Improving the content of high value compounds in Nordic Desmodesmus microalgal strains

Mehariya, Sanjeet; Plöhn, Martin; León-Vaz, Antonio; Patel, Alok; Funk, Christiane

doi:10.1016/j.biortech.2022.127445

Cited by 12 publications

(6 citation statements)

References 49 publications

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“…Similarly, the strain Isochrysis galbana growing under 20% and 35% of salinity levels showed preferential growth and fucoxanthin production [ 119 ]. Nearly three folds augmentation of carotenoid production in Desmodesmus has been reported after exposure to 40% of NaCl with moderate illumination of light, as reported by Mehariya et al [ 120 ]. The optimum NaCl concentration of 1% accelerates carotenoid accumulation in the strain Scenedesmus [ 121 ].…”

Section: Optimization Of Culture Parameters For Enhancement Of Microa...supporting

confidence: 59%

Microalgal Carotenoids: Therapeutic Application and Latest Approaches to Enhance the Production

Sirohi

Verma²,

Singh

et al. 2022

CIMB

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Microalgae are microscopic photosynthetic organisms frequently found in fresh and marine water ecosystems. Various microalgal species have been considered a reservoir of diverse health-value products, including vitamins, proteins, lipids, and polysaccharides, and are broadly utilized as food and for the treatment of human ailments such as cancer, cardiovascular diseases, allergies, and immunodeficiency. Microalgae-derived carotenoids are the type of accessory pigment that possess light-absorbing potential and play a significant role in metabolic functions. To date, nearly a thousand carotenoids have been reported, but a very less number of microalgae have been used for the commercial production of carotenoids. This review article briefly discussed the carotenoids of microalgal origin and their therapeutic application. In addition, we have briefly compiled the optimization of culture parameters used to enhance microalgal carotenoid production. In addition, the latest biotechnological approaches used to improve the yields of carotenoid has also been discussed.

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Section: Optimization Of Culture Parameters For Enhancement Of Microa...supporting

confidence: 59%

Microalgal Carotenoids: Therapeutic Application and Latest Approaches to Enhance the Production

Sirohi

Verma²,

Singh

et al. 2022

CIMB

View full text Add to dashboard Cite

show abstract

“…Microalgae biomass was determined by microplate reader Synergid H1 (BioTek, USA) spectra-fluorometer on every alternate day, 200 μL culture loaded on 96 well plates, and the absorbance recorded at 680 nm for calculating biomass concentration from equation (8) (9) (10) and (11) [21].…”

Section: Biomass Lipid and Pigment Analysismentioning

confidence: 99%

“…Intercellular carotenoid synthesis increases during light stress to protect cells from photoinhibition [9]. Consequently, different microalgal species require different levels of light for growth and biomolecule synthesis [10]. Since the nutrients in the growth medium have a major influence on the development of microalgae, optimizing appropriate growth media is critical for industrial applications.…”

Section: Introductionmentioning

confidence: 99%

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Combinatorial Effect of Multiple Variables on Carotenoids and Lipids Up-Regulation in <em>Monoraphidium</em> sp. for Pharmacological and Nutraceutical Applications

Yadav¹,

Kumar²,

Nikalje³

et al. 2023

Preprint

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Carotenoids are essential pigments that play a vital role in the photosynthetic process of algae and are important for human health and nutrition. Despite their potential value, algal carotenoids are being neglected by biofuel industries. Recent research is aimed at producing microalgal lipids and carotenoids simultaneously under abiotic stresses. In this study, the effect of different abiotic stress factors such as Nitrogen (N), Potassium (K), Phosphorous (P), Sulphur (S), light intensity, and photoperiod on biomass, lipid, and carotenoids synthesis in Monoraphidium sp. (MP) were explored utilizing a statistical method. In addition, 2, 7-dichlorofluorescein diacetate (DCF-DA) mediated spectrofluorimetry was employed to detect the ele-vation in ROS level. The results revealed that variations in nutrients and light con-ditions are the key factors in the up-regulation of biomolecules lipids and carote-noids in the algae. The carotenoids profiling was done by Ultra Performance Liquid Chromatography Quadrupole Time-of-Flight Mass Spectroscopy (UPLC-Q-ToF-MS). Among consisting carotenoids violaxanthin, astaxanthin, and beta-carotene were enhanced by 1.3 folds, 1.19 folds, 1.08 folds, while lutein was declined by 1.32 folds under stressed condition compared to control. The identified carotenoids are rec-ognized for their importance in the pharmaceutical and nutraceutical industries, highlighting the significant role of MP in these sectors.

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“…Intercellular carotenoid synthesis increases during light stress to protect cells from photoinhibition [10]. Consequently, different microalgal species require different levels of light for growth and biomolecule synthesis [11]. Since the nutrients in the growth medium have a major influence on the development of microalgae, optimizing an appropriate growth media is critical for industrial scale-up.…”

Section: Introductionmentioning

confidence: 99%

Combinatorial Effect of Multiple Abiotic Factors on Up-Regulation of Carotenoids and Lipids in Monoraphidium sp. for Pharmacological and Nutraceutical Applications

et al. 2023

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Carotenoids have attracted significant interest due to their potential use in human health and nutrition, and their global demand increases by 3.7% annually. Currently, synthetic carotenoids dominate the market, but possess challenges such as low antioxidant ability, issues with health benefits, and safety concerns. Microalgae are potential producers of natural carotenoids with extraordinary antioxidant properties, although the yield is often low in their natural cycle. The present investigation aimed to analyze the effect of multiple abiotic factors on enhancing algal carotenoids synthesis and other metabolites without affecting growth. The potential microalgae Monoraphidium sp. were grown under altered nutrient and light conditions employing RSM-CCD. The optimized conditions, such as Nitrogen (5 g·L−1), Phosphorus +Potassium (250 mg·L−1), Sulphur (70 mg·L−1), and light (137.5 µmol·m−2·S−1), resulted in increased biomass (1357.36 mg·L−1), lipid accumulation (40.28% of dry biomass), and carotenoids (16.26 µg·mL−1) as compared to the control conditions. The total carotenoids fraction consisted of astaxanthin (14.8%), violaxanthin (3.61%), lutein, (45.12%), 9-cis-β carotene (7.62%), and β-carotene-5,6-epoxide (24.21%). Among them, violaxanthin (1.32-fold), astaxanthin (1.19-fold), 9-cis- β carotene (1.07-fold), and β-carotene-5,6-epoxide (1.08-fold) content increased while lutein (1.32-fold) content decreased significantly. The improvement in algal carotenoids under novel culture conditions provides a significant advantage to pharmaceutical and nutraceutical industries.

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Improving the content of high value compounds in Nordic Desmodesmus microalgal strains

Cited by 12 publications

References 49 publications

Microalgal Carotenoids: Therapeutic Application and Latest Approaches to Enhance the Production

Microalgal Carotenoids: Therapeutic Application and Latest Approaches to Enhance the Production

Combinatorial Effect of Multiple Variables on Carotenoids and Lipids Up-Regulation in <em>Monoraphidium</em> sp. for Pharmacological and Nutraceutical Applications

Combinatorial Effect of Multiple Abiotic Factors on Up-Regulation of Carotenoids and Lipids in Monoraphidium sp. for Pharmacological and Nutraceutical Applications

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