potatoes, one novel value-added trait is related to human health.…”

Section: Introductionmentioning

confidence: 99%

Grower decision-making factors in adoption of specialty cultivars: A case study of potatoes in the San Luis Valley

et al. 2022

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Potatoes are the most consumed vegetable worldwide and play an important role in the U.S. economy. Growers make critical decisions each year in choosing which cultivar to grow, based on factors such as yield, resilience to the growing environment, and utility in the food industry. Current research supports the finding that less-common specialty cultivars (SCs) have benefits for human health. However, growers have been slow to adopt SCs into mainstream operations. Here, we identify major factors in the decision-making process that determine whether a population of growers in the San Luis Valley, Colorado, a major potato-growing region, adopt SC potatoes. We used a combination of ethnographic techniques and quantitative methods to examine drivers of adoption. The data demonstrate grower perceptions within potato farming and the complexity of interacting factors in decision-making. An integration of the Theory of Planned Behavior, Rational Expectation Hypothesis, and Diffusion of Innovation models identifies economic and social factors that influence grower decision-making. Growers that were more aware of specialty cultivar innovation and associated consumer demand were more open to SCs adoption. Other influencing factors include a grower’s experience selling a SC in the previous year and access to diverse markets. Based on these data, we developed a new model to explain grower decision-making processes in adopting SCs. The model demonstrates that one current barrier to adoption is access to buyers, including warehouses, retailers, and households. Taken together, this research demonstrates how rational expectations stem from economic outcomes, knowledge, and experience in the potato industry. These results are important in helping to consider opportunities for growers to access new, higher value markets, while also improving consumer access to nutritious cultivars.

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Improving the Nutritional Value of Potatoes by Conventional Breeding and Genetic Modification

Cited by 12 publications

References 154 publications

Potato Biofortification: A Systematic Literature Review on Biotechnological Innovations of Potato for Enhanced Nutrition

Potato Biofortification: A Systematic Literature Review on Biotechnological Innovations of Potato for Enhanced Nutrition

Generation of Asynaptic Mutants in Potato by Disrupting StDMC1 Gene Using RNA Interference Approach

Grower decision-making factors in adoption of specialty cultivars: A case study of potatoes in the San Luis Valley

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